Production of thermostable pullulanase from Bacillus flavothermus KWF-1: medium development using experimental design with data transformation
The extracellular production of pullulanase from Bacillus flavothermus KWF-1 using experimental design was studied. Two-level full factorial design was applied to enhance these nutrient constituents with respect to pullulanase production. Statistical analysis (ANOVA) have identified that tapioca sta...
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| Format: | Article |
| Language: | English |
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Penerbit UTM Press
2008
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| Online Access: | http://eprints.utm.my/8837/1/UTMjurnalTEK_49F_DIS%5B35%5D.pdf |
| _version_ | 1825910262037741568 |
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| author | Abu Samah, Rozaimi Nik Mahmood, Nik Azmi Goh, Kian Mau Hassan, Osman Md Illias, Rosli |
| author_facet | Abu Samah, Rozaimi Nik Mahmood, Nik Azmi Goh, Kian Mau Hassan, Osman Md Illias, Rosli |
| author_sort | Abu Samah, Rozaimi |
| collection | ePrints |
| description | The extracellular production of pullulanase from Bacillus flavothermus KWF-1 using experimental design was studied. Two-level full factorial design was applied to enhance these nutrient constituents with respect to pullulanase production. Statistical analysis (ANOVA) have identified that tapioca starch was the most significant factor affecting the enzyme production. Diagnostic plots obtained from ANOVA revealed abnormalities with respect to the response data. Therefore, data transformation was advantageous in order to make the distribution of the response variable closer to the normal distribution and to stabilize the variance of the response, as well as to improve the fit of the model to the data. An improved model was attained with higher coefficient of determination (R2 = 0.9523) compared to original R2 of 0.9280. The enhanced medium consisted of (% w/v): 3.0 tapioca starch, 0.11 peptone from casein, 0.08 KH2PO4, 0.02 MnSO4 and an initial pH of 9.0, produced 3.498 U/ml pullulanase, which was approximately 4.5 fold increment than that obtained from initial medium (0.791 U/ml). |
| first_indexed | 2024-03-05T18:14:28Z |
| format | Article |
| id | utm.eprints-8837 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T18:14:28Z |
| publishDate | 2008 |
| publisher | Penerbit UTM Press |
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| spelling | utm.eprints-88372010-10-14T04:43:48Z http://eprints.utm.my/8837/ Production of thermostable pullulanase from Bacillus flavothermus KWF-1: medium development using experimental design with data transformation Abu Samah, Rozaimi Nik Mahmood, Nik Azmi Goh, Kian Mau Hassan, Osman Md Illias, Rosli TP Chemical technology The extracellular production of pullulanase from Bacillus flavothermus KWF-1 using experimental design was studied. Two-level full factorial design was applied to enhance these nutrient constituents with respect to pullulanase production. Statistical analysis (ANOVA) have identified that tapioca starch was the most significant factor affecting the enzyme production. Diagnostic plots obtained from ANOVA revealed abnormalities with respect to the response data. Therefore, data transformation was advantageous in order to make the distribution of the response variable closer to the normal distribution and to stabilize the variance of the response, as well as to improve the fit of the model to the data. An improved model was attained with higher coefficient of determination (R2 = 0.9523) compared to original R2 of 0.9280. The enhanced medium consisted of (% w/v): 3.0 tapioca starch, 0.11 peptone from casein, 0.08 KH2PO4, 0.02 MnSO4 and an initial pH of 9.0, produced 3.498 U/ml pullulanase, which was approximately 4.5 fold increment than that obtained from initial medium (0.791 U/ml). Penerbit UTM Press 2008-12 Article PeerReviewed application/pdf en http://eprints.utm.my/8837/1/UTMjurnalTEK_49F_DIS%5B35%5D.pdf Abu Samah, Rozaimi and Nik Mahmood, Nik Azmi and Goh, Kian Mau and Hassan, Osman and Md Illias, Rosli (2008) Production of thermostable pullulanase from Bacillus flavothermus KWF-1: medium development using experimental design with data transformation. Jurnal Teknologi (49F). pp. 383-396. ISSN 0127-9696 http://www.penerbit.utm.my |
| spellingShingle | TP Chemical technology Abu Samah, Rozaimi Nik Mahmood, Nik Azmi Goh, Kian Mau Hassan, Osman Md Illias, Rosli Production of thermostable pullulanase from Bacillus flavothermus KWF-1: medium development using experimental design with data transformation |
| title | Production of thermostable pullulanase from Bacillus flavothermus KWF-1: medium development using experimental design with data transformation |
| title_full | Production of thermostable pullulanase from Bacillus flavothermus KWF-1: medium development using experimental design with data transformation |
| title_fullStr | Production of thermostable pullulanase from Bacillus flavothermus KWF-1: medium development using experimental design with data transformation |
| title_full_unstemmed | Production of thermostable pullulanase from Bacillus flavothermus KWF-1: medium development using experimental design with data transformation |
| title_short | Production of thermostable pullulanase from Bacillus flavothermus KWF-1: medium development using experimental design with data transformation |
| title_sort | production of thermostable pullulanase from bacillus flavothermus kwf 1 medium development using experimental design with data transformation |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/8837/1/UTMjurnalTEK_49F_DIS%5B35%5D.pdf |
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