Rhizomucor miehei lipase immobilized on reinforced chitosan-chitin nanowhiskers support for synthesis of eugenyl benzoate
An alternative environmentally benign support was prepared from chitosan–chitin nanowhiskers (CS/CNWs) for covalent immobilization of Rhizomucor miehei lipase (RML) to increase the operational stability and recyclability of RML in synthesizing eugenyl benzoate. The CS/CNWs support and RML-CS/CNWs we...
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| Format: | Article |
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Taylor and Francis Ltd.
2018
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| author | Abdul Manan, Fatin Myra Attan, Nursyafreena Widodo, Nashi Enein, H. Aboul Wahab, R. A. |
| author_facet | Abdul Manan, Fatin Myra Attan, Nursyafreena Widodo, Nashi Enein, H. Aboul Wahab, R. A. |
| author_sort | Abdul Manan, Fatin Myra |
| collection | ePrints |
| description | An alternative environmentally benign support was prepared from chitosan–chitin nanowhiskers (CS/CNWs) for covalent immobilization of Rhizomucor miehei lipase (RML) to increase the operational stability and recyclability of RML in synthesizing eugenyl benzoate. The CS/CNWs support and RML-CS/CNWs were characterized using X-ray diffraction, fluorescent microscopy, and Fourier transform infrared spectroscopy. Efficiency of the RML-CS/CNWs was compared to the free RML to synthesize eugenyl benzoate for parameters: reaction temperature, stirring rate, reusability, and thermal stability. Under optimal experimental conditions (50°C, 250 rpm, catalyst loading 3 mg/mL), a twofold increase in yield of eugenyl benzoate was observed for RML-CS/CNWs as compared to free RML, with the former achieving maximum yield of the ester at 62.1% after 5 hr. Results demonstrated that the strategy adopted to prepare RML-CS/CNWs was useful, producing an improved and prospectively greener biocatalyst that supported a sustainable process to prepare eugenyl benzoate. Moreover, RML-CS/CNWs are biodegradable and perform esterification reactions under ambient conditions as compared to the less eco-friendly conventional acid catalyst. This research provides a facile and promising approach for improving activity of RML in which the resultant RML-CS/CNWs demonstrated good operational stability for up to eight successive esterification cycles to synthesize eugenyl benzoate. |
| first_indexed | 2024-03-05T20:38:23Z |
| format | Article |
| id | utm.eprints-86248 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T20:38:23Z |
| publishDate | 2018 |
| publisher | Taylor and Francis Ltd. |
| record_format | dspace |
| spelling | utm.eprints-862482020-08-31T13:57:18Z http://eprints.utm.my/86248/ Rhizomucor miehei lipase immobilized on reinforced chitosan-chitin nanowhiskers support for synthesis of eugenyl benzoate Abdul Manan, Fatin Myra Attan, Nursyafreena Widodo, Nashi Enein, H. Aboul Wahab, R. A. QD Chemistry An alternative environmentally benign support was prepared from chitosan–chitin nanowhiskers (CS/CNWs) for covalent immobilization of Rhizomucor miehei lipase (RML) to increase the operational stability and recyclability of RML in synthesizing eugenyl benzoate. The CS/CNWs support and RML-CS/CNWs were characterized using X-ray diffraction, fluorescent microscopy, and Fourier transform infrared spectroscopy. Efficiency of the RML-CS/CNWs was compared to the free RML to synthesize eugenyl benzoate for parameters: reaction temperature, stirring rate, reusability, and thermal stability. Under optimal experimental conditions (50°C, 250 rpm, catalyst loading 3 mg/mL), a twofold increase in yield of eugenyl benzoate was observed for RML-CS/CNWs as compared to free RML, with the former achieving maximum yield of the ester at 62.1% after 5 hr. Results demonstrated that the strategy adopted to prepare RML-CS/CNWs was useful, producing an improved and prospectively greener biocatalyst that supported a sustainable process to prepare eugenyl benzoate. Moreover, RML-CS/CNWs are biodegradable and perform esterification reactions under ambient conditions as compared to the less eco-friendly conventional acid catalyst. This research provides a facile and promising approach for improving activity of RML in which the resultant RML-CS/CNWs demonstrated good operational stability for up to eight successive esterification cycles to synthesize eugenyl benzoate. Taylor and Francis Ltd. 2018-01 Article PeerReviewed Abdul Manan, Fatin Myra and Attan, Nursyafreena and Widodo, Nashi and Enein, H. Aboul and Wahab, R. A. (2018) Rhizomucor miehei lipase immobilized on reinforced chitosan-chitin nanowhiskers support for synthesis of eugenyl benzoate. Preparative Biochemistry & Biotechnology, 48 (1). pp. 92-102. ISSN 1082-6068 http://dx.doi.org/10.1080/10826068.2017.1405021 |
| spellingShingle | QD Chemistry Abdul Manan, Fatin Myra Attan, Nursyafreena Widodo, Nashi Enein, H. Aboul Wahab, R. A. Rhizomucor miehei lipase immobilized on reinforced chitosan-chitin nanowhiskers support for synthesis of eugenyl benzoate |
| title | Rhizomucor miehei lipase immobilized on reinforced chitosan-chitin nanowhiskers support for synthesis of eugenyl benzoate |
| title_full | Rhizomucor miehei lipase immobilized on reinforced chitosan-chitin nanowhiskers support for synthesis of eugenyl benzoate |
| title_fullStr | Rhizomucor miehei lipase immobilized on reinforced chitosan-chitin nanowhiskers support for synthesis of eugenyl benzoate |
| title_full_unstemmed | Rhizomucor miehei lipase immobilized on reinforced chitosan-chitin nanowhiskers support for synthesis of eugenyl benzoate |
| title_short | Rhizomucor miehei lipase immobilized on reinforced chitosan-chitin nanowhiskers support for synthesis of eugenyl benzoate |
| title_sort | rhizomucor miehei lipase immobilized on reinforced chitosan chitin nanowhiskers support for synthesis of eugenyl benzoate |
| topic | QD Chemistry |
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