Development and in vitro evaluation of κ-carrageenan based polymeric hybrid nanocomposite scaffolds for bone tissue engineering
The excellent biocompatible and osteogenesis characteristics of porous scaffolds play a vital role in bone regeneration. In this study, we have synthesized polymeric hybrid nanocomposites via free-radical polymerization from carrageenan/acrylic-acid/graphene/hydroxyapatite. Porous hybrid nanocomposi...
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| Format: | Article |
| Language: | English |
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Royal Society of Chemistry
2020
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| Online Access: | http://eprints.utm.my/93399/1/MohammedRafiq2020_DevelopmentandinvitroevaluationofKcarrageenan.pdf |
| _version_ | 1796865613956644864 |
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| author | Aslam Khan, Muhammad Umar Ali Raza, Mohsin Mehboob, Hassan Abdul Kadir, Mohammed Rafiq Abd. Razak, Saiful Izwan A. Shah, Saqlain Iqbal, Muhammad Zahir Amin, Rashid |
| author_facet | Aslam Khan, Muhammad Umar Ali Raza, Mohsin Mehboob, Hassan Abdul Kadir, Mohammed Rafiq Abd. Razak, Saiful Izwan A. Shah, Saqlain Iqbal, Muhammad Zahir Amin, Rashid |
| author_sort | Aslam Khan, Muhammad Umar |
| collection | ePrints |
| description | The excellent biocompatible and osteogenesis characteristics of porous scaffolds play a vital role in bone regeneration. In this study, we have synthesized polymeric hybrid nanocomposites via free-radical polymerization from carrageenan/acrylic-acid/graphene/hydroxyapatite. Porous hybrid nanocomposite scaffolds were fabricated through a freeze-drying method to mimic the structural and chemical composition of natural bone. Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and water contact-angle studies were carried-out for functional groups, surface morphology and hydrophilicity of the materials, followed by biodegradation and swelling analysis. The cell viability, cell culture and proliferation were evaluated against mouse pre-osteoblast (MC3T3-E1) cell lines using neutral red dye assay. The cell adherence and proliferation studies were determined by SEM. Physical characterization including optimum porosity and pore size (49.75% and 0.41 × 103 μm2), mechanical properties (compression strength 8.87 MPa and elastic modulus 442.63 MPa), swelling (70.20% at 27 °C and 77.21% at 37 °C) and biodegradation (23.8%) were performed. The results indicated CG-g-AAc-3 with a high optical density and better cell viability. Hence, CG-g-AAc-3 was found to be more efficient for bone regeneration with potential applications in fractured bone regeneration. |
| first_indexed | 2024-03-05T20:59:41Z |
| format | Article |
| id | utm.eprints-93399 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T20:59:41Z |
| publishDate | 2020 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | utm.eprints-933992021-11-30T08:28:51Z http://eprints.utm.my/93399/ Development and in vitro evaluation of κ-carrageenan based polymeric hybrid nanocomposite scaffolds for bone tissue engineering Aslam Khan, Muhammad Umar Ali Raza, Mohsin Mehboob, Hassan Abdul Kadir, Mohammed Rafiq Abd. Razak, Saiful Izwan A. Shah, Saqlain Iqbal, Muhammad Zahir Amin, Rashid Q Science (General) The excellent biocompatible and osteogenesis characteristics of porous scaffolds play a vital role in bone regeneration. In this study, we have synthesized polymeric hybrid nanocomposites via free-radical polymerization from carrageenan/acrylic-acid/graphene/hydroxyapatite. Porous hybrid nanocomposite scaffolds were fabricated through a freeze-drying method to mimic the structural and chemical composition of natural bone. Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and water contact-angle studies were carried-out for functional groups, surface morphology and hydrophilicity of the materials, followed by biodegradation and swelling analysis. The cell viability, cell culture and proliferation were evaluated against mouse pre-osteoblast (MC3T3-E1) cell lines using neutral red dye assay. The cell adherence and proliferation studies were determined by SEM. Physical characterization including optimum porosity and pore size (49.75% and 0.41 × 103 μm2), mechanical properties (compression strength 8.87 MPa and elastic modulus 442.63 MPa), swelling (70.20% at 27 °C and 77.21% at 37 °C) and biodegradation (23.8%) were performed. The results indicated CG-g-AAc-3 with a high optical density and better cell viability. Hence, CG-g-AAc-3 was found to be more efficient for bone regeneration with potential applications in fractured bone regeneration. Royal Society of Chemistry 2020 Article PeerReviewed application/pdf en http://eprints.utm.my/93399/1/MohammedRafiq2020_DevelopmentandinvitroevaluationofKcarrageenan.pdf Aslam Khan, Muhammad Umar and Ali Raza, Mohsin and Mehboob, Hassan and Abdul Kadir, Mohammed Rafiq and Abd. Razak, Saiful Izwan and A. Shah, Saqlain and Iqbal, Muhammad Zahir and Amin, Rashid (2020) Development and in vitro evaluation of κ-carrageenan based polymeric hybrid nanocomposite scaffolds for bone tissue engineering. RSC Advances, 10 (66). pp. 40529-40542. ISSN 2046-2069 http://dx.doi.org/10.1039/d0ra07446b |
| spellingShingle | Q Science (General) Aslam Khan, Muhammad Umar Ali Raza, Mohsin Mehboob, Hassan Abdul Kadir, Mohammed Rafiq Abd. Razak, Saiful Izwan A. Shah, Saqlain Iqbal, Muhammad Zahir Amin, Rashid Development and in vitro evaluation of κ-carrageenan based polymeric hybrid nanocomposite scaffolds for bone tissue engineering |
| title | Development and in vitro evaluation of κ-carrageenan based polymeric hybrid nanocomposite scaffolds for bone tissue engineering |
| title_full | Development and in vitro evaluation of κ-carrageenan based polymeric hybrid nanocomposite scaffolds for bone tissue engineering |
| title_fullStr | Development and in vitro evaluation of κ-carrageenan based polymeric hybrid nanocomposite scaffolds for bone tissue engineering |
| title_full_unstemmed | Development and in vitro evaluation of κ-carrageenan based polymeric hybrid nanocomposite scaffolds for bone tissue engineering |
| title_short | Development and in vitro evaluation of κ-carrageenan based polymeric hybrid nanocomposite scaffolds for bone tissue engineering |
| title_sort | development and in vitro evaluation of κ carrageenan based polymeric hybrid nanocomposite scaffolds for bone tissue engineering |
| topic | Q Science (General) |
| url | http://eprints.utm.my/93399/1/MohammedRafiq2020_DevelopmentandinvitroevaluationofKcarrageenan.pdf |
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