Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives
This study focused on kinetics of levan yield by Bacillus subtilis M, in a 150 L stirred tank bioreactor under controlled pH conditions. The optimized production medium was composed of (g/L): commercial sucrose 100.0, yeast extract 2.0, K2HPO4 3.0 and MgSO4·7H2O 0.2; an increase in both carbohydrate...
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| Format: | Article |
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Elsevier B.V.
2019
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| _version_ | 1796866324803092480 |
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| author | Ragab, Tamer I. M. Abd. Malek, Roslinda Elsehemy, Islam A. Farag, Mohamed M. S. Salama, Bassem M. Abd. EL-Baseer, Mohamed A. Gamal-Eldeen, Amira M. El Enshasy, Hesham A. Esawy, Mona A. |
| author_facet | Ragab, Tamer I. M. Abd. Malek, Roslinda Elsehemy, Islam A. Farag, Mohamed M. S. Salama, Bassem M. Abd. EL-Baseer, Mohamed A. Gamal-Eldeen, Amira M. El Enshasy, Hesham A. Esawy, Mona A. |
| author_sort | Ragab, Tamer I. M. |
| collection | ePrints |
| description | This study focused on kinetics of levan yield by Bacillus subtilis M, in a 150 L stirred tank bioreactor under controlled pH conditions. The optimized production medium was composed of (g/L): commercial sucrose 100.0, yeast extract 2.0, K2HPO4 3.0 and MgSO4·7H2O 0.2; an increase in both carbohydrates consumption and cell growth depended on increasing the size of the stirred tank bioreactor from 16 L to 150 L. The highest levansucrase production (63.4 U/mL) and levan yield of 47 g/L was obtained after 24 h. Also, the specific levan yield (Yp/x) which reflects the cell productivity increased with the size increase of the stirred tank bioreactor and reached its maximum value of about 29.4 g/g cells. These results suggested that B. subtilis M could play an important role in levan yield on a large scale in the future. Chemical modifications of B. subtilis M crude levan (CL) into sulfated (SL), phosphorylated (PL), and carboxymethylated levans (CML) were done. The difference in CL structure and its derivatives was detected by FT-IR transmission spectrum. The cytotoxicity of CL and its derivatives were evaluated by HepGII, Mcf-7 and CaCo-2. In general most tested levans forms had no significant cytotoxicity effect. In fact, the carboxymethylated and phosphrylated forms had a lower anti-cancer effect than CL. On the other hand, SL had the highest cytotoxicity showing SL had a significant anti-cancer effect. The results of cytotoxicity and cell viability were statistically analyzed using three-way ANOVA. |
| first_indexed | 2024-03-05T21:10:27Z |
| format | Article |
| id | utm.eprints-96944 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T21:10:27Z |
| publishDate | 2019 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | utm.eprints-969442022-09-04T07:26:27Z http://eprints.utm.my/96944/ Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives Ragab, Tamer I. M. Abd. Malek, Roslinda Elsehemy, Islam A. Farag, Mohamed M. S. Salama, Bassem M. Abd. EL-Baseer, Mohamed A. Gamal-Eldeen, Amira M. El Enshasy, Hesham A. Esawy, Mona A. QD Chemistry This study focused on kinetics of levan yield by Bacillus subtilis M, in a 150 L stirred tank bioreactor under controlled pH conditions. The optimized production medium was composed of (g/L): commercial sucrose 100.0, yeast extract 2.0, K2HPO4 3.0 and MgSO4·7H2O 0.2; an increase in both carbohydrates consumption and cell growth depended on increasing the size of the stirred tank bioreactor from 16 L to 150 L. The highest levansucrase production (63.4 U/mL) and levan yield of 47 g/L was obtained after 24 h. Also, the specific levan yield (Yp/x) which reflects the cell productivity increased with the size increase of the stirred tank bioreactor and reached its maximum value of about 29.4 g/g cells. These results suggested that B. subtilis M could play an important role in levan yield on a large scale in the future. Chemical modifications of B. subtilis M crude levan (CL) into sulfated (SL), phosphorylated (PL), and carboxymethylated levans (CML) were done. The difference in CL structure and its derivatives was detected by FT-IR transmission spectrum. The cytotoxicity of CL and its derivatives were evaluated by HepGII, Mcf-7 and CaCo-2. In general most tested levans forms had no significant cytotoxicity effect. In fact, the carboxymethylated and phosphrylated forms had a lower anti-cancer effect than CL. On the other hand, SL had the highest cytotoxicity showing SL had a significant anti-cancer effect. The results of cytotoxicity and cell viability were statistically analyzed using three-way ANOVA. Elsevier B.V. 2019 Article PeerReviewed Ragab, Tamer I. M. and Abd. Malek, Roslinda and Elsehemy, Islam A. and Farag, Mohamed M. S. and Salama, Bassem M. and Abd. EL-Baseer, Mohamed A. and Gamal-Eldeen, Amira M. and El Enshasy, Hesham A. and Esawy, Mona A. (2019) Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives. Journal of Bioscience and Bioengineering, 127 (6). pp. 655-662. ISSN 1389-1723 http://dx.doi.org/10.1016/j.jbiosc.2018.09.008 DOI : 10.1016/j.jbiosc.2018.09.008 |
| spellingShingle | QD Chemistry Ragab, Tamer I. M. Abd. Malek, Roslinda Elsehemy, Islam A. Farag, Mohamed M. S. Salama, Bassem M. Abd. EL-Baseer, Mohamed A. Gamal-Eldeen, Amira M. El Enshasy, Hesham A. Esawy, Mona A. Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives |
| title | Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives |
| title_full | Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives |
| title_fullStr | Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives |
| title_full_unstemmed | Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives |
| title_short | Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives |
| title_sort | scaling up of levan yield in bacillus subtilis m and cytotoxicity study on levan and its derivatives |
| topic | QD Chemistry |
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