The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito
Rotenone is a bio-active compound extracted from Derris elliptica (Tuba). It has long been used as biopesticide, which is more environmental friendly. Recently, new processing methods have been published on the extraction and usage of rotenone. The objective of this paper is to obtain the effectiven...
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Format: | Conference or Workshop Item |
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2004
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Online Access: | http://eprints.utm.my/9800/1/ZubairiSI2004_the_effect_of_rotenone_crude_extract.pdf |
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author | Zubairi, Saiful Irwan Sarmidi, M. R. Aziz, R. A. Latip, R. Said, J. |
author_facet | Zubairi, Saiful Irwan Sarmidi, M. R. Aziz, R. A. Latip, R. Said, J. |
author_sort | Zubairi, Saiful Irwan |
collection | ePrints |
description | Rotenone is a bio-active compound extracted from Derris elliptica (Tuba). It has long been used as biopesticide, which is more environmental friendly. Recently, new processing methods have been published on the extraction and usage of rotenone. The objective of this paper is to obtain the effectiveness of Derris plant crude extract against mosquito larvae. Rotenone crude extract was extracted using Normal Soaking Extraction (NSE) method. Two different solvent ratios were used to extract rotenone which is: (A) Methyl chloride: methanol (1:1) and (B) Methyl chloride: methanol (1:9). One part of both crude extracts using solvent ratios A and B were concentrated further using the rotary evaporator at 40 0C and under 800 mbar vacuum pressures. All the samples (normal and concentrated) were subjected to larvicidal activity testing using mosquito larvae. The samples were diluted to 5 different concentrations (0.01 mg/ml, 0.02 mg/ml, 0.03 mg/ml, 0.04 mg/ml and 0.05 mg/ml) for the biological activity treatment (LC50). Six larvae of Aedes aegypti were used to test each samples. The study found that concentrated crude extract (B) using solvent ratio (1:1) gave optimum mortality of mosquito larvae of 83.33 % at 0.05 mg/ml after 5 hours post treatment. |
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format | Conference or Workshop Item |
id | utm.eprints-9800 |
institution | Universiti Teknologi Malaysia - ePrints |
language | English |
last_indexed | 2024-03-05T18:16:13Z |
publishDate | 2004 |
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spelling | utm.eprints-98002010-06-02T02:03:33Z http://eprints.utm.my/9800/ The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito Zubairi, Saiful Irwan Sarmidi, M. R. Aziz, R. A. Latip, R. Said, J. TP Chemical technology QH301 Biology Rotenone is a bio-active compound extracted from Derris elliptica (Tuba). It has long been used as biopesticide, which is more environmental friendly. Recently, new processing methods have been published on the extraction and usage of rotenone. The objective of this paper is to obtain the effectiveness of Derris plant crude extract against mosquito larvae. Rotenone crude extract was extracted using Normal Soaking Extraction (NSE) method. Two different solvent ratios were used to extract rotenone which is: (A) Methyl chloride: methanol (1:1) and (B) Methyl chloride: methanol (1:9). One part of both crude extracts using solvent ratios A and B were concentrated further using the rotary evaporator at 40 0C and under 800 mbar vacuum pressures. All the samples (normal and concentrated) were subjected to larvicidal activity testing using mosquito larvae. The samples were diluted to 5 different concentrations (0.01 mg/ml, 0.02 mg/ml, 0.03 mg/ml, 0.04 mg/ml and 0.05 mg/ml) for the biological activity treatment (LC50). Six larvae of Aedes aegypti were used to test each samples. The study found that concentrated crude extract (B) using solvent ratio (1:1) gave optimum mortality of mosquito larvae of 83.33 % at 0.05 mg/ml after 5 hours post treatment. 2004-05 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/9800/1/ZubairiSI2004_the_effect_of_rotenone_crude_extract.pdf Zubairi, Saiful Irwan and Sarmidi, M. R. and Aziz, R. A. and Latip, R. and Said, J. (2004) The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito. In: Proceeding of Symposium Biologi Kebangsaan ke-7, 18-20 May 2004, Awana Genting Highlands, Pahang. |
spellingShingle | TP Chemical technology QH301 Biology Zubairi, Saiful Irwan Sarmidi, M. R. Aziz, R. A. Latip, R. Said, J. The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito |
title | The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito |
title_full | The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito |
title_fullStr | The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito |
title_full_unstemmed | The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito |
title_short | The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito |
title_sort | effect of rotenone crude extract from derris elliptica on the larvicidal activity mortality of mosquito |
topic | TP Chemical technology QH301 Biology |
url | http://eprints.utm.my/9800/1/ZubairiSI2004_the_effect_of_rotenone_crude_extract.pdf |
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