The prevention impact of the green algal extract against genetic toxicity and antioxidant enzyme alteration in the Mozambique tilapia
Algal studies are primary for ecological risk assessment and toxicology by evaluating lethal and sub-lethal toxic impacts of potential toxicants on inhabitants of numerous ecosystems. Dunaliella salina, a green marine alga, is characterized by its carotenoid accumulation and is widely used in many h...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Egyptian Society for the Development of Fisheries and Human Health
2022
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| Online Access: | http://eprints.utm.my/99610/1/HeshamElEnshasy2022_ThePreventionImpactoftheGreenAlgalExtract.pdf |
| _version_ | 1796866789703942144 |
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| author | Khalil, Wagdy K. B. Bassem, Samah M. Sabry, Noha M. Abd. ElTawab, Marwa I. El Enshasy, Hesham Temraz, Tarek A. Guerriero, Giulia Abdel-Gawad, Fagr Kh. |
| author_facet | Khalil, Wagdy K. B. Bassem, Samah M. Sabry, Noha M. Abd. ElTawab, Marwa I. El Enshasy, Hesham Temraz, Tarek A. Guerriero, Giulia Abdel-Gawad, Fagr Kh. |
| author_sort | Khalil, Wagdy K. B. |
| collection | ePrints |
| description | Algal studies are primary for ecological risk assessment and toxicology by evaluating lethal and sub-lethal toxic impacts of potential toxicants on inhabitants of numerous ecosystems. Dunaliella salina, a green marine alga, is characterized by its carotenoid accumulation and is widely used in many health and nutritional products. Our experiment was designed to evaluate algal extract's ability to inhibit genetic alterations induced by mutagen agents such as dioxin in the Mozambique tilapia. The expression of three stress genes was examined: heat shock protein 90 (Hsp90), CYP1A1 as one of the main cytochrome P450 enzymes, and metallothionein (MT). The study exhibited a characteristic sensitivity to metal treatments. Liver samples were collected from all fish to analyze bio-indicators, including superoxide dismutase (SOD), malondialdehyde (MDA), and reactive oxygen species (ROS). While gills samples were used for DNA fragmentation assay. Results showed that oxidative stress in the dioxin group's liver significantly changed indicators. However, the dioxin group significantly increased the SOD, MDA enzyme activities, and ROS formation. Interestingly, the genes Hsp90, CYP1A1, and MT expression were significantly down-regulated in Dunaliella salina groups. Nevertheless, DNA fragmentation in gill organs was affected by exposure to dioxin in fish. Thus, it was concluded that the methanolic extract of an isolated strain Dunaliella salina is effective against mutagen agent dioxin by inhibiting genetic alterations in fish organs with an antioxidant defense system to conquer oxidative damage. |
| first_indexed | 2024-03-05T21:17:27Z |
| format | Article |
| id | utm.eprints-99610 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T21:17:27Z |
| publishDate | 2022 |
| publisher | Egyptian Society for the Development of Fisheries and Human Health |
| record_format | dspace |
| spelling | utm.eprints-996102023-03-05T08:35:57Z http://eprints.utm.my/99610/ The prevention impact of the green algal extract against genetic toxicity and antioxidant enzyme alteration in the Mozambique tilapia Khalil, Wagdy K. B. Bassem, Samah M. Sabry, Noha M. Abd. ElTawab, Marwa I. El Enshasy, Hesham Temraz, Tarek A. Guerriero, Giulia Abdel-Gawad, Fagr Kh. TP Chemical technology Algal studies are primary for ecological risk assessment and toxicology by evaluating lethal and sub-lethal toxic impacts of potential toxicants on inhabitants of numerous ecosystems. Dunaliella salina, a green marine alga, is characterized by its carotenoid accumulation and is widely used in many health and nutritional products. Our experiment was designed to evaluate algal extract's ability to inhibit genetic alterations induced by mutagen agents such as dioxin in the Mozambique tilapia. The expression of three stress genes was examined: heat shock protein 90 (Hsp90), CYP1A1 as one of the main cytochrome P450 enzymes, and metallothionein (MT). The study exhibited a characteristic sensitivity to metal treatments. Liver samples were collected from all fish to analyze bio-indicators, including superoxide dismutase (SOD), malondialdehyde (MDA), and reactive oxygen species (ROS). While gills samples were used for DNA fragmentation assay. Results showed that oxidative stress in the dioxin group's liver significantly changed indicators. However, the dioxin group significantly increased the SOD, MDA enzyme activities, and ROS formation. Interestingly, the genes Hsp90, CYP1A1, and MT expression were significantly down-regulated in Dunaliella salina groups. Nevertheless, DNA fragmentation in gill organs was affected by exposure to dioxin in fish. Thus, it was concluded that the methanolic extract of an isolated strain Dunaliella salina is effective against mutagen agent dioxin by inhibiting genetic alterations in fish organs with an antioxidant defense system to conquer oxidative damage. Egyptian Society for the Development of Fisheries and Human Health 2022 Article PeerReviewed application/pdf en http://eprints.utm.my/99610/1/HeshamElEnshasy2022_ThePreventionImpactoftheGreenAlgalExtract.pdf Khalil, Wagdy K. B. and Bassem, Samah M. and Sabry, Noha M. and Abd. ElTawab, Marwa I. and El Enshasy, Hesham and Temraz, Tarek A. and Guerriero, Giulia and Abdel-Gawad, Fagr Kh. (2022) The prevention impact of the green algal extract against genetic toxicity and antioxidant enzyme alteration in the Mozambique tilapia. Egyptian Journal of Aquatic Biology and Fisheries, 26 (5). pp. 1103-1118. ISSN 1110-6131 http://dx.doi.org/10.21608/ejabf.2022.266978 DOI : 10.21608/ejabf.2022.266978 |
| spellingShingle | TP Chemical technology Khalil, Wagdy K. B. Bassem, Samah M. Sabry, Noha M. Abd. ElTawab, Marwa I. El Enshasy, Hesham Temraz, Tarek A. Guerriero, Giulia Abdel-Gawad, Fagr Kh. The prevention impact of the green algal extract against genetic toxicity and antioxidant enzyme alteration in the Mozambique tilapia |
| title | The prevention impact of the green algal extract against genetic toxicity and antioxidant enzyme alteration in the Mozambique tilapia |
| title_full | The prevention impact of the green algal extract against genetic toxicity and antioxidant enzyme alteration in the Mozambique tilapia |
| title_fullStr | The prevention impact of the green algal extract against genetic toxicity and antioxidant enzyme alteration in the Mozambique tilapia |
| title_full_unstemmed | The prevention impact of the green algal extract against genetic toxicity and antioxidant enzyme alteration in the Mozambique tilapia |
| title_short | The prevention impact of the green algal extract against genetic toxicity and antioxidant enzyme alteration in the Mozambique tilapia |
| title_sort | prevention impact of the green algal extract against genetic toxicity and antioxidant enzyme alteration in the mozambique tilapia |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/99610/1/HeshamElEnshasy2022_ThePreventionImpactoftheGreenAlgalExtract.pdf |
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