Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip
Determination of feline meat in food products is an important issue for social, health, economic and religious concern. Hence this paper documented the application of species specific polymerase chain reaction-restriction fragment length polymorphism (SP-PCR-RFLP) assay targeting a short-fragments (...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier Ltd.
2020
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| Subjects: | |
| Online Access: | https://repo.uum.edu.my/id/eprint/27160/1/alamin2020.pdf |
| _version_ | 1803629256343814144 |
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| author | Amin, Md Al Rahman, Md Mahfujur Ahmad Razimi, Mohd Shahril Chowdhury, Zaira Zaman Md Hussain, Muhammad Nasri Mohd Desa, Mohd Nasir |
| author_facet | Amin, Md Al Rahman, Md Mahfujur Ahmad Razimi, Mohd Shahril Chowdhury, Zaira Zaman Md Hussain, Muhammad Nasri Mohd Desa, Mohd Nasir |
| author_sort | Amin, Md Al |
| collection | UUM |
| description | Determination of feline meat in food products is an important issue for social, health, economic and religious concern. Hence this paper documented the application of species specific polymerase chain reaction-restriction fragment length polymorphism (SP-PCR-RFLP) assay targeting a short-fragments (69 bp) of mitochondrial cytochrome b (cytb) gene to screen feline meat in commercial meat products using lab-on-a-chip. The SP-PCR assay proved its specificity theoretically and experimentally while testing with different common animal, aquatic and plant species of DNA. The feline specific (69 bp, 43- and 26-bp) characteristic molecular DNA pattern was observed by SP-PCR and RFLP analysis. For assay performance, it was tested in three different types of commercial dummy meat products such as frankfurters, nuggets and meatballs and digested with AluI-restriction enzyme. The highest sensitivity of the assay using lab-on-a-chip was as low as 0.1 pg or 0.01 % (w/w) in commercial dummy meat products. We have also applied this assay to screen three important commercial meat products of six different brand from six supermarket chains located at three different states of Malaysia. Thus total 378 samples were tested to validate the specificity, sensitivity, stability of the assay and utilization of it for commercial meat product screening. |
| first_indexed | 2024-07-04T06:34:58Z |
| format | Article |
| id | uum-27160 |
| institution | Universiti Utara Malaysia |
| language | English |
| last_indexed | 2024-07-04T06:34:58Z |
| publishDate | 2020 |
| publisher | Elsevier Ltd. |
| record_format | dspace |
| spelling | uum-271602020-07-01T07:03:43Z https://repo.uum.edu.my/id/eprint/27160/ Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip Amin, Md Al Rahman, Md Mahfujur Ahmad Razimi, Mohd Shahril Chowdhury, Zaira Zaman Md Hussain, Muhammad Nasri Mohd Desa, Mohd Nasir HB Economic Theory Determination of feline meat in food products is an important issue for social, health, economic and religious concern. Hence this paper documented the application of species specific polymerase chain reaction-restriction fragment length polymorphism (SP-PCR-RFLP) assay targeting a short-fragments (69 bp) of mitochondrial cytochrome b (cytb) gene to screen feline meat in commercial meat products using lab-on-a-chip. The SP-PCR assay proved its specificity theoretically and experimentally while testing with different common animal, aquatic and plant species of DNA. The feline specific (69 bp, 43- and 26-bp) characteristic molecular DNA pattern was observed by SP-PCR and RFLP analysis. For assay performance, it was tested in three different types of commercial dummy meat products such as frankfurters, nuggets and meatballs and digested with AluI-restriction enzyme. The highest sensitivity of the assay using lab-on-a-chip was as low as 0.1 pg or 0.01 % (w/w) in commercial dummy meat products. We have also applied this assay to screen three important commercial meat products of six different brand from six supermarket chains located at three different states of Malaysia. Thus total 378 samples were tested to validate the specificity, sensitivity, stability of the assay and utilization of it for commercial meat product screening. Elsevier Ltd. 2020 Article PeerReviewed application/pdf en https://repo.uum.edu.my/id/eprint/27160/1/alamin2020.pdf Amin, Md Al and Rahman, Md Mahfujur and Ahmad Razimi, Mohd Shahril and Chowdhury, Zaira Zaman and Md Hussain, Muhammad Nasri and Mohd Desa, Mohd Nasir (2020) Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip. Journal of Food Composition and Analysis, 92. pp. 1-32. ISSN 08891575 http://doi.org/10.1016/j.jfca.2020.103565 doi:10.1016/j.jfca.2020.103565 doi:10.1016/j.jfca.2020.103565 |
| spellingShingle | HB Economic Theory Amin, Md Al Rahman, Md Mahfujur Ahmad Razimi, Mohd Shahril Chowdhury, Zaira Zaman Md Hussain, Muhammad Nasri Mohd Desa, Mohd Nasir Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip |
| title | Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip |
| title_full | Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip |
| title_fullStr | Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip |
| title_full_unstemmed | Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip |
| title_short | Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip |
| title_sort | screening of commercial meat products from supermarket chains for feline derivatives using sp pcr rlfp and lab on a chip |
| topic | HB Economic Theory |
| url | https://repo.uum.edu.my/id/eprint/27160/1/alamin2020.pdf |
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