Properties of cloned ATP-sensitive K+ currents expressed in Xenopus oocytes. by Gribble, F, Ashfield, R, Ammälä, C, Ashcroft, F

“…We have studied the electrophysiological properties of cloned ATP-sensitive K+ channels (KATP channels) heterologously expressed in Xenopus oocytes. This channel comprises a sulphonylurea receptor subunit (SUR) and an inwardly rectifying K+ channel subunit (Kir). 2. …”

Expression of the cloned beta-cell ATP-sensitive potassium channel in Xenopus oocytes by Gribble, F, Ashfield, R, Ashcroft, F

Cloning and functional expression of the cDNA encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain. by Bond, C, Ammälä, C, Ashfield, R, Blair, T, Gribble, F, Khan, R, Lee, K, Proks, P, Rowe, I, Sakura, H

“…Heterologous expression in Xenopus oocytes produced currents which were K(+)-selective, time-independent and showed inward rectification. …”

Interaction of vanadate with the cloned beta cell K(ATP) channel. by Proks, P, Ashfield, R, Ashcroft, F

“…We explored the effect of vanadate (2 mM), in the absence and presence of magnesium nucleotides, on different types of cloned K(ATP) channels expressed in Xenopus oocytes. Currents were recorded from inside-out patches. …”

Identification of the high-affinity tolbutamide site on the SUR1 subunit of the K(ATP) channel. by Ashfield, R, Gribble, F, Ashcroft, S, Ashcroft, F

“…Chimeric SURs were coexpressed with Kir6.2 in Xenopus oocytes, and macroscopic currents were measured in inside-out membrane patches. …”

Nucleotide modulation of pinacidil stimulation of the cloned K(ATP) channel Kir6.2/SUR2A. by Gribble, F, Reimann, F, Ashfield, R, Ashcroft, F

“…To determine the reason for this anomaly, we examined the functional interactions between pinacidil (or P1075) and nucleotides by expressing cloned Kir6. 2/SUR2A channels in Xenopus laevis oocytes. Both pinacidil and P1075 activated macroscopic Kir6.2/SUR2A currents in the absence of added nucleotide, but the presence of intracellular ATP or ADP slowed the off-rate of the response. …”

The novel diazoxide analog 3-isopropylamino-7-methoxy-4H-1,2,4-benzothiadiazine 1,1-dioxide is a selective Kir6.2/SUR1 channel opener. by Dabrowski, M, Ashcroft, F, Ashfield, R, Lebrun, P, Pirotte, B, Egebjerg, J, Bondo Hansen, J, Wahl, P

“…We recorded cloned K(ATP) channel currents from inside-out patches excised from Xenopus laevis oocytes heterologously expressing Kir6.2/SUR1, Kir6.2/SUR2A, or Kir6.2/SUR2B, corresponding to the beta-cell, cardiac, and smooth muscle types of the K(ATP) channel. …”