Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering
This work reports the fabrication and characterizations of porous scaffold made up of polylactic acid (PLA) with the inclusion of pectin (1, 3, 5, 7, 9, 11 wt%) for potential tissue engineering material. The composite scaffold was prepared using a facile method of freeze extraction. Based on the phy...
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| Format: | Article |
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De Gruyter
2020
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| _version_ | 1796864539400077312 |
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| author | Hamzah, M. S. A. Razak, S. I. A. Kadir, M. R. A. Bohari, S. P. M. Nayan, N. H. M. Thangaraj Anand, J. S. |
| author_facet | Hamzah, M. S. A. Razak, S. I. A. Kadir, M. R. A. Bohari, S. P. M. Nayan, N. H. M. Thangaraj Anand, J. S. |
| author_sort | Hamzah, M. S. A. |
| collection | ePrints |
| description | This work reports the fabrication and characterizations of porous scaffold made up of polylactic acid (PLA) with the inclusion of pectin (1, 3, 5, 7, 9, 11 wt%) for potential tissue engineering material. The composite scaffold was prepared using a facile method of freeze extraction. Based on the physical evaluations, the scaffold was suggested to be optimum at 5 wt% of pectin loading. Water contact angle of the scaffold was significantly reduced to 46.5o with the inclusion of 5 wt% of pectin. Morphological and topographic of the PLA scaffold revealed that the pectin induced more porous structure and its surface became rougher which was suitable for cell attachment and proliferation. In vitro studies of the PLA/pectin composite scaffold using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidelt (MTT) assay revealed good biocompatibility whereas Live-Dead kit assay resulted in 91% cell viability after 7 days of incubation. |
| first_indexed | 2024-03-05T20:43:24Z |
| format | Article |
| id | utm.eprints-87929 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T20:43:24Z |
| publishDate | 2020 |
| publisher | De Gruyter |
| record_format | dspace |
| spelling | utm.eprints-879292020-11-30T13:37:14Z http://eprints.utm.my/87929/ Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering Hamzah, M. S. A. Razak, S. I. A. Kadir, M. R. A. Bohari, S. P. M. Nayan, N. H. M. Thangaraj Anand, J. S. TP Chemical technology This work reports the fabrication and characterizations of porous scaffold made up of polylactic acid (PLA) with the inclusion of pectin (1, 3, 5, 7, 9, 11 wt%) for potential tissue engineering material. The composite scaffold was prepared using a facile method of freeze extraction. Based on the physical evaluations, the scaffold was suggested to be optimum at 5 wt% of pectin loading. Water contact angle of the scaffold was significantly reduced to 46.5o with the inclusion of 5 wt% of pectin. Morphological and topographic of the PLA scaffold revealed that the pectin induced more porous structure and its surface became rougher which was suitable for cell attachment and proliferation. In vitro studies of the PLA/pectin composite scaffold using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidelt (MTT) assay revealed good biocompatibility whereas Live-Dead kit assay resulted in 91% cell viability after 7 days of incubation. De Gruyter 2020 Article PeerReviewed Hamzah, M. S. A. and Razak, S. I. A. and Kadir, M. R. A. and Bohari, S. P. M. and Nayan, N. H. M. and Thangaraj Anand, J. S. (2020) Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering. Journal of Polymer Engineering, 40 (5). pp. 421-431. ISSN 0334-6447 http://www.dx.doi.org/10.1515/polyeng-2019-0377 DOI: 10.1515/polyeng-2019-0377 |
| spellingShingle | TP Chemical technology Hamzah, M. S. A. Razak, S. I. A. Kadir, M. R. A. Bohari, S. P. M. Nayan, N. H. M. Thangaraj Anand, J. S. Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering |
| title | Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering |
| title_full | Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering |
| title_fullStr | Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering |
| title_full_unstemmed | Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering |
| title_short | Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering |
| title_sort | fabrication and evaluation of polylactic acid pectin composite scaffold via freeze extraction for tissue engineering |
| topic | TP Chemical technology |
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