Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus by Hamzah, Hairul Aini, Omar, Abdul Rahman, Bejo, Mohd Hair, Ideris, Aini

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Detection of avian leukosis virus using polymerase chain reaction and enzyme linked immunosorbent assay by Thapa Chhetri, Bala Ram, Abd Rasid, Omar, Md Mahfuzul Hoque, Arshad, Siti Suri, Bejo, Mohd Hair

“…The applications of polymerase chain reaction (PCR) and enzyme linked immunosorbent assay (ELISA) in detecting avian leukosis virus (ALV) subgroups A and J were studied in a flock of breeder chickens. …”
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Detection of infectious bursal disease virus using SYBR Green 1 based realtime polymerase chain reaction by Hamzah, Hairul Aini, Omar, Abdul Rahman, Bejo, Mohd Hair, Ideris, Aini

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Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method by Omar, Abdul Rahman, Bejo, Mohd Hair, Ideris, Aini, Hamzah, Hairul Aini

“…RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transriptase-polymerase chain reaction. Two different primer combinations (Primer IF & IVIR) and (Primer IF & RCLA) and real-time polymerase chain reaction conditions were designed and optimized for rapid differentiation of very virulent and vaccine strains of IBDV based on detection of signatory threshold cycle (Ct) and melting temperature (Tm) values. …”

Molecular differentiation of infectious Bursal Disease Virus IBDV) strains. by Omar, Abdul Rahman, Bejo, Mohd Hair, Ideris, Aini, Kong, Lih Ling

“…This invention relates to the detection and distinguishing Infectious Bursal Disease Virus (IBDV) strains by a fluorescent probe based real-time polymerase chain reaction in chicken or other birds. More particularly, this invention relates to distinguishing different Infectious Bursal Diseases Virus (IBDV) strains in chicken and other bird sample by novel subtype specific primers and fluorescent probe based on one-tube duplex Real-time Polymerase Chain Reaction (PCR) method. …”

Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation. by Hasoon, Mauida F., Mohd Daud, Hassan, Abdullah, Azila A., Arshad, Siti Suri, Bejo, Mohd Hair

“…The growth rate of these cells increased as the proportion of FBS increased from 2% to 20% at 25°C with optimum growth at the concentrations of 10% or 15% FBS. Polymerase chain reaction products were obtained from ASBB cells and tissues of sea bass with primer sets of microsatellite markers of sea bass. …”
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Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation by F. Hasoon, Mauida, Mohd Daud, Hassan, A. Abdullah, Azila, Arshad, Siti Suri, Bejo, Mohd Hair

“…The growth rate of these cells increased as the proportion of FBS increased from 2% to 20% at 25°C with optimum growth at the concentrations of 10% or 15% FBS. Polymerase chain reaction products were obtained from ASBB cells and tissues of sea bass with primer sets of microsatellite markers of sea bass. …”
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Prevalence of feline coronavirus in two cat populations in Malaysia by Sharif, Saeed, Arshad, Siti Suri, Bejo, Mohd Hair, Omar, Abdul Rahman, Allaudin, Zeenathul Nazariah, Ismail, Mohd Afzal Hafidz

“…RNA extracted from the faecal material was subjected to a reverse transcription-polymerase chain reaction (RT-PCR) using primers flanking for a conserved region of the virus genome. …”
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Tissue culture isolation, electron microscopic characterization and PCR-detection of a betanodavirus isolated from diseased Asian sea bass. by F. Hasoon, Mauida, Mohd. Daud, Hassan, Arshad, Siti Suri, Bejo, Mohd Hair

“…Identification using reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis of PCR product was conducted and gave a single PCR product at 460 bp in agarose gel, followed by nested PCR with specific primers for PCR products at 220 bp. …”
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Pathogenesis and diagnostic approaches of avian infectious bronchitis by Bande, Faruku, Arshad, Siti Suri, Omar, Abdul Rahman, Bejo, Mohd Hair, Abubakar, Muhammad Salisu, Abba, Yusuf

“…Meanwhile previous diagnostic methods such as serology and virus isolations are less sensitive and time consuming, respectively; current methods, such as reverse transcription polymerase chain reaction (RT-PCR), Restriction Fragment Length Polymorphism (RFLP), and sequencing, offer highly sensitive, rapid, and accurate diagnostic results, thus enabling the genotyping of new viral strains within the shortest possible time. …”
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Construction of a plasmid encoding chicken mitochondrial antiviral signaling (MAVS) gene by Mohamad Wali, Haryati Shila, Ideris, Aini, Omar, Abdul Rahman, Bejo, Mohd Hair

“…Prior to cloning, RNA extraction was carried out from chicken samples and converted to cDNA. Polymerase chain reaction (PCR) amplified product was ligated to the vector pJET1.2. …”
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Comparison of Sybr Green I, ELISA and conventional agarose gel-based PCR in the detection of infectious bursal disease virus by Hamzah, Hairul Aini, Omar, Abdul Rahman, Bejo, Mohd Hair, Ideris, Aini

“…The current available molecular method to detect infectious bursal disease virus (IBDV) is by reverse transcriptase-polymerase chain reaction (RT-PCR). However, the conventional PCR is time consuming, prone to error and less sensitive. …”
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Analysis of genetic variation of inducible nitric oxide synthase and natural resistance-associated macrophage protein 1 loci in Malaysian native chickens by Tohidi, Reza, Idris, Ismail, Panandam, Jothi Malar, Bejo, Mohd Hair

“…The genetic diversity of 100 Malaysian native chickens was investigated using polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP) for two candidate genes: inducible nitric oxide synthase (INOS) and natural resistance-associated macrophage protein 1 (NRAMP1). …”
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Towards developing commercialized real-time PCR kits for the detection of avian pathogens. by Omar, Abdul Rahman, Ideris, Aini, Bejo, Mohd Hair, Syed Hassan, Syarifah, Yusoff, Khatijah, Arshad, Siti Suri

“…Various formats of polymerase chain reaction (PCR) detection methods such as conventional/nested agarose, colorimetric and in situ have been developed and tested against important avian viruses. …”

Adaptation of infectious bronchitis virus in mammalian cell line by M. J., Suhairoslinda Hayati, Bejo, Mohd Hair, Arshad, Siti Suri, Omar, Abdul Rahman

“…The CPE was characterised by formation of syncytium, giant cell, dendritic shaped cell and finally plaque formation. The polymerase chain reaction (PCR) results confirmed the presence of IBV in the Vero cells at P2 and P3. …”
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Phylogenetic analysis of feline coronavirus isolates from healthy cats in Malaysia by Sharif, Saeed, Arshad, Siti Suri, Bejo, Mohd Hair, Omar, Abdul Rahman, Allaudin, Zeenathul Nazariah, Hafidz, Mohd Afzal

“…In present study, a 223 bp region of 3’UTR of four FCoVs isolated from healthy cats was amplified by a one-step reverse transcriptase polymerase chain reaction (RTPCR) and sequenced. The nucleotide sequences were aligned with those of FCoV reference strains and identity and phylogenetic tree were determined. …”
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Diagnostic methods for feline coronavirus: a review by Sharif, Saeed, Arshad, Siti Suri, Bejo, Mohd Hair, Omar, Abdul Rahman, Allaudin, Zeenathul Nazariah, Alazawy, Amer

“…Reverse transcriptase polymerase chain reaction (RT-PCR) has been used to detect FCoV and is rapid and sensitive, but results must be interpreted in the context of clinical findings. …”
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Molecular detection of Mycoplasma gallisepticum by real time PCR by Farhana Yasmin, Ideris, Aini, Omar, Abdul Rahman, Bejo, Mohd Hair, Tan, Sheau Wei, Tan, C. G., Islam, R., Ahmad, K.

“…Recently, real time polymerase chain reaction has been developed for the detection of infectious organisms, but so far only a limited number of diagnostic real time PCRs have been proposed for MG. …”
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Cloning and expression of equine influenza virus nonstructural gene in prokaryotic system by Abdulhadi, Abdulrahman Dahham, Fateh Mohamed, Bashir Ahmad, Omar, Abdul Rahman, Bejo, Mohd Hair

“…To obtain and develop an effective diagnostic method for equine influenza virus, the NS1 gene of H3N8 subtype was amplified by reverse transcriptase polymerase chain reaction (RT-PCR). Products were cloned into pCR2.0 using the TOPOTM TA cloning vector and subsequently sub-cloned into a prokaryotic expression vector, pRSET B. …”
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Molecular and pathological identification of feline coronavirus type I by Amer, Alazawy, Arshad, Siti Suri, Bejo, Mohd Hair, Omar, Abdul Rahman, Tengku Ibrahim, Tengku Azmi, Bande, Faruku, Ajwad, Assumaidaee

“…The result of virus isolation was confirmed using one step reverse transcription polymerase chain reaction (RT-PCR). To gain insight on the genetic variant of FCoV, the S-gene sequence was amplified using type 1 specific primers.Virus isolation showed cytopathic effect (CPE) characterized by giant cells, ballooning and cells detachment. …”
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