Enrichment of Eucalyptus oil nanoemulsion by micellar nanotechnology: transdermal analgesic activity using hot plate test in rats’ assay

Eucalyptus globulus is an aromatic medicinal plant which known for its 1,8-cineole main pharmacological constituent exhibits as natural analgesic agent. Eucalyptus globulus-loaded micellar nanoparticle was developed via spontaneous emulsification technique and further evaluation for its analgesic ef...

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Main Authors: Abdul Aziz, Zarith Asyikin, Mohd. Nasir, Hasmida, Ahmad, Akil, Mohd. Setapar, Siti Hamidah, Ahmad, Hafandi, Mohd. Noor, Mohd. Hezmee, Mohd. Rafatullah, Mohd. Rafatullah, Asma Khatoon, Asma Khatoon, Kausar, Mohd. Adnan, Ahmad, Irfan, Khan, Shahida, Al-Shaeri, Majed, Md. Ashraf, Ghulam
Format: Article
Language:English
Published: Nature Publishing Group 2019
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Online Access:http://eprints.utm.my/89037/1/SitiHamidahMohdSetapar2019_EnrichmentofEucalyptusOilNanoemulsionbyMicellarNanotechnology.pdf
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author Abdul Aziz, Zarith Asyikin
Mohd. Nasir, Hasmida
Ahmad, Akil
Mohd. Setapar, Siti Hamidah
Ahmad, Hafandi
Mohd. Noor, Mohd. Hezmee
Mohd. Rafatullah, Mohd. Rafatullah
Asma Khatoon, Asma Khatoon
Kausar, Mohd. Adnan
Ahmad, Irfan
Khan, Shahida
Al-Shaeri, Majed
Md. Ashraf, Ghulam
author_facet Abdul Aziz, Zarith Asyikin
Mohd. Nasir, Hasmida
Ahmad, Akil
Mohd. Setapar, Siti Hamidah
Ahmad, Hafandi
Mohd. Noor, Mohd. Hezmee
Mohd. Rafatullah, Mohd. Rafatullah
Asma Khatoon, Asma Khatoon
Kausar, Mohd. Adnan
Ahmad, Irfan
Khan, Shahida
Al-Shaeri, Majed
Md. Ashraf, Ghulam
author_sort Abdul Aziz, Zarith Asyikin
collection ePrints
description Eucalyptus globulus is an aromatic medicinal plant which known for its 1,8-cineole main pharmacological constituent exhibits as natural analgesic agent. Eucalyptus globulus-loaded micellar nanoparticle was developed via spontaneous emulsification technique and further evaluation for its analgesic efficacy study, in vivo analgesic activity assay in rats. The nanoemulsion system containing Eucalyptus-micelles was optimized at different surfactant types (Tween 40, 60 and 80) and concentrations (3.0, 6.0, 9.0, 12.0, 15.0, and 18.0 wt. %). These formulations were characterized by thermodynamically stability, viscosity, micelles particle size, pH, and morphology structure. The spontaneous emulsification technique offered a greener micelles formation in nanoemulsion system by slowly titrated of organic phase, containing Eucalyptus globulus (active compound), grape seed oil (carrier oil) and hydrophilic surfactant into aqueous phase, and continuously stirred for 30 min to form a homogeneity solution. The characterizations evaluation revealed an optimized formulation with Tween 40 surfactant type at 9.0 wt. % of surfactant concentration promoted the most thermodynamic stability, smaller micelles particle size (d = 17.13 ± 0.035 nm) formed with spherical shape morphological structure, and suitable in viscosity (≈2.3 cP) and pH value (6.57) for transdermal purpose. The in vivo analgesic activity assay of optimized emulsion showed that the transdermal administration of micellar nanoparticle of Eucalyptus globulus on fore and hind limb of rats, possessed the central and peripheral analgesic effects by prolonged the rats pain responses towards the heat stimulus after being put on top of hot plate (55 °C), with longest time responses, 40.75 s at 60 min after treatment administration. Thus, this study demonstrated that micellar nanoparticle of Eucalyptus globulus formed in nanoemulsion system could be promising as an efficient transdermal nanocarrier for the analgesic therapy alternative.
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spelling utm.eprints-890372021-01-26T08:41:42Z http://eprints.utm.my/89037/ Enrichment of Eucalyptus oil nanoemulsion by micellar nanotechnology: transdermal analgesic activity using hot plate test in rats’ assay Abdul Aziz, Zarith Asyikin Mohd. Nasir, Hasmida Ahmad, Akil Mohd. Setapar, Siti Hamidah Ahmad, Hafandi Mohd. Noor, Mohd. Hezmee Mohd. Rafatullah, Mohd. Rafatullah Asma Khatoon, Asma Khatoon Kausar, Mohd. Adnan Ahmad, Irfan Khan, Shahida Al-Shaeri, Majed Md. Ashraf, Ghulam QD Chemistry Eucalyptus globulus is an aromatic medicinal plant which known for its 1,8-cineole main pharmacological constituent exhibits as natural analgesic agent. Eucalyptus globulus-loaded micellar nanoparticle was developed via spontaneous emulsification technique and further evaluation for its analgesic efficacy study, in vivo analgesic activity assay in rats. The nanoemulsion system containing Eucalyptus-micelles was optimized at different surfactant types (Tween 40, 60 and 80) and concentrations (3.0, 6.0, 9.0, 12.0, 15.0, and 18.0 wt. %). These formulations were characterized by thermodynamically stability, viscosity, micelles particle size, pH, and morphology structure. The spontaneous emulsification technique offered a greener micelles formation in nanoemulsion system by slowly titrated of organic phase, containing Eucalyptus globulus (active compound), grape seed oil (carrier oil) and hydrophilic surfactant into aqueous phase, and continuously stirred for 30 min to form a homogeneity solution. The characterizations evaluation revealed an optimized formulation with Tween 40 surfactant type at 9.0 wt. % of surfactant concentration promoted the most thermodynamic stability, smaller micelles particle size (d = 17.13 ± 0.035 nm) formed with spherical shape morphological structure, and suitable in viscosity (≈2.3 cP) and pH value (6.57) for transdermal purpose. The in vivo analgesic activity assay of optimized emulsion showed that the transdermal administration of micellar nanoparticle of Eucalyptus globulus on fore and hind limb of rats, possessed the central and peripheral analgesic effects by prolonged the rats pain responses towards the heat stimulus after being put on top of hot plate (55 °C), with longest time responses, 40.75 s at 60 min after treatment administration. Thus, this study demonstrated that micellar nanoparticle of Eucalyptus globulus formed in nanoemulsion system could be promising as an efficient transdermal nanocarrier for the analgesic therapy alternative. Nature Publishing Group 2019-12 Article PeerReviewed application/pdf en http://eprints.utm.my/89037/1/SitiHamidahMohdSetapar2019_EnrichmentofEucalyptusOilNanoemulsionbyMicellarNanotechnology.pdf Abdul Aziz, Zarith Asyikin and Mohd. Nasir, Hasmida and Ahmad, Akil and Mohd. Setapar, Siti Hamidah and Ahmad, Hafandi and Mohd. Noor, Mohd. Hezmee and Mohd. Rafatullah, Mohd. Rafatullah and Asma Khatoon, Asma Khatoon and Kausar, Mohd. Adnan and Ahmad, Irfan and Khan, Shahida and Al-Shaeri, Majed and Md. Ashraf, Ghulam (2019) Enrichment of Eucalyptus oil nanoemulsion by micellar nanotechnology: transdermal analgesic activity using hot plate test in rats’ assay. Scientific Reports, 9 (1). p. 13678. ISSN 2045-2322 http://dx.doi.org/10.1038/s41598-019-50134-y
spellingShingle QD Chemistry
Abdul Aziz, Zarith Asyikin
Mohd. Nasir, Hasmida
Ahmad, Akil
Mohd. Setapar, Siti Hamidah
Ahmad, Hafandi
Mohd. Noor, Mohd. Hezmee
Mohd. Rafatullah, Mohd. Rafatullah
Asma Khatoon, Asma Khatoon
Kausar, Mohd. Adnan
Ahmad, Irfan
Khan, Shahida
Al-Shaeri, Majed
Md. Ashraf, Ghulam
Enrichment of Eucalyptus oil nanoemulsion by micellar nanotechnology: transdermal analgesic activity using hot plate test in rats’ assay
title Enrichment of Eucalyptus oil nanoemulsion by micellar nanotechnology: transdermal analgesic activity using hot plate test in rats’ assay
title_full Enrichment of Eucalyptus oil nanoemulsion by micellar nanotechnology: transdermal analgesic activity using hot plate test in rats’ assay
title_fullStr Enrichment of Eucalyptus oil nanoemulsion by micellar nanotechnology: transdermal analgesic activity using hot plate test in rats’ assay
title_full_unstemmed Enrichment of Eucalyptus oil nanoemulsion by micellar nanotechnology: transdermal analgesic activity using hot plate test in rats’ assay
title_short Enrichment of Eucalyptus oil nanoemulsion by micellar nanotechnology: transdermal analgesic activity using hot plate test in rats’ assay
title_sort enrichment of eucalyptus oil nanoemulsion by micellar nanotechnology transdermal analgesic activity using hot plate test in rats assay
topic QD Chemistry
url http://eprints.utm.my/89037/1/SitiHamidahMohdSetapar2019_EnrichmentofEucalyptusOilNanoemulsionbyMicellarNanotechnology.pdf
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