Limitation of nutrients stimulates musty odor production by streptomyces sp. Isolated from a tropical environment
Musty odor production by actinomycetes is usually related to the presence of geosmin and 2-methylisoborneol (2-MIB), which are synthesized by enzymes encoded by the geoA and tpc genes, re-spectively. Streptomyces spp. strain S10, which was isolated from a water reservoir in Malaysia, has the ability...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Microbiology Research Foundation
2021
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| Online Access: | http://eprints.utm.my/94776/1/ShahirahShudirman2021_LimitationofNutrientsStimulatesMusty.pdf |
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| author | Shudirman, S. Kassim, A. A. Anuar, N. S. S. Utsumi, M. Shimizu, K. Yuzir, M. A. M. Noor, M. J. M. N. Akhir, F. N. M. |
| author_facet | Shudirman, S. Kassim, A. A. Anuar, N. S. S. Utsumi, M. Shimizu, K. Yuzir, M. A. M. Noor, M. J. M. N. Akhir, F. N. M. |
| author_sort | Shudirman, S. |
| collection | ePrints |
| description | Musty odor production by actinomycetes is usually related to the presence of geosmin and 2-methylisoborneol (2-MIB), which are synthesized by enzymes encoded by the geoA and tpc genes, re-spectively. Streptomyces spp. strain S10, which was isolated from a water reservoir in Malaysia, has the ability to produce geosmin when cultivated in a basal salt (BS) solid medium, but no 2-MIB production occurred during growth in BS medium. Strain S10 could produce higher levels of geosmin when the phosphate concentration was limited to 0.05 mg/L, with a yield of 17.53 ± 3.12 × 105 ng/L, compared with growth in BS medium. Interestingly, 2-MIB production was suddenly detected when the nitrate concentration was limited to 1.0 mg/L, with a yield of 1.4 ± 0.11 × 105 ng/L. Therefore, it was concluded that phosphate-and nitrate-limiting conditions could induce the initial production of geosmin and 2-MIB by strain S10. Furthermore, a positive amplicon of geoA was detected in strain S10, but no tpc amplicon was detected by PCR analysis. Draft genome sequence analysis showed that one open reading frame (ORF) contained a conserved motif of geosmin synthase with 95% identity with geoA in Streptomyces coelicolor A3 (2). In the case of the tpc genes, it was found that one ORF showed 23% identity to the known tpc gene in S. coelicolor A3(2), but strain S10 lacked one motif in the N-terminus. |
| first_indexed | 2024-03-05T21:03:57Z |
| format | Article |
| id | utm.eprints-94776 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T21:03:57Z |
| publishDate | 2021 |
| publisher | Microbiology Research Foundation |
| record_format | dspace |
| spelling | utm.eprints-947762022-04-29T21:54:12Z http://eprints.utm.my/94776/ Limitation of nutrients stimulates musty odor production by streptomyces sp. Isolated from a tropical environment Shudirman, S. Kassim, A. A. Anuar, N. S. S. Utsumi, M. Shimizu, K. Yuzir, M. A. M. Noor, M. J. M. N. Akhir, F. N. M. T Technology (General) Musty odor production by actinomycetes is usually related to the presence of geosmin and 2-methylisoborneol (2-MIB), which are synthesized by enzymes encoded by the geoA and tpc genes, re-spectively. Streptomyces spp. strain S10, which was isolated from a water reservoir in Malaysia, has the ability to produce geosmin when cultivated in a basal salt (BS) solid medium, but no 2-MIB production occurred during growth in BS medium. Strain S10 could produce higher levels of geosmin when the phosphate concentration was limited to 0.05 mg/L, with a yield of 17.53 ± 3.12 × 105 ng/L, compared with growth in BS medium. Interestingly, 2-MIB production was suddenly detected when the nitrate concentration was limited to 1.0 mg/L, with a yield of 1.4 ± 0.11 × 105 ng/L. Therefore, it was concluded that phosphate-and nitrate-limiting conditions could induce the initial production of geosmin and 2-MIB by strain S10. Furthermore, a positive amplicon of geoA was detected in strain S10, but no tpc amplicon was detected by PCR analysis. Draft genome sequence analysis showed that one open reading frame (ORF) contained a conserved motif of geosmin synthase with 95% identity with geoA in Streptomyces coelicolor A3 (2). In the case of the tpc genes, it was found that one ORF showed 23% identity to the known tpc gene in S. coelicolor A3(2), but strain S10 lacked one motif in the N-terminus. Microbiology Research Foundation 2021 Article PeerReviewed application/pdf en http://eprints.utm.my/94776/1/ShahirahShudirman2021_LimitationofNutrientsStimulatesMusty.pdf Shudirman, S. and Kassim, A. A. and Anuar, N. S. S. and Utsumi, M. and Shimizu, K. and Yuzir, M. A. M. and Noor, M. J. M. N. and Akhir, F. N. M. (2021) Limitation of nutrients stimulates musty odor production by streptomyces sp. Isolated from a tropical environment. Journal of General and Applied Microbiology, 67 (3). ISSN 0022-1260 http://dx.doi.org/10.2323/jgam.2020.08.001 DOI: 10.2323/jgam.2020.08.001 |
| spellingShingle | T Technology (General) Shudirman, S. Kassim, A. A. Anuar, N. S. S. Utsumi, M. Shimizu, K. Yuzir, M. A. M. Noor, M. J. M. N. Akhir, F. N. M. Limitation of nutrients stimulates musty odor production by streptomyces sp. Isolated from a tropical environment |
| title | Limitation of nutrients stimulates musty odor production by streptomyces sp. Isolated from a tropical environment |
| title_full | Limitation of nutrients stimulates musty odor production by streptomyces sp. Isolated from a tropical environment |
| title_fullStr | Limitation of nutrients stimulates musty odor production by streptomyces sp. Isolated from a tropical environment |
| title_full_unstemmed | Limitation of nutrients stimulates musty odor production by streptomyces sp. Isolated from a tropical environment |
| title_short | Limitation of nutrients stimulates musty odor production by streptomyces sp. Isolated from a tropical environment |
| title_sort | limitation of nutrients stimulates musty odor production by streptomyces sp isolated from a tropical environment |
| topic | T Technology (General) |
| url | http://eprints.utm.my/94776/1/ShahirahShudirman2021_LimitationofNutrientsStimulatesMusty.pdf |
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