Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation

Advancement and innovation in bone regeneration, specifically polymeric composite scaffolds, are of high significance for the treatment of bone defects. Xyloglucan (XG) is a polysaccharide biopolymer having a wide variety of regenerative tissue therapeutic applications due to its biocompatibility, i...

Full description

Bibliographic Details
Main Authors: Aslam Khan, M. U., Mehboob, H., Abd. Razak, S. I., Yahya, M. Y., Mohd. Yusof, A. H., Ramlee, M. H., Anand, T. J. S., Hassan, R., Aziz, A., Amin, R.
Format: Article
Published: MDPI AG 2020
Subjects:
_version_ 1796865679458041856
author Aslam Khan, M. U.
Mehboob, H.
Abd. Razak, S. I.
Yahya, M. Y.
Mohd. Yusof, A. H.
Ramlee, M. H.
Anand, T. J. S.
Hassan, R.
Aziz, A.
Amin, R.
author_facet Aslam Khan, M. U.
Mehboob, H.
Abd. Razak, S. I.
Yahya, M. Y.
Mohd. Yusof, A. H.
Ramlee, M. H.
Anand, T. J. S.
Hassan, R.
Aziz, A.
Amin, R.
author_sort Aslam Khan, M. U.
collection ePrints
description Advancement and innovation in bone regeneration, specifically polymeric composite scaffolds, are of high significance for the treatment of bone defects. Xyloglucan (XG) is a polysaccharide biopolymer having a wide variety of regenerative tissue therapeutic applications due to its biocompatibility, in-vitro degradation and cytocompatibility. Current research is focused on the fabrication of polymeric bioactive scaffolds by freeze drying method for nanocomposite materials. The nanocomposite materials have been synthesized from free radical polymerization using n-SiO2 and n-HAp XG and Methacrylic acid (MAAc). Functional group analysis, crystallinity and surface morphology were investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD) and scanning electron microscopy (SEM) techniques, respectively. These bioactive polymeric scaffolds presented interconnected and well-organized porous morphology, controlled precisely by substantial ratios of n-SiO2. The swelling analysis was also performed in different media at varying temperatures (27, 37 and 47 °C) and the mechanical behavior of the dried scaffolds is also investigated. Antibacterial activities of these scaffolds were conducted against pathogenic gram-positive and gram-negative bacteria. Besides, the biological behavior of these scaffolds was evaluated by the Neutral Red dye assay against the MC3T3-E1 cell line. The scaffolds showed interesting properties for bone tissue engineering, including porosity with substantial mechanical strength, biodegradability, biocompatibility and cytocompatibility behavior. The reported polymeric bioactive scaffolds can be aspirant biomaterials for bone tissue engineering to regenerate defecated bone.
first_indexed 2024-03-05T21:00:44Z
format Article
id utm.eprints-93719
institution Universiti Teknologi Malaysia - ePrints
last_indexed 2024-03-05T21:00:44Z
publishDate 2020
publisher MDPI AG
record_format dspace
spelling utm.eprints-937192021-12-31T08:40:22Z http://eprints.utm.my/93719/ Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation Aslam Khan, M. U. Mehboob, H. Abd. Razak, S. I. Yahya, M. Y. Mohd. Yusof, A. H. Ramlee, M. H. Anand, T. J. S. Hassan, R. Aziz, A. Amin, R. Q Science (General) Advancement and innovation in bone regeneration, specifically polymeric composite scaffolds, are of high significance for the treatment of bone defects. Xyloglucan (XG) is a polysaccharide biopolymer having a wide variety of regenerative tissue therapeutic applications due to its biocompatibility, in-vitro degradation and cytocompatibility. Current research is focused on the fabrication of polymeric bioactive scaffolds by freeze drying method for nanocomposite materials. The nanocomposite materials have been synthesized from free radical polymerization using n-SiO2 and n-HAp XG and Methacrylic acid (MAAc). Functional group analysis, crystallinity and surface morphology were investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD) and scanning electron microscopy (SEM) techniques, respectively. These bioactive polymeric scaffolds presented interconnected and well-organized porous morphology, controlled precisely by substantial ratios of n-SiO2. The swelling analysis was also performed in different media at varying temperatures (27, 37 and 47 °C) and the mechanical behavior of the dried scaffolds is also investigated. Antibacterial activities of these scaffolds were conducted against pathogenic gram-positive and gram-negative bacteria. Besides, the biological behavior of these scaffolds was evaluated by the Neutral Red dye assay against the MC3T3-E1 cell line. The scaffolds showed interesting properties for bone tissue engineering, including porosity with substantial mechanical strength, biodegradability, biocompatibility and cytocompatibility behavior. The reported polymeric bioactive scaffolds can be aspirant biomaterials for bone tissue engineering to regenerate defecated bone. MDPI AG 2020 Article PeerReviewed Aslam Khan, M. U. and Mehboob, H. and Abd. Razak, S. I. and Yahya, M. Y. and Mohd. Yusof, A. H. and Ramlee, M. H. and Anand, T. J. S. and Hassan, R. and Aziz, A. and Amin, R. (2020) Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation. Polymers, 12 (6). ISSN 2073-4360 http://dx.doi.org/10.3390/polym12061238 DOI: 10.3390/polym12061238
spellingShingle Q Science (General)
Aslam Khan, M. U.
Mehboob, H.
Abd. Razak, S. I.
Yahya, M. Y.
Mohd. Yusof, A. H.
Ramlee, M. H.
Anand, T. J. S.
Hassan, R.
Aziz, A.
Amin, R.
Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation
title Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation
title_full Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation
title_fullStr Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation
title_full_unstemmed Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation
title_short Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation
title_sort development of polymeric nanocomposite xyloglucan co methacrylic acid hydroxyapatite sio2 scaffold for bone tissue engineering application in vitro antibacterial cytotoxicity and cell culture evaluation
topic Q Science (General)
work_keys_str_mv AT aslamkhanmu developmentofpolymericnanocompositexyloglucancomethacrylicacidhydroxyapatitesio2scaffoldforbonetissueengineeringapplicationinvitroantibacterialcytotoxicityandcellcultureevaluation
AT mehboobh developmentofpolymericnanocompositexyloglucancomethacrylicacidhydroxyapatitesio2scaffoldforbonetissueengineeringapplicationinvitroantibacterialcytotoxicityandcellcultureevaluation
AT abdrazaksi developmentofpolymericnanocompositexyloglucancomethacrylicacidhydroxyapatitesio2scaffoldforbonetissueengineeringapplicationinvitroantibacterialcytotoxicityandcellcultureevaluation
AT yahyamy developmentofpolymericnanocompositexyloglucancomethacrylicacidhydroxyapatitesio2scaffoldforbonetissueengineeringapplicationinvitroantibacterialcytotoxicityandcellcultureevaluation
AT mohdyusofah developmentofpolymericnanocompositexyloglucancomethacrylicacidhydroxyapatitesio2scaffoldforbonetissueengineeringapplicationinvitroantibacterialcytotoxicityandcellcultureevaluation
AT ramleemh developmentofpolymericnanocompositexyloglucancomethacrylicacidhydroxyapatitesio2scaffoldforbonetissueengineeringapplicationinvitroantibacterialcytotoxicityandcellcultureevaluation
AT anandtjs developmentofpolymericnanocompositexyloglucancomethacrylicacidhydroxyapatitesio2scaffoldforbonetissueengineeringapplicationinvitroantibacterialcytotoxicityandcellcultureevaluation
AT hassanr developmentofpolymericnanocompositexyloglucancomethacrylicacidhydroxyapatitesio2scaffoldforbonetissueengineeringapplicationinvitroantibacterialcytotoxicityandcellcultureevaluation
AT aziza developmentofpolymericnanocompositexyloglucancomethacrylicacidhydroxyapatitesio2scaffoldforbonetissueengineeringapplicationinvitroantibacterialcytotoxicityandcellcultureevaluation
AT aminr developmentofpolymericnanocompositexyloglucancomethacrylicacidhydroxyapatitesio2scaffoldforbonetissueengineeringapplicationinvitroantibacterialcytotoxicityandcellcultureevaluation