Raw oil palm frond leaves as cost-effective substrate for cellulase and xylanase productions by Trichoderma asperellum UC1 under solid-state fermentation
Production of cellulases and xylanase by a novel Trichoderma asperellum UC1 (GenBank accession no. MF774876)under solid state fermentation (SSF)of raw oil palm frond leaves (OPFL)was optimized. Under optimum fermentation parameters (30 °C, 60–80% moisture content, 2.5 × 106 spores/g inoculum size)ma...
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| Format: | Article |
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Elsevier Ltd.
2019
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| author | Ezeilo, Uchenna R. Lee, Chew Tin Huyop, Fahrul Zakaria, Iffah Izzati Abdul Wahab, Roswanira |
| author_facet | Ezeilo, Uchenna R. Lee, Chew Tin Huyop, Fahrul Zakaria, Iffah Izzati Abdul Wahab, Roswanira |
| author_sort | Ezeilo, Uchenna R. |
| collection | ePrints |
| description | Production of cellulases and xylanase by a novel Trichoderma asperellum UC1 (GenBank accession no. MF774876)under solid state fermentation (SSF)of raw oil palm frond leaves (OPFL)was optimized. Under optimum fermentation parameters (30 °C, 60–80% moisture content, 2.5 × 106 spores/g inoculum size)maximum CMCase, FPase, β-glucosidase and xylanase activity were recorded at 136.16 IU/g, 26.03 U/g, 130.09 IU/g and 255.01 U/g, respectively. Cellulases and xylanase were produced between a broad pH range of pH 6.0–12.0. The enzyme complex that comprised of four endo-β-1,4-xylanases and endoglucanases, alongside exoglucanase and β-glucosidase showed thermophilic and acidophilic characteristics at 50–60 °C and pH 3.0–4.0, respectively. Glucose (16.87 mg/g)and fructose (18.09 mg/g)were among the dominant sugar products from the in situ hydrolysis of OPFL, aside from cellobiose (105.92 mg/g)and xylose (1.08 mg/g). Thermal and pH stability tests revealed that enzymes CMCase, FPase, β-glucosidase and xylanase retained 50% residual activities for up to 15.18, 4.06, 17.47 and 15.16 h of incubation at 60 °C, as well as 64.59, 25.14, 68.59 and 19.20 h at pH 4.0, respectively. Based on the findings, it appeared that the unique polymeric structure of raw OPFL favored cellulases and xylanase productions. |
| first_indexed | 2024-03-05T20:43:10Z |
| format | Article |
| id | utm.eprints-87854 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T20:43:10Z |
| publishDate | 2019 |
| publisher | Elsevier Ltd. |
| record_format | dspace |
| spelling | utm.eprints-878542020-11-30T13:28:38Z http://eprints.utm.my/87854/ Raw oil palm frond leaves as cost-effective substrate for cellulase and xylanase productions by Trichoderma asperellum UC1 under solid-state fermentation Ezeilo, Uchenna R. Lee, Chew Tin Huyop, Fahrul Zakaria, Iffah Izzati Abdul Wahab, Roswanira QH Natural history TP Chemical technology Production of cellulases and xylanase by a novel Trichoderma asperellum UC1 (GenBank accession no. MF774876)under solid state fermentation (SSF)of raw oil palm frond leaves (OPFL)was optimized. Under optimum fermentation parameters (30 °C, 60–80% moisture content, 2.5 × 106 spores/g inoculum size)maximum CMCase, FPase, β-glucosidase and xylanase activity were recorded at 136.16 IU/g, 26.03 U/g, 130.09 IU/g and 255.01 U/g, respectively. Cellulases and xylanase were produced between a broad pH range of pH 6.0–12.0. The enzyme complex that comprised of four endo-β-1,4-xylanases and endoglucanases, alongside exoglucanase and β-glucosidase showed thermophilic and acidophilic characteristics at 50–60 °C and pH 3.0–4.0, respectively. Glucose (16.87 mg/g)and fructose (18.09 mg/g)were among the dominant sugar products from the in situ hydrolysis of OPFL, aside from cellobiose (105.92 mg/g)and xylose (1.08 mg/g). Thermal and pH stability tests revealed that enzymes CMCase, FPase, β-glucosidase and xylanase retained 50% residual activities for up to 15.18, 4.06, 17.47 and 15.16 h of incubation at 60 °C, as well as 64.59, 25.14, 68.59 and 19.20 h at pH 4.0, respectively. Based on the findings, it appeared that the unique polymeric structure of raw OPFL favored cellulases and xylanase productions. Elsevier Ltd. 2019-08-01 Article PeerReviewed Ezeilo, Uchenna R. and Lee, Chew Tin and Huyop, Fahrul and Zakaria, Iffah Izzati and Abdul Wahab, Roswanira (2019) Raw oil palm frond leaves as cost-effective substrate for cellulase and xylanase productions by Trichoderma asperellum UC1 under solid-state fermentation. Journal of Environmental Management, 243 . pp. 206-217. ISSN 0301-4797 http://dx.doi.org/10.1016/j.jenvman.2019.04.113 DOI:10.1016/j.jenvman.2019.04.113 |
| spellingShingle | QH Natural history TP Chemical technology Ezeilo, Uchenna R. Lee, Chew Tin Huyop, Fahrul Zakaria, Iffah Izzati Abdul Wahab, Roswanira Raw oil palm frond leaves as cost-effective substrate for cellulase and xylanase productions by Trichoderma asperellum UC1 under solid-state fermentation |
| title | Raw oil palm frond leaves as cost-effective substrate for cellulase and xylanase productions by Trichoderma asperellum UC1 under solid-state fermentation |
| title_full | Raw oil palm frond leaves as cost-effective substrate for cellulase and xylanase productions by Trichoderma asperellum UC1 under solid-state fermentation |
| title_fullStr | Raw oil palm frond leaves as cost-effective substrate for cellulase and xylanase productions by Trichoderma asperellum UC1 under solid-state fermentation |
| title_full_unstemmed | Raw oil palm frond leaves as cost-effective substrate for cellulase and xylanase productions by Trichoderma asperellum UC1 under solid-state fermentation |
| title_short | Raw oil palm frond leaves as cost-effective substrate for cellulase and xylanase productions by Trichoderma asperellum UC1 under solid-state fermentation |
| title_sort | raw oil palm frond leaves as cost effective substrate for cellulase and xylanase productions by trichoderma asperellum uc1 under solid state fermentation |
| topic | QH Natural history TP Chemical technology |
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