Normal soaking extraction (NSE) of rotenone from Derris elliptica
Derris elliptica or the tuba plant contains rotenone, bio-active compound known that has the potential to be used as bio-pesticide. Bio-pesticide is the best-known alternative bio-pesticide which has the potential to replace the use of conventional pesticides, as it is more environmentally friendly....
| Main Authors: | , , , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2004
|
| Subjects: | |
| Online Access: | http://eprints.utm.my/9798/1/ZubairiSI2004_normal_soaking_extraction_%28NSE%29_of_rotenone.pdf |
| _version_ | 1825910380668387328 |
|---|---|
| author | Zubairi, Saiful Irwan Sarmidi, M. R. Aziz, R. A. Ramli, M. K. A. Latip, R. Nordin, N. I. A. |
| author_facet | Zubairi, Saiful Irwan Sarmidi, M. R. Aziz, R. A. Ramli, M. K. A. Latip, R. Nordin, N. I. A. |
| author_sort | Zubairi, Saiful Irwan |
| collection | ePrints |
| description | Derris elliptica or the tuba plant contains rotenone, bio-active compound known that has the potential to be used as bio-pesticide. Bio-pesticide is the best-known alternative bio-pesticide which has the potential to replace the use of conventional pesticides, as it is more environmentally friendly. The main objective of the paper is to obtain the best extraction solvent for optimizing the yield in rotenone extraction. Rotenone was extracted from Derris root using three different parts of roots and three types of solvent in Normal Soaking Extraction (NSE). The types of Derris roots are: (1) Fine root (2) Coarse root (3) Stem. The three types of solvent system are: (1) Acetone 95 % (v/v), (2) Chloroform 99.9 % (v/v) and (3) a mixture of ethanol: H2O (9:1) added with oxalic acid (1mg/ml). The liquid crude extracts were further cleaned up to remove the fine debris of roots. The presence of rotenone was confirmed using qualitative analysis Thin Layer Chromatography (TLC) and thereupon the determination of rotenone content was carried out using High Performance Liquid Chromatography (HPLC). From the results obtained, it was found that the Normal Soaking Extraction (NSE) using acetone 95 % (v/v) was the best method to extract the highest yield of rotenone; 1.14 % (w/w). |
| first_indexed | 2024-03-05T18:16:12Z |
| format | Conference or Workshop Item |
| id | utm.eprints-9798 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T18:16:12Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | utm.eprints-97982010-06-02T02:03:31Z http://eprints.utm.my/9798/ Normal soaking extraction (NSE) of rotenone from Derris elliptica Zubairi, Saiful Irwan Sarmidi, M. R. Aziz, R. A. Ramli, M. K. A. Latip, R. Nordin, N. I. A. TP Chemical technology QH301 Biology Derris elliptica or the tuba plant contains rotenone, bio-active compound known that has the potential to be used as bio-pesticide. Bio-pesticide is the best-known alternative bio-pesticide which has the potential to replace the use of conventional pesticides, as it is more environmentally friendly. The main objective of the paper is to obtain the best extraction solvent for optimizing the yield in rotenone extraction. Rotenone was extracted from Derris root using three different parts of roots and three types of solvent in Normal Soaking Extraction (NSE). The types of Derris roots are: (1) Fine root (2) Coarse root (3) Stem. The three types of solvent system are: (1) Acetone 95 % (v/v), (2) Chloroform 99.9 % (v/v) and (3) a mixture of ethanol: H2O (9:1) added with oxalic acid (1mg/ml). The liquid crude extracts were further cleaned up to remove the fine debris of roots. The presence of rotenone was confirmed using qualitative analysis Thin Layer Chromatography (TLC) and thereupon the determination of rotenone content was carried out using High Performance Liquid Chromatography (HPLC). From the results obtained, it was found that the Normal Soaking Extraction (NSE) using acetone 95 % (v/v) was the best method to extract the highest yield of rotenone; 1.14 % (w/w). 2004-08 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/9798/1/ZubairiSI2004_normal_soaking_extraction_%28NSE%29_of_rotenone.pdf Zubairi, Saiful Irwan and Sarmidi, M. R. and Aziz, R. A. and Ramli, M. K. A. and Latip, R. and Nordin, N. I. A. (2004) Normal soaking extraction (NSE) of rotenone from Derris elliptica. In: Proceeding of Simposium Kimia Analisis Kebangsaan (SKAM-17), 26-28 August 2004, Swiss Garden Resort, Pahang. |
| spellingShingle | TP Chemical technology QH301 Biology Zubairi, Saiful Irwan Sarmidi, M. R. Aziz, R. A. Ramli, M. K. A. Latip, R. Nordin, N. I. A. Normal soaking extraction (NSE) of rotenone from Derris elliptica |
| title | Normal soaking extraction (NSE) of rotenone from Derris elliptica |
| title_full | Normal soaking extraction (NSE) of rotenone from Derris elliptica |
| title_fullStr | Normal soaking extraction (NSE) of rotenone from Derris elliptica |
| title_full_unstemmed | Normal soaking extraction (NSE) of rotenone from Derris elliptica |
| title_short | Normal soaking extraction (NSE) of rotenone from Derris elliptica |
| title_sort | normal soaking extraction nse of rotenone from derris elliptica |
| topic | TP Chemical technology QH301 Biology |
| url | http://eprints.utm.my/9798/1/ZubairiSI2004_normal_soaking_extraction_%28NSE%29_of_rotenone.pdf |
| work_keys_str_mv | AT zubairisaifulirwan normalsoakingextractionnseofrotenonefromderriselliptica AT sarmidimr normalsoakingextractionnseofrotenonefromderriselliptica AT azizra normalsoakingextractionnseofrotenonefromderriselliptica AT ramlimka normalsoakingextractionnseofrotenonefromderriselliptica AT latipr normalsoakingextractionnseofrotenonefromderriselliptica AT nordinnia normalsoakingextractionnseofrotenonefromderriselliptica |