Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation
The substrate plays an important role in solid state fermentation (SSF) in the production of exo-polygalacturonase (one type of the pectinase enzymes). The aim of this study was to investigate a pretreatment method on Nephrolepis biserrata leaves as a substrate for the production of exo-polygalactur...
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Foundation for Enviromental Protection and Research
2019
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| _version_ | 1796865233180950528 |
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| author | Pagarra, H. Rahman, R. A. Rachmawaty, Rachmawaty |
| author_facet | Pagarra, H. Rahman, R. A. Rachmawaty, Rachmawaty |
| author_sort | Pagarra, H. |
| collection | ePrints |
| description | The substrate plays an important role in solid state fermentation (SSF) in the production of exo-polygalacturonase (one type of the pectinase enzymes). The aim of this study was to investigate a pretreatment method on Nephrolepis biserrata leaves as a substrate for the production of exo-polygalacturonase. The pretreatment steps improved the decomposition of the lignocellulosic material in the structure of N. biserrata leaves as a substrate and allowed Aspergillus niger better access of the substrate to the enzymatic reaction. This optimised both exo-polygalacturonase activity and the growth of A. niger in SSF. Pretreatment was performed under alkali, acidic, autohydrolytic and control conditions. The highest exo-polygalacturonase activity was 39.16 U/g and biomass of A. niger was 0.39 mg/gdsf at 120 h was found for the autohydrolysis pretreatment, as compared to alkali pretreatment, acid pretreatment and control. Scanning electron microscope images showed a marked change in the physical appearance of treated N. biserrata leaves as compared to untreated N. biserrata leaves, especially following autohydrolysis pretreatment. |
| first_indexed | 2024-03-05T20:53:50Z |
| format | Article |
| id | utm.eprints-91467 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T20:53:50Z |
| publishDate | 2019 |
| publisher | Foundation for Enviromental Protection and Research |
| record_format | dspace |
| spelling | utm.eprints-914672021-06-30T12:16:35Z http://eprints.utm.my/91467/ Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation Pagarra, H. Rahman, R. A. Rachmawaty, Rachmawaty TP Chemical technology The substrate plays an important role in solid state fermentation (SSF) in the production of exo-polygalacturonase (one type of the pectinase enzymes). The aim of this study was to investigate a pretreatment method on Nephrolepis biserrata leaves as a substrate for the production of exo-polygalacturonase. The pretreatment steps improved the decomposition of the lignocellulosic material in the structure of N. biserrata leaves as a substrate and allowed Aspergillus niger better access of the substrate to the enzymatic reaction. This optimised both exo-polygalacturonase activity and the growth of A. niger in SSF. Pretreatment was performed under alkali, acidic, autohydrolytic and control conditions. The highest exo-polygalacturonase activity was 39.16 U/g and biomass of A. niger was 0.39 mg/gdsf at 120 h was found for the autohydrolysis pretreatment, as compared to alkali pretreatment, acid pretreatment and control. Scanning electron microscope images showed a marked change in the physical appearance of treated N. biserrata leaves as compared to untreated N. biserrata leaves, especially following autohydrolysis pretreatment. Foundation for Enviromental Protection and Research 2019 Article PeerReviewed Pagarra, H. and Rahman, R. A. and Rachmawaty, Rachmawaty (2019) Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation. EurAsian Journal of BioSciences, 13 (1). pp. 199-206. ISSN 1307-9867 http://www.ejobios.org/download/biochemical-study-of-the-effect-of-nephrolepis-biserrata-pretreatment-on-exo-polygalacturonase-6193.pdf. |
| spellingShingle | TP Chemical technology Pagarra, H. Rahman, R. A. Rachmawaty, Rachmawaty Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation |
| title | Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation |
| title_full | Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation |
| title_fullStr | Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation |
| title_full_unstemmed | Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation |
| title_short | Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation |
| title_sort | biochemical study of the effect of nephrolepis biserrata pretreatment on exo polygalacturonase production by aspergilllus niger in solid state fermentation |
| topic | TP Chemical technology |
| work_keys_str_mv | AT pagarrah biochemicalstudyoftheeffectofnephrolepisbiserratapretreatmentonexopolygalacturonaseproductionbyaspergilllusnigerinsolidstatefermentation AT rahmanra biochemicalstudyoftheeffectofnephrolepisbiserratapretreatmentonexopolygalacturonaseproductionbyaspergilllusnigerinsolidstatefermentation AT rachmawatyrachmawaty biochemicalstudyoftheeffectofnephrolepisbiserratapretreatmentonexopolygalacturonaseproductionbyaspergilllusnigerinsolidstatefermentation |