Integrated approach for species identification and quality analysis for Labisia pumila using DNA barcoding and HPLC
Labisia pumila is a precious herb in Southeast Asia that is traditionally used as a health supplement and has been extensively commercialized due to its claimed therapeutic properties in boosting a healthy female reproductive system. Indigenous people used these plants by boiling the leaves; however...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021
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| Online Access: | http://eprints.utm.my/95545/1/AlinaWagiran2021_IntegratedApproachforSpeciesIdentificationandQualityAnalysis.pdf |
| _version_ | 1796866041611026432 |
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| author | Ahmad Tarmizi, Auni Aqilah Wagiran, Alina Mohd. Salleh, Faezah Suan Chua, Lee Abdullah, Farah Izana Hasham, Rosnani Mostafiz, Suraiya |
| author_facet | Ahmad Tarmizi, Auni Aqilah Wagiran, Alina Mohd. Salleh, Faezah Suan Chua, Lee Abdullah, Farah Izana Hasham, Rosnani Mostafiz, Suraiya |
| author_sort | Ahmad Tarmizi, Auni Aqilah |
| collection | ePrints |
| description | Labisia pumila is a precious herb in Southeast Asia that is traditionally used as a health supplement and has been extensively commercialized due to its claimed therapeutic properties in boosting a healthy female reproductive system. Indigenous people used these plants by boiling the leaves; however, in recent years it has been marketed as powdered or capsuled products. Accordingly, accuracy in determination of the authenticity of these modern herbal products has faced great challenges. Lack of authenticity is a public health risk because incorrectly used herbal species can cause adverse effects. Hence, any measures that may aid product authentication would be beneficial. Given the widespread use of Labisia herbal products, the current study focuses on authenticity testing via an integral approach of DNA barcoding and qualitative analysis using HPLC. This study successfully generated DNA reference barcodes (ITS2 and rbcL) for L.pumila var. alata and pumila. The DNA barcode that was generated was then used to identify species of Labisia pumila in herbal medicinal products, while HPLC was utilized to determine their quality. The findings through the synergistic approach (DNA barcode and HPLC) implemented in this study indicate the importance of both methods in providing the strong evidence required for the identification of true species and to examine the authenticity of such herbal medicinal products. |
| first_indexed | 2024-03-05T21:06:16Z |
| format | Article |
| id | utm.eprints-95545 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T21:06:16Z |
| publishDate | 2021 |
| publisher | MDPI AG |
| record_format | dspace |
| spelling | utm.eprints-955452022-07-31T06:12:16Z http://eprints.utm.my/95545/ Integrated approach for species identification and quality analysis for Labisia pumila using DNA barcoding and HPLC Ahmad Tarmizi, Auni Aqilah Wagiran, Alina Mohd. Salleh, Faezah Suan Chua, Lee Abdullah, Farah Izana Hasham, Rosnani Mostafiz, Suraiya QD Chemistry Labisia pumila is a precious herb in Southeast Asia that is traditionally used as a health supplement and has been extensively commercialized due to its claimed therapeutic properties in boosting a healthy female reproductive system. Indigenous people used these plants by boiling the leaves; however, in recent years it has been marketed as powdered or capsuled products. Accordingly, accuracy in determination of the authenticity of these modern herbal products has faced great challenges. Lack of authenticity is a public health risk because incorrectly used herbal species can cause adverse effects. Hence, any measures that may aid product authentication would be beneficial. Given the widespread use of Labisia herbal products, the current study focuses on authenticity testing via an integral approach of DNA barcoding and qualitative analysis using HPLC. This study successfully generated DNA reference barcodes (ITS2 and rbcL) for L.pumila var. alata and pumila. The DNA barcode that was generated was then used to identify species of Labisia pumila in herbal medicinal products, while HPLC was utilized to determine their quality. The findings through the synergistic approach (DNA barcode and HPLC) implemented in this study indicate the importance of both methods in providing the strong evidence required for the identification of true species and to examine the authenticity of such herbal medicinal products. MDPI AG 2021 Article PeerReviewed application/pdf en http://eprints.utm.my/95545/1/AlinaWagiran2021_IntegratedApproachforSpeciesIdentificationandQualityAnalysis.pdf Ahmad Tarmizi, Auni Aqilah and Wagiran, Alina and Mohd. Salleh, Faezah and Suan Chua, Lee and Abdullah, Farah Izana and Hasham, Rosnani and Mostafiz, Suraiya (2021) Integrated approach for species identification and quality analysis for Labisia pumila using DNA barcoding and HPLC. Plants, 10 (4). p. 717. ISSN 2223-7747 http://dx.doi.org/10.3390/plants10040717 DOI:10.3390/plants10040717 |
| spellingShingle | QD Chemistry Ahmad Tarmizi, Auni Aqilah Wagiran, Alina Mohd. Salleh, Faezah Suan Chua, Lee Abdullah, Farah Izana Hasham, Rosnani Mostafiz, Suraiya Integrated approach for species identification and quality analysis for Labisia pumila using DNA barcoding and HPLC |
| title | Integrated approach for species identification and quality analysis for Labisia pumila using DNA barcoding and HPLC |
| title_full | Integrated approach for species identification and quality analysis for Labisia pumila using DNA barcoding and HPLC |
| title_fullStr | Integrated approach for species identification and quality analysis for Labisia pumila using DNA barcoding and HPLC |
| title_full_unstemmed | Integrated approach for species identification and quality analysis for Labisia pumila using DNA barcoding and HPLC |
| title_short | Integrated approach for species identification and quality analysis for Labisia pumila using DNA barcoding and HPLC |
| title_sort | integrated approach for species identification and quality analysis for labisia pumila using dna barcoding and hplc |
| topic | QD Chemistry |
| url | http://eprints.utm.my/95545/1/AlinaWagiran2021_IntegratedApproachforSpeciesIdentificationandQualityAnalysis.pdf |
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