Optimization of L(+) lactic acid production from solid pineapple waste (SPW) by Rhizopus oryzae NRRL 395
Solid pineapple waste (SPW) is one of the most abundant agricultural wastes found in the tropic region. Improper management could lead to deposition of such waste in the environment causing other problems. Optimized bioconversion into value added products using potent microorganism could be an optio...
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2021
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author | Mohd. Zain, Nor Azimah Aziman, Siti Nurbalqis Suhaimi, Mohd. Suardi Idris, Ani |
author_facet | Mohd. Zain, Nor Azimah Aziman, Siti Nurbalqis Suhaimi, Mohd. Suardi Idris, Ani |
author_sort | Mohd. Zain, Nor Azimah |
collection | ePrints |
description | Solid pineapple waste (SPW) is one of the most abundant agricultural wastes found in the tropic region. Improper management could lead to deposition of such waste in the environment causing other problems. Optimized bioconversion into value added products using potent microorganism could be an option to manage SPW. In this study, Rhizopus oryzae NRRL 395 was used to convert SPW to L(+) lactic acid through solid-state fermentation (SSF). The lactic acid production, reducing sugar utilization and by-product formation were studied using central composite design (CCD) after screening step using 2 level Factorial Design (2LFD). Studied parameters include moisture content (45 to 85% w/w), incubation time (0 to 7 days), temperature (25 to 45 °C), pH (4 to 8), and inoculum size (1 × 105 to 1 × 109 spores/g). During 2LFD, the highest lactic acid concentration obtained was 84.85 mg/g under the condition of 60% w/w of moisture content, 3 days of incubation, 35 °C, pH of 7 and 1 × 107 spores/g. Based on the condition suggested by CCD, the maximum lactic acid concentration of 103.69 mg/g was obtained with the following optimized SSF conditions: 67.53% w/w of moisture content, 3 days of incubation, temperature of 32.2 °C, pH of 5.6, and inoculum size of 1 × 107 spores/g. Graphic Abstract: [Figure not available: see fulltext.]. |
first_indexed | 2024-03-05T21:03:24Z |
format | Article |
id | utm.eprints-94593 |
institution | Universiti Teknologi Malaysia - ePrints |
last_indexed | 2024-03-05T21:03:24Z |
publishDate | 2021 |
publisher | Springer |
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spelling | utm.eprints-945932022-03-31T15:48:16Z http://eprints.utm.my/94593/ Optimization of L(+) lactic acid production from solid pineapple waste (SPW) by Rhizopus oryzae NRRL 395 Mohd. Zain, Nor Azimah Aziman, Siti Nurbalqis Suhaimi, Mohd. Suardi Idris, Ani TP Chemical technology Solid pineapple waste (SPW) is one of the most abundant agricultural wastes found in the tropic region. Improper management could lead to deposition of such waste in the environment causing other problems. Optimized bioconversion into value added products using potent microorganism could be an option to manage SPW. In this study, Rhizopus oryzae NRRL 395 was used to convert SPW to L(+) lactic acid through solid-state fermentation (SSF). The lactic acid production, reducing sugar utilization and by-product formation were studied using central composite design (CCD) after screening step using 2 level Factorial Design (2LFD). Studied parameters include moisture content (45 to 85% w/w), incubation time (0 to 7 days), temperature (25 to 45 °C), pH (4 to 8), and inoculum size (1 × 105 to 1 × 109 spores/g). During 2LFD, the highest lactic acid concentration obtained was 84.85 mg/g under the condition of 60% w/w of moisture content, 3 days of incubation, 35 °C, pH of 7 and 1 × 107 spores/g. Based on the condition suggested by CCD, the maximum lactic acid concentration of 103.69 mg/g was obtained with the following optimized SSF conditions: 67.53% w/w of moisture content, 3 days of incubation, temperature of 32.2 °C, pH of 5.6, and inoculum size of 1 × 107 spores/g. Graphic Abstract: [Figure not available: see fulltext.]. Springer 2021 Article PeerReviewed Mohd. Zain, Nor Azimah and Aziman, Siti Nurbalqis and Suhaimi, Mohd. Suardi and Idris, Ani (2021) Optimization of L(+) lactic acid production from solid pineapple waste (SPW) by Rhizopus oryzae NRRL 395. Journal of Polymers and the Environment, 29 (1). pp. 230-249. ISSN 1566-2543 http://dx.doi.org/10.1007/s10924-020-01862-0 |
spellingShingle | TP Chemical technology Mohd. Zain, Nor Azimah Aziman, Siti Nurbalqis Suhaimi, Mohd. Suardi Idris, Ani Optimization of L(+) lactic acid production from solid pineapple waste (SPW) by Rhizopus oryzae NRRL 395 |
title | Optimization of L(+) lactic acid production from solid pineapple waste (SPW) by Rhizopus oryzae NRRL 395 |
title_full | Optimization of L(+) lactic acid production from solid pineapple waste (SPW) by Rhizopus oryzae NRRL 395 |
title_fullStr | Optimization of L(+) lactic acid production from solid pineapple waste (SPW) by Rhizopus oryzae NRRL 395 |
title_full_unstemmed | Optimization of L(+) lactic acid production from solid pineapple waste (SPW) by Rhizopus oryzae NRRL 395 |
title_short | Optimization of L(+) lactic acid production from solid pineapple waste (SPW) by Rhizopus oryzae NRRL 395 |
title_sort | optimization of l lactic acid production from solid pineapple waste spw by rhizopus oryzae nrrl 395 |
topic | TP Chemical technology |
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