Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli
Cell surface display of xylanase on Escherichia coli was used for the hydrolysis of hemicellulose from pineapple pomace. The feasibility of bioconversion of lignocellulosic biomass into xylooligosaccharides (XOS) was investigated. In this study, pineapple pomace was pretreated, and the hemicellulose...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Published: |
Springer Science and Business Media Deutschland GmbH
2020
|
Subjects: |
_version_ | 1796865108730707968 |
---|---|
author | Wee, Mei Yuin Joanne Wan Azelee, Nur Izyan Pachelles, Samson Abd. Murad, Abdul Munir Abu Bakar, Farah Diba Md. Illias, Rosli |
author_facet | Wee, Mei Yuin Joanne Wan Azelee, Nur Izyan Pachelles, Samson Abd. Murad, Abdul Munir Abu Bakar, Farah Diba Md. Illias, Rosli |
author_sort | Wee, Mei Yuin Joanne |
collection | ePrints |
description | Cell surface display of xylanase on Escherichia coli was used for the hydrolysis of hemicellulose from pineapple pomace. The feasibility of bioconversion of lignocellulosic biomass into xylooligosaccharides (XOS) was investigated. In this study, pineapple pomace was pretreated, and the hemicellulose fraction was obtained for reaction with the whole-cell biocatalyst. FESEM and FTIR analyses were used to observe morphological and compositional changes of pineapple pomace respectively after pretreatment. Factors affecting hydrolysis reaction were investigated and optimized using the Box-Behnken Design. The highest amount of reducing sugar was produced at pH 7.5, cell loading of 100 g/L wet cell weight, and temperature of 30 °C. The amount of reducing sugar produced was 2.129 mg/ml. HPLC analysis indicated that the XOS produced were xylobiose and xylotriose with a total yield of 5.4 mg/g of pineapple hemicellulose. FESEM analysis on the surface structure of pineapple pomace after the hydrolysis reaction showed clear signs of degradation by xylanase. Based on the results presented, it can be deduced that the application of cell surface display on E. coli for degradation of lignocellulosic biomass is possible and should be explored as it offers great potential for the production of XOS in industry.L |
first_indexed | 2024-03-05T20:51:57Z |
format | Article |
id | utm.eprints-90834 |
institution | Universiti Teknologi Malaysia - ePrints |
last_indexed | 2024-03-05T20:51:57Z |
publishDate | 2020 |
publisher | Springer Science and Business Media Deutschland GmbH |
record_format | dspace |
spelling | utm.eprints-908342021-05-31T13:21:57Z http://eprints.utm.my/90834/ Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli Wee, Mei Yuin Joanne Wan Azelee, Nur Izyan Pachelles, Samson Abd. Murad, Abdul Munir Abu Bakar, Farah Diba Md. Illias, Rosli TP Chemical technology Cell surface display of xylanase on Escherichia coli was used for the hydrolysis of hemicellulose from pineapple pomace. The feasibility of bioconversion of lignocellulosic biomass into xylooligosaccharides (XOS) was investigated. In this study, pineapple pomace was pretreated, and the hemicellulose fraction was obtained for reaction with the whole-cell biocatalyst. FESEM and FTIR analyses were used to observe morphological and compositional changes of pineapple pomace respectively after pretreatment. Factors affecting hydrolysis reaction were investigated and optimized using the Box-Behnken Design. The highest amount of reducing sugar was produced at pH 7.5, cell loading of 100 g/L wet cell weight, and temperature of 30 °C. The amount of reducing sugar produced was 2.129 mg/ml. HPLC analysis indicated that the XOS produced were xylobiose and xylotriose with a total yield of 5.4 mg/g of pineapple hemicellulose. FESEM analysis on the surface structure of pineapple pomace after the hydrolysis reaction showed clear signs of degradation by xylanase. Based on the results presented, it can be deduced that the application of cell surface display on E. coli for degradation of lignocellulosic biomass is possible and should be explored as it offers great potential for the production of XOS in industry.L Springer Science and Business Media Deutschland GmbH 2020 Article PeerReviewed Wee, Mei Yuin Joanne and Wan Azelee, Nur Izyan and Pachelles, Samson and Abd. Murad, Abdul Munir and Abu Bakar, Farah Diba and Md. Illias, Rosli (2020) Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli. Biomass Conversion and Biorefinery . ISSN 2190-6815 http://dx.doi.org/10.1007/s13399-020-01041-0 |
spellingShingle | TP Chemical technology Wee, Mei Yuin Joanne Wan Azelee, Nur Izyan Pachelles, Samson Abd. Murad, Abdul Munir Abu Bakar, Farah Diba Md. Illias, Rosli Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli |
title | Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli |
title_full | Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli |
title_fullStr | Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli |
title_full_unstemmed | Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli |
title_short | Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli |
title_sort | bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on escherichia coli |
topic | TP Chemical technology |
work_keys_str_mv | AT weemeiyuinjoanne bioconversionofpineapplepomaceforxylooligosaccharidesynthesisusingsurfacedisplayofxylanaseonescherichiacoli AT wanazeleenurizyan bioconversionofpineapplepomaceforxylooligosaccharidesynthesisusingsurfacedisplayofxylanaseonescherichiacoli AT pachellessamson bioconversionofpineapplepomaceforxylooligosaccharidesynthesisusingsurfacedisplayofxylanaseonescherichiacoli AT abdmuradabdulmunir bioconversionofpineapplepomaceforxylooligosaccharidesynthesisusingsurfacedisplayofxylanaseonescherichiacoli AT abubakarfarahdiba bioconversionofpineapplepomaceforxylooligosaccharidesynthesisusingsurfacedisplayofxylanaseonescherichiacoli AT mdilliasrosli bioconversionofpineapplepomaceforxylooligosaccharidesynthesisusingsurfacedisplayofxylanaseonescherichiacoli |