Evaluation of protein content and antioxidant activity of edible bird's nest by various methods

The aim of this study was to evaluate the protein content and antioxidant activity of instant and raw Edible bird’s nest (EBN). This study was conducted using two types of EBNs, which are instant and raw samples. All EBN samples were extracted via three types of extraction method, namely, salt, alka...

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Main Authors: Zamri, S. S., Mahadi, M., Abdullah, F., Syafiuddin, A., Hadibarata, T.
Format: Article
Language:English
Published: AMG Transcend Association 2020
Subjects:
Online Access:http://eprints.utm.my/93614/1/FaizuanAbdullah2020_EvaluationofProteinContent.pdf
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author Zamri, S. S.
Mahadi, M.
Abdullah, F.
Syafiuddin, A.
Hadibarata, T.
author_facet Zamri, S. S.
Mahadi, M.
Abdullah, F.
Syafiuddin, A.
Hadibarata, T.
author_sort Zamri, S. S.
collection ePrints
description The aim of this study was to evaluate the protein content and antioxidant activity of instant and raw Edible bird’s nest (EBN). This study was conducted using two types of EBNs, which are instant and raw samples. All EBN samples were extracted via three types of extraction method, namely, salt, alkaline, and hot extractions. Lowry’s method was used to analyze protein content and the antioxidant activities were analyzed via free radical scavenging assay 1,1-diphenyl-2-pycrylhydrazyl (DPPH), total phenolic content (TPC) assay and ferric reducing antioxidant power (FRAP). This study showed that the highest protein was 2165.90 µg/mL obtained from raw EBN extracted via alkaline solution. In addition, it was also found that the protein concentration of raw EBN was higher compared to instant EBN for all types of extraction procedures. Results from antioxidant assay showed that there was no significant difference between DPPH and FRAP of both EBN. Moreover, TPC results showed that the there was no phenolic compounds detected via all extraction procedures.
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spelling utm.eprints-936142021-12-31T07:34:36Z http://eprints.utm.my/93614/ Evaluation of protein content and antioxidant activity of edible bird's nest by various methods Zamri, S. S. Mahadi, M. Abdullah, F. Syafiuddin, A. Hadibarata, T. QD Chemistry The aim of this study was to evaluate the protein content and antioxidant activity of instant and raw Edible bird’s nest (EBN). This study was conducted using two types of EBNs, which are instant and raw samples. All EBN samples were extracted via three types of extraction method, namely, salt, alkaline, and hot extractions. Lowry’s method was used to analyze protein content and the antioxidant activities were analyzed via free radical scavenging assay 1,1-diphenyl-2-pycrylhydrazyl (DPPH), total phenolic content (TPC) assay and ferric reducing antioxidant power (FRAP). This study showed that the highest protein was 2165.90 µg/mL obtained from raw EBN extracted via alkaline solution. In addition, it was also found that the protein concentration of raw EBN was higher compared to instant EBN for all types of extraction procedures. Results from antioxidant assay showed that there was no significant difference between DPPH and FRAP of both EBN. Moreover, TPC results showed that the there was no phenolic compounds detected via all extraction procedures. AMG Transcend Association 2020 Article PeerReviewed application/pdf en http://eprints.utm.my/93614/1/FaizuanAbdullah2020_EvaluationofProteinContent.pdf Zamri, S. S. and Mahadi, M. and Abdullah, F. and Syafiuddin, A. and Hadibarata, T. (2020) Evaluation of protein content and antioxidant activity of edible bird's nest by various methods. Biointerface Research in Applied Chemistry, 10 (2). pp. 5277-5283. ISSN 2069-5837 http://dx.doi.org/10.1016/j.lwt.2020.109777 DOI: 10.1016/j.lwt.2020.109777
spellingShingle QD Chemistry
Zamri, S. S.
Mahadi, M.
Abdullah, F.
Syafiuddin, A.
Hadibarata, T.
Evaluation of protein content and antioxidant activity of edible bird's nest by various methods
title Evaluation of protein content and antioxidant activity of edible bird's nest by various methods
title_full Evaluation of protein content and antioxidant activity of edible bird's nest by various methods
title_fullStr Evaluation of protein content and antioxidant activity of edible bird's nest by various methods
title_full_unstemmed Evaluation of protein content and antioxidant activity of edible bird's nest by various methods
title_short Evaluation of protein content and antioxidant activity of edible bird's nest by various methods
title_sort evaluation of protein content and antioxidant activity of edible bird s nest by various methods
topic QD Chemistry
url http://eprints.utm.my/93614/1/FaizuanAbdullah2020_EvaluationofProteinContent.pdf
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AT syafiuddina evaluationofproteincontentandantioxidantactivityofediblebirdsnestbyvariousmethods
AT hadibaratat evaluationofproteincontentandantioxidantactivityofediblebirdsnestbyvariousmethods