Multiple T cell expansions are found in the blood and synovial fluid of patients with reactive arthritis. by Allen, R, Gillespie, G, Hall, F, Edmonds, S, Hall, M, Wordsworth, B, McMichael, A, Bowness, P

“…OBJECTIVE: To look for evidence of T lymphocyte expansions in the blood and synovial fluid (SF) of patients with reactive arthritis (ReA). METHODS: Paired peripheral blood and synovial samples from 10 patients with ReA were studied by dual color flow cytometry using T cell receptor (TCR) V beta specific and CD4 or CD8 specific antibodies. …”

HLA-B27-RESTRICTED PEPTIDE PRESENTATION TO CYTOTOXIC T-CELLS IN REACTIVE ARTHRITIS by Bowness, P, Allen, R, Mcmichael, A, Edmunds, S, Hall, M, Bowman, S, Wordsworth, B

T-CELL RECEPTOR USAGE OF SYNOVIAL LYMPHOCYTES FROM A PATIENT WITH REACTIVE ARTHRITIS by Bowness, P, Wordsworth, B, Moss, P, Mcmichael, A, Bell, J

Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis. by Appel, H, Kuon, W, Kuhne, M, Wu, P, Kuhlmann, S, Kollnberger, S, Thiel, A, Bowness, P, Sieper, J

“…The application of HLA-B27 tetramers in HLA-B27-related diseases was performed with nine recently described Chlamydia-derived peptides in synovial fluid and peripheral blood, to examine the CD8+ T cell response against Chlamydia trachomatis antigens in nine patients with Chlamydia-triggered reactive arthritis (Ct-ReA). Four of six HLA-B27+ Ct-ReA patients had specific synovial T cell binding to at least one HLA-B27/Chlamydia peptide tetramer. …”

Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis. by Appel, H, Kuon, W, Kuhne, M, Wu, P, Kuhlmann, S, Kollnberger, S, Thiel, A, Bowness, P, Sieper, J

“…The application of HLA-B27 tetramers in HLA-B27-related diseases was performed with nine recently described Chlamydia-derived peptides in synovial fluid and peripheral blood, to examine the CD8+ T cell response against Chlamydia trachomatis antigens in nine patients with Chlamydia-triggered reactive arthritis (Ct-ReA). Four of six HLA-B27+ Ct-ReA patients had specific synovial T cell binding to at least one HLA-B27/Chlamydia peptide tetramer. …”

Conserved TCR beta chain usage in reactive arthritis; evidence for selection by a putative HLA-B27-associated autoantigen. by May, E, Dulphy, N, Frauendorf, E, Duchmann, R, Bowness, P, Lopez de Castro, J, Toubert, A, Märker-Hermann, E

“…Previous work suggested that expanded CD8+ T-cell clones in the synovial fluid (SF) of HLA-B27+ patients with reactive arthritis (ReA) preferentially use the T-cell receptor variable region (TCRBV) 1, similar CDR3 sequences, and joining region (BJ) 2S3. …”

Identification of HLA-B27-restricted peptides from the Chlamydia trachomatis proteome with possible relevance to HLA-B27-associated diseases. by Kuon, W, Holzhütter, H, Appel, H, Grolms, M, Kollnberger, S, Traeder, A, Henklein, P, Weiss, E, Thiel, A, Lauster, R, Bowness, P, Radbruch, A, Kloetzel, P, Sieper, J

“…The association of HLA-B27 with ankylosing spondylitis and reactive arthritis is the strongest one known between an MHC class I Ag and a disease. …”

Consistent patterns of expression of HLA class I free heavy chains in healthy individuals and raised expression in spondyloarthropathy patients point to physiological and pathologi... by Raine, T, Brown, D, Bowness, P, Hill Gaston, J, Moffett, A, Trowsdale, J, Allen, R

“…FHCs were present at higher levels in a reactive arthritis (ReA) population than in healthy controls and RA patients; differences not merely due to the presence of Human Leucocyte Antigen (HLA) B27. …”

HLA B27 in health and disease: a double-edged sword? by Bowness, P

“…First, expanded populations of T lymphocytes were found in both the blood and synovial fluid of patients with reactive arthritis (ReA). Secondly, a strong cytotoxic T-cell response to an HLA B27-restricted peptide epitope from Chlamydia trachomatis was found in a patient with ReA. …”

Salmonella exploits HLA-B27 and host unfolded protein responses to promote intracellular replication by Antoniou, A, Lenart, I, Kriston-Vizi, J, Iwawaki, T, Turmaine, M, McHugh, K, Ali, S, Blake, N, Bowness, P, Bajaj-Elliott, M, Gould, K, Nesbeth, D, Powis, S

“…OBJECTIVE:Salmonella enterica infections can lead to Reactive Arthritis (ReA), which can exhibit an association with human leucocyte antigen (HLA)-B*27:05, a molecule prone to misfolding and initiation of the unfolded protein response (UPR). …”