Characterisation of a bacterium from Tebrau Strait and screening of microbial genomes for dehalogenases
Current study was to investigate the presence of dehalogenase in the isolated bacterium from Selat Tebrau that can grow on 2,2-dichloropropionic acid (2,2-DCP is an active compound in herbicide Dalapon®). Strain RN1, a Gram-negative and rod in shape was tentatively identified as Enterobacter cancero...
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Brawijaya University
2022
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| author | Oyewusi, Habeebat Adekilekun Raja Mohamed, Raja Nurulhafiza Huyop, Fahrul |
| author_facet | Oyewusi, Habeebat Adekilekun Raja Mohamed, Raja Nurulhafiza Huyop, Fahrul |
| author_sort | Oyewusi, Habeebat Adekilekun |
| collection | ePrints |
| description | Current study was to investigate the presence of dehalogenase in the isolated bacterium from Selat Tebrau that can grow on 2,2-dichloropropionic acid (2,2-DCP is an active compound in herbicide Dalapon®). Strain RN1, a Gram-negative and rod in shape was tentatively identified as Enterobacter cancerogenus based on basic biochemical and the 16S rRNA gene analyses. The calculated cells doubling time were 5.29 hours based on growth of the bacterium in liquid minimal media with 10, 20 and 30 mM of 2,2-DCP, respectively. However, no growth was observed at 40 mM 2,2-DCP liquid minimal medium due to increase in 2,2-DCP toxicity. It was hypoth-esized that, strain RN1 produced dehalogenase(s) which merits a further study of the genomic data of the same genus and species available in the database. A putative dehalogenase, designated as DehRN was located in the published data of Enterobac-ter cancerogenus. Pairwise of DehRN amino acids with known dehalogenase re-sulted in sequence identity of <20%, suggesting a new class of dehalogenase enzyme in the Enterobacter cancerogenus. |
| first_indexed | 2024-03-05T21:15:53Z |
| format | Article |
| id | utm.eprints-98771 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T21:15:53Z |
| publishDate | 2022 |
| publisher | Brawijaya University |
| record_format | dspace |
| spelling | utm.eprints-987712023-02-02T08:36:42Z http://eprints.utm.my/98771/ Characterisation of a bacterium from Tebrau Strait and screening of microbial genomes for dehalogenases Oyewusi, Habeebat Adekilekun Raja Mohamed, Raja Nurulhafiza Huyop, Fahrul Q Science (General) Current study was to investigate the presence of dehalogenase in the isolated bacterium from Selat Tebrau that can grow on 2,2-dichloropropionic acid (2,2-DCP is an active compound in herbicide Dalapon®). Strain RN1, a Gram-negative and rod in shape was tentatively identified as Enterobacter cancerogenus based on basic biochemical and the 16S rRNA gene analyses. The calculated cells doubling time were 5.29 hours based on growth of the bacterium in liquid minimal media with 10, 20 and 30 mM of 2,2-DCP, respectively. However, no growth was observed at 40 mM 2,2-DCP liquid minimal medium due to increase in 2,2-DCP toxicity. It was hypoth-esized that, strain RN1 produced dehalogenase(s) which merits a further study of the genomic data of the same genus and species available in the database. A putative dehalogenase, designated as DehRN was located in the published data of Enterobac-ter cancerogenus. Pairwise of DehRN amino acids with known dehalogenase re-sulted in sequence identity of <20%, suggesting a new class of dehalogenase enzyme in the Enterobacter cancerogenus. Brawijaya University 2022 Article PeerReviewed Oyewusi, Habeebat Adekilekun and Raja Mohamed, Raja Nurulhafiza and Huyop, Fahrul (2022) Characterisation of a bacterium from Tebrau Strait and screening of microbial genomes for dehalogenases. Journal of Tropical Life Science, 12 (2). pp. 199-206. ISSN 2087-5517 http://dx.doi.org/10.11594/jtls.12.02.06 DOI: 10.11594/jtls.12.02.06 |
| spellingShingle | Q Science (General) Oyewusi, Habeebat Adekilekun Raja Mohamed, Raja Nurulhafiza Huyop, Fahrul Characterisation of a bacterium from Tebrau Strait and screening of microbial genomes for dehalogenases |
| title | Characterisation of a bacterium from Tebrau Strait and screening of microbial genomes for dehalogenases |
| title_full | Characterisation of a bacterium from Tebrau Strait and screening of microbial genomes for dehalogenases |
| title_fullStr | Characterisation of a bacterium from Tebrau Strait and screening of microbial genomes for dehalogenases |
| title_full_unstemmed | Characterisation of a bacterium from Tebrau Strait and screening of microbial genomes for dehalogenases |
| title_short | Characterisation of a bacterium from Tebrau Strait and screening of microbial genomes for dehalogenases |
| title_sort | characterisation of a bacterium from tebrau strait and screening of microbial genomes for dehalogenases |
| topic | Q Science (General) |
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