Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves
Currently, the chemically-assisted esterification to manufacture butyl butyrate employs corrosive homogeneous acid catalyst and liberates enormous quantities of hazardous by-products which complicate downstream treatment processes. This study aimed to identify the optimized esterification conditions...
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| Format: | Article |
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Elsevier Inc.
2019
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| author | Elias, Nursyafiqah Abdul Wahab, Roswanira Chandren, Sheela Abdul Razak, Fazira Ilyana Jamalis, Joazaizulfazli |
| author_facet | Elias, Nursyafiqah Abdul Wahab, Roswanira Chandren, Sheela Abdul Razak, Fazira Ilyana Jamalis, Joazaizulfazli |
| author_sort | Elias, Nursyafiqah |
| collection | ePrints |
| description | Currently, the chemically-assisted esterification to manufacture butyl butyrate employs corrosive homogeneous acid catalyst and liberates enormous quantities of hazardous by-products which complicate downstream treatment processes. This study aimed to identify the optimized esterification conditions, and the kinetic aspects of the enzyme-assisted synthesis of butyl butyrate using immobilized Candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves (CRL/CS-NC). The best process variables that gave the maximum conversion degree of butyl butyrate by CRL/CS-NC (90.2%) in just 3 h, as compared to free CRL (62.9%) are as follows: 50 °C, 1:2 M ratio of acid/alcohol, stirring rate of 200 rpm and a 3 mg/mL enzyme load. The enzymatic esterification followed the ping pong bi-bi mechanism with substrate inhibition, revealing a ˜1.1-fold higher Ki for CRL/CS-NC (55.55 mM) over free CRL (50.68 mM). This indicated that CRL/CS-NC was less inhibited by the substrates. Butanol was preferred over butyric acid as reflected by the higher apparent Michaelis-Menten constant of CRL/CS-NC for butanol (137 mM) than butyric acid (142.7 mM). Thus, the kinetics data conclusively showed that CRL/CS-NC (Vmax 0.48 mM min-1, Keff 0.07 min-1 mM-1) was catalytically more efficient than free CRL (Vmax 0.35 mM min-1, Keff 0.06 min-1 mM-1). |
| first_indexed | 2024-03-05T20:43:38Z |
| format | Article |
| id | utm.eprints-88004 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T20:43:38Z |
| publishDate | 2019 |
| publisher | Elsevier Inc. |
| record_format | dspace |
| spelling | utm.eprints-880042020-12-15T10:41:37Z http://eprints.utm.my/88004/ Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves Elias, Nursyafiqah Abdul Wahab, Roswanira Chandren, Sheela Abdul Razak, Fazira Ilyana Jamalis, Joazaizulfazli QD Chemistry Currently, the chemically-assisted esterification to manufacture butyl butyrate employs corrosive homogeneous acid catalyst and liberates enormous quantities of hazardous by-products which complicate downstream treatment processes. This study aimed to identify the optimized esterification conditions, and the kinetic aspects of the enzyme-assisted synthesis of butyl butyrate using immobilized Candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves (CRL/CS-NC). The best process variables that gave the maximum conversion degree of butyl butyrate by CRL/CS-NC (90.2%) in just 3 h, as compared to free CRL (62.9%) are as follows: 50 °C, 1:2 M ratio of acid/alcohol, stirring rate of 200 rpm and a 3 mg/mL enzyme load. The enzymatic esterification followed the ping pong bi-bi mechanism with substrate inhibition, revealing a ˜1.1-fold higher Ki for CRL/CS-NC (55.55 mM) over free CRL (50.68 mM). This indicated that CRL/CS-NC was less inhibited by the substrates. Butanol was preferred over butyric acid as reflected by the higher apparent Michaelis-Menten constant of CRL/CS-NC for butanol (137 mM) than butyric acid (142.7 mM). Thus, the kinetics data conclusively showed that CRL/CS-NC (Vmax 0.48 mM min-1, Keff 0.07 min-1 mM-1) was catalytically more efficient than free CRL (Vmax 0.35 mM min-1, Keff 0.06 min-1 mM-1). Elsevier Inc. 2019-11 Article PeerReviewed Elias, Nursyafiqah and Abdul Wahab, Roswanira and Chandren, Sheela and Abdul Razak, Fazira Ilyana and Jamalis, Joazaizulfazli (2019) Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves. Enzyme and Microbial Technology, 130 . p. 109367. ISSN 0141-0229 https://dx.doi.org/10.1016/j.enzmictec.2019.109367 |
| spellingShingle | QD Chemistry Elias, Nursyafiqah Abdul Wahab, Roswanira Chandren, Sheela Abdul Razak, Fazira Ilyana Jamalis, Joazaizulfazli Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves |
| title | Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves |
| title_full | Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves |
| title_fullStr | Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves |
| title_full_unstemmed | Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves |
| title_short | Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves |
| title_sort | effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan reinforced nanocellulose derived from raw oil palm leaves |
| topic | QD Chemistry |
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