Determination of Chromium(VI) reduction by acinetobacter haemolyticus using x-ray absorption fine structure spectroscopy
Several types of microorganisms have been reported to reduce Cr(VI) to the less toxic Cr(III) via enzymatic reactions. The main purpose of this study is to determine the reduction of Cr(VI) by a locally isolated bacterium, Acinetobacter haemolyticus (A. haemolyticus) using the x-ray absorption fine...
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| Format: | Article |
| Language: | English |
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Ibnu Sina Institute for Fundamental Science Studies, Universiti Teknologi Malaysia
2008
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| Online Access: | http://eprints.utm.my/8974/1/ShafinazShahir2008_DeterminationofChromium.pdf |
| _version_ | 1796854469212766208 |
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| author | Hsiao, Pei Quek Shahir, Shafinaz Liu, Tao Ahmad, Wan Azlina |
| author_facet | Hsiao, Pei Quek Shahir, Shafinaz Liu, Tao Ahmad, Wan Azlina |
| author_sort | Hsiao, Pei Quek |
| collection | ePrints |
| description | Several types of microorganisms have been reported to reduce Cr(VI) to the less toxic Cr(III) via enzymatic reactions. The main purpose of this study is to determine the reduction of Cr(VI) by a locally isolated bacterium, Acinetobacter haemolyticus (A. haemolyticus) using the x-ray absorption fine structure (XAFS) spectroscopy. XAFS analysis consisted of both the x-ray absorption near-edge structure (XANES) and extended x-ray absorption fine structure (EXAFS) spectra. XANES spectra of the rinsed Cr(VI)-laden A. haemolyticus biomass indicated that chromium was only present in the form of Cr (III). The nearest atoms coordinated to Cr(III) were oxygens in an octahedral geometry. The longer Cr-O bond lengths (~1.97 Å) obtained from the EXAFS spectra corroborated the presence of chromium as Cr(III) bonded to oxygen. These findings demonstrate the ability of A. haemolyticus to reduce Cr(VI) to Cr(III) that was bound to oxygen atoms of the ligands in A. haemolyticus |
| first_indexed | 2024-03-05T18:14:32Z |
| format | Article |
| id | utm.eprints-8974 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T18:14:32Z |
| publishDate | 2008 |
| publisher | Ibnu Sina Institute for Fundamental Science Studies, Universiti Teknologi Malaysia |
| record_format | dspace |
| spelling | utm.eprints-89742010-10-20T08:43:15Z http://eprints.utm.my/8974/ Determination of Chromium(VI) reduction by acinetobacter haemolyticus using x-ray absorption fine structure spectroscopy Hsiao, Pei Quek Shahir, Shafinaz Liu, Tao Ahmad, Wan Azlina QD Chemistry Several types of microorganisms have been reported to reduce Cr(VI) to the less toxic Cr(III) via enzymatic reactions. The main purpose of this study is to determine the reduction of Cr(VI) by a locally isolated bacterium, Acinetobacter haemolyticus (A. haemolyticus) using the x-ray absorption fine structure (XAFS) spectroscopy. XAFS analysis consisted of both the x-ray absorption near-edge structure (XANES) and extended x-ray absorption fine structure (EXAFS) spectra. XANES spectra of the rinsed Cr(VI)-laden A. haemolyticus biomass indicated that chromium was only present in the form of Cr (III). The nearest atoms coordinated to Cr(III) were oxygens in an octahedral geometry. The longer Cr-O bond lengths (~1.97 Å) obtained from the EXAFS spectra corroborated the presence of chromium as Cr(III) bonded to oxygen. These findings demonstrate the ability of A. haemolyticus to reduce Cr(VI) to Cr(III) that was bound to oxygen atoms of the ligands in A. haemolyticus Ibnu Sina Institute for Fundamental Science Studies, Universiti Teknologi Malaysia 2008 Article PeerReviewed application/pdf en http://eprints.utm.my/8974/1/ShafinazShahir2008_DeterminationofChromium.pdf Hsiao, Pei Quek and Shahir, Shafinaz and Liu, Tao and Ahmad, Wan Azlina (2008) Determination of Chromium(VI) reduction by acinetobacter haemolyticus using x-ray absorption fine structure spectroscopy. Journal of Fundamental Sciences, 4 (2). pp. 415-422. ISSN 1823-626X http://jfs.ibnusina.utm.my/index.php/jfs/article/view/70 |
| spellingShingle | QD Chemistry Hsiao, Pei Quek Shahir, Shafinaz Liu, Tao Ahmad, Wan Azlina Determination of Chromium(VI) reduction by acinetobacter haemolyticus using x-ray absorption fine structure spectroscopy |
| title | Determination of Chromium(VI) reduction by acinetobacter haemolyticus using x-ray absorption fine structure spectroscopy |
| title_full | Determination of Chromium(VI) reduction by acinetobacter haemolyticus using x-ray absorption fine structure spectroscopy |
| title_fullStr | Determination of Chromium(VI) reduction by acinetobacter haemolyticus using x-ray absorption fine structure spectroscopy |
| title_full_unstemmed | Determination of Chromium(VI) reduction by acinetobacter haemolyticus using x-ray absorption fine structure spectroscopy |
| title_short | Determination of Chromium(VI) reduction by acinetobacter haemolyticus using x-ray absorption fine structure spectroscopy |
| title_sort | determination of chromium vi reduction by acinetobacter haemolyticus using x ray absorption fine structure spectroscopy |
| topic | QD Chemistry |
| url | http://eprints.utm.my/8974/1/ShafinazShahir2008_DeterminationofChromium.pdf |
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