Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite scaffolds for fractured bone healing
The importance of bone scaffolds has increased many folds in the last few years; however, during bone implantation, bacterial infections compromise the implantation and tissue regeneration. This work is focused on this issue while not compromising on the properties of a scaffold for bone regeneratio...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
John Wiley and Sons Ltd
2021
|
| Subjects: |
| _version_ | 1796866094718255104 |
|---|---|
| author | Aslam Khan, Muhammad Umar Haider, Adnan Abd. Razak, Saiful Izwan Abdul Kadir, Mohammed Rafiq Haider, Sajjad A. Shah, Saqlain Hasan, Anwarul Khan, Rawaiz Khan, Salah-Ud Din Shakir, Imran |
| author_facet | Aslam Khan, Muhammad Umar Haider, Adnan Abd. Razak, Saiful Izwan Abdul Kadir, Mohammed Rafiq Haider, Sajjad A. Shah, Saqlain Hasan, Anwarul Khan, Rawaiz Khan, Salah-Ud Din Shakir, Imran |
| author_sort | Aslam Khan, Muhammad Umar |
| collection | ePrints |
| description | The importance of bone scaffolds has increased many folds in the last few years; however, during bone implantation, bacterial infections compromise the implantation and tissue regeneration. This work is focused on this issue while not compromising on the properties of a scaffold for bone regeneration. Biocomposite scaffolds (BS) were fabricated via the freeze–drying technique. The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer–Emmett–Teller, universal testing machine Instron) techniques. Antibacterial, cellular, and hemocompatibility assays were performed using standard protocols. FT-IR confirmed the interactions of all the components. SEM illustrated porous and interconnected porous morphology. The percentage porosity was in the range of 49.75%–67.28%, and the pore size was 215.65–470.87 µm. The pore size was perfect for cellular penetration. Thus, cells showed significant proliferation onto these scaffolds. X-ray studies confirmed the presence of nanohydroxyapatite and graphene oxide (GO). The cell viability was 85%–98% (BS1–BS3), which shows no significant toxicity of the biocomposite. Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis. The ultimate compression strength for the biocomposites increased from 4.05 to 7.94 with an increase in the GO content. These exciting results revealed that this material has the potential for possible application in bone tissue engineering. |
| first_indexed | 2024-03-05T21:07:02Z |
| format | Article |
| id | utm.eprints-95801 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T21:07:02Z |
| publishDate | 2021 |
| publisher | John Wiley and Sons Ltd |
| record_format | dspace |
| spelling | utm.eprints-958012022-05-31T13:19:24Z http://eprints.utm.my/95801/ Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite scaffolds for fractured bone healing Aslam Khan, Muhammad Umar Haider, Adnan Abd. Razak, Saiful Izwan Abdul Kadir, Mohammed Rafiq Haider, Sajjad A. Shah, Saqlain Hasan, Anwarul Khan, Rawaiz Khan, Salah-Ud Din Shakir, Imran QD Chemistry The importance of bone scaffolds has increased many folds in the last few years; however, during bone implantation, bacterial infections compromise the implantation and tissue regeneration. This work is focused on this issue while not compromising on the properties of a scaffold for bone regeneration. Biocomposite scaffolds (BS) were fabricated via the freeze–drying technique. The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer–Emmett–Teller, universal testing machine Instron) techniques. Antibacterial, cellular, and hemocompatibility assays were performed using standard protocols. FT-IR confirmed the interactions of all the components. SEM illustrated porous and interconnected porous morphology. The percentage porosity was in the range of 49.75%–67.28%, and the pore size was 215.65–470.87 µm. The pore size was perfect for cellular penetration. Thus, cells showed significant proliferation onto these scaffolds. X-ray studies confirmed the presence of nanohydroxyapatite and graphene oxide (GO). The cell viability was 85%–98% (BS1–BS3), which shows no significant toxicity of the biocomposite. Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis. The ultimate compression strength for the biocomposites increased from 4.05 to 7.94 with an increase in the GO content. These exciting results revealed that this material has the potential for possible application in bone tissue engineering. John Wiley and Sons Ltd 2021 Article PeerReviewed Aslam Khan, Muhammad Umar and Haider, Adnan and Abd. Razak, Saiful Izwan and Abdul Kadir, Mohammed Rafiq and Haider, Sajjad and A. Shah, Saqlain and Hasan, Anwarul and Khan, Rawaiz and Khan, Salah-Ud Din and Shakir, Imran (2021) Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite scaffolds for fractured bone healing. Journal of Tissue Engineering and Regenerative Medicine, 15 (4). pp. 322-335. ISSN 1932-6254 http://dx.doi.org/10.1002/term.3168 |
| spellingShingle | QD Chemistry Aslam Khan, Muhammad Umar Haider, Adnan Abd. Razak, Saiful Izwan Abdul Kadir, Mohammed Rafiq Haider, Sajjad A. Shah, Saqlain Hasan, Anwarul Khan, Rawaiz Khan, Salah-Ud Din Shakir, Imran Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite scaffolds for fractured bone healing |
| title | Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite scaffolds for fractured bone healing |
| title_full | Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite scaffolds for fractured bone healing |
| title_fullStr | Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite scaffolds for fractured bone healing |
| title_full_unstemmed | Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite scaffolds for fractured bone healing |
| title_short | Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite scaffolds for fractured bone healing |
| title_sort | arabinoxylan graphene oxide nhap nps pva bionano composite scaffolds for fractured bone healing |
| topic | QD Chemistry |
| work_keys_str_mv | AT aslamkhanmuhammadumar arabinoxylangrapheneoxidenhapnpspvabionanocompositescaffoldsforfracturedbonehealing AT haideradnan arabinoxylangrapheneoxidenhapnpspvabionanocompositescaffoldsforfracturedbonehealing AT abdrazaksaifulizwan arabinoxylangrapheneoxidenhapnpspvabionanocompositescaffoldsforfracturedbonehealing AT abdulkadirmohammedrafiq arabinoxylangrapheneoxidenhapnpspvabionanocompositescaffoldsforfracturedbonehealing AT haidersajjad arabinoxylangrapheneoxidenhapnpspvabionanocompositescaffoldsforfracturedbonehealing AT ashahsaqlain arabinoxylangrapheneoxidenhapnpspvabionanocompositescaffoldsforfracturedbonehealing AT hasananwarul arabinoxylangrapheneoxidenhapnpspvabionanocompositescaffoldsforfracturedbonehealing AT khanrawaiz arabinoxylangrapheneoxidenhapnpspvabionanocompositescaffoldsforfracturedbonehealing AT khansalahuddin arabinoxylangrapheneoxidenhapnpspvabionanocompositescaffoldsforfracturedbonehealing AT shakirimran arabinoxylangrapheneoxidenhapnpspvabionanocompositescaffoldsforfracturedbonehealing |