Developing herbicide tolerant transgenic plants for sustainable weed management
The use of herbicide is important in the modern integrated weed management system. However, herbicide also can kill valuable crops and cause significant losses in agricultural activity. One of the solutions to this problem is by developing herbicide resistant plant for instance using dehD gene from...
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| Format: | Article |
| Language: | English |
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World Academy of Research in Science and Engi
2020
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| Online Access: | http://eprints.utm.my/90973/1/FahrulZamanHuyop2020_DevelopingHerbicideTolerantTransgenicPlants.pdf |
| _version_ | 1796865136016752640 |
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| author | Mohamed, Elizah Mohamed, Johar Huyop, Fahrul Abdul Wahab, Roswanira Abdul Hamid, Hazandy |
| author_facet | Mohamed, Elizah Mohamed, Johar Huyop, Fahrul Abdul Wahab, Roswanira Abdul Hamid, Hazandy |
| author_sort | Mohamed, Elizah |
| collection | ePrints |
| description | The use of herbicide is important in the modern integrated weed management system. However, herbicide also can kill valuable crops and cause significant losses in agricultural activity. One of the solutions to this problem is by developing herbicide resistant plant for instance using dehD gene from Rhizobial system. In this study, we developed transgenic tobacco plants that were resistant to monochloroacetic acid (MCA) herbicide. A gene from Rhizobium sp. coding for a dehalogenase D (dehD) capable of degrading monochloroacetic acid (MCA) has been previously isolated, characterised, cloned and sequenced. The coding sequence was fused with a cauliflower mosaic virus 35S promoter and introduced into tobacco plants by Agrobacterium-mediated gene transfer. In plant transformation experiments, the gene was shown to confer tolerance to MCA at a tissue culture level where MCA could be used to select for transformants. Integration and expression of the dehD gene in regenerated plants was confirmed by PCR analyses and Reverse Transcriptase PCR, respectively. The Chi Square analyses of five transgenic plants (T1), suggested that the dehD gene was segregated according to Mendelian 3:1 ratio. These findings showed that transgenic N. benthamiana plant resistant to MCA herbicide was successfully produced. The mode of action of dehD enzyme is not known but they probably affect many enzyme pathways. dehD gene has several advantages as a marker in plant breeding and genetic studies. In our knowledge, this is the first experimental data of transgenic N. benthamiana engineered with dehD gene originated Rhizobium sp. |
| first_indexed | 2024-03-05T20:52:21Z |
| format | Article |
| id | utm.eprints-90973 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T20:52:21Z |
| publishDate | 2020 |
| publisher | World Academy of Research in Science and Engi |
| record_format | dspace |
| spelling | utm.eprints-909732021-05-31T13:28:56Z http://eprints.utm.my/90973/ Developing herbicide tolerant transgenic plants for sustainable weed management Mohamed, Elizah Mohamed, Johar Huyop, Fahrul Abdul Wahab, Roswanira Abdul Hamid, Hazandy QD Chemistry The use of herbicide is important in the modern integrated weed management system. However, herbicide also can kill valuable crops and cause significant losses in agricultural activity. One of the solutions to this problem is by developing herbicide resistant plant for instance using dehD gene from Rhizobial system. In this study, we developed transgenic tobacco plants that were resistant to monochloroacetic acid (MCA) herbicide. A gene from Rhizobium sp. coding for a dehalogenase D (dehD) capable of degrading monochloroacetic acid (MCA) has been previously isolated, characterised, cloned and sequenced. The coding sequence was fused with a cauliflower mosaic virus 35S promoter and introduced into tobacco plants by Agrobacterium-mediated gene transfer. In plant transformation experiments, the gene was shown to confer tolerance to MCA at a tissue culture level where MCA could be used to select for transformants. Integration and expression of the dehD gene in regenerated plants was confirmed by PCR analyses and Reverse Transcriptase PCR, respectively. The Chi Square analyses of five transgenic plants (T1), suggested that the dehD gene was segregated according to Mendelian 3:1 ratio. These findings showed that transgenic N. benthamiana plant resistant to MCA herbicide was successfully produced. The mode of action of dehD enzyme is not known but they probably affect many enzyme pathways. dehD gene has several advantages as a marker in plant breeding and genetic studies. In our knowledge, this is the first experimental data of transgenic N. benthamiana engineered with dehD gene originated Rhizobium sp. World Academy of Research in Science and Engi 2020 Article PeerReviewed application/pdf en http://eprints.utm.my/90973/1/FahrulZamanHuyop2020_DevelopingHerbicideTolerantTransgenicPlants.pdf Mohamed, Elizah and Mohamed, Johar and Huyop, Fahrul and Abdul Wahab, Roswanira and Abdul Hamid, Hazandy (2020) Developing herbicide tolerant transgenic plants for sustainable weed management. International Journal of Emerging Trends in Engineering Research, 8 (1 1.2). pp. 60-66. ISSN 2347-3983 http://dx.doi.org/10.30534/ijeter/2020/0981.22020 |
| spellingShingle | QD Chemistry Mohamed, Elizah Mohamed, Johar Huyop, Fahrul Abdul Wahab, Roswanira Abdul Hamid, Hazandy Developing herbicide tolerant transgenic plants for sustainable weed management |
| title | Developing herbicide tolerant transgenic plants for
sustainable weed management |
| title_full | Developing herbicide tolerant transgenic plants for
sustainable weed management |
| title_fullStr | Developing herbicide tolerant transgenic plants for
sustainable weed management |
| title_full_unstemmed | Developing herbicide tolerant transgenic plants for
sustainable weed management |
| title_short | Developing herbicide tolerant transgenic plants for
sustainable weed management |
| title_sort | developing herbicide tolerant transgenic plants for sustainable weed management |
| topic | QD Chemistry |
| url | http://eprints.utm.my/90973/1/FahrulZamanHuyop2020_DevelopingHerbicideTolerantTransgenicPlants.pdf |
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