Evaluation of perfluorohexyloctane/polydimethylsiloxane for pancreas preservation for clinical islet isolation and transplantation by Sthle, M, Foss, A, Gustafsson, B, Lempinen, M, Lundgren, T, Rafael, E, Tufveson, G, Theisinger, B, Brandhorst, D, Korsgren, O, Friberg, A

“…Pancreas preservation using F6H8S5 proved as effective as UW and Custadiol when used within CIT up to 10 h, in terms of both isolation outcome and islet functionality. …”

Using HTK for prolonged pancreas preservation prior to human islet isolation by Caballero-Corbalán, J, Brandhorst, H, Malm, H, Felldin, M, Foss, A, Salmela, K, Tibell, A, Tufveson, G, Korsgren, O, Brandhorst, D

Using HTK for prolonged pancreas preservation prior to human islet isolation. by Caballero-Corbalán, J, Brandhorst, H, Malm, H, Felldin, M, Foss, A, Salmela, K, Tibell, A, Tufveson, G, Korsgren, O, Brandhorst, D

Long-term preservation of the pig pancreas by a one-layer method for successful islet isolation. by Brandhorst, D, Iken, M, Brendel, MD, Bretzel, R, Brandhorst, H

“…To clarify whether long-term pancreas preservation is feasible by this simplified procedure, pancreases from retired breeder pigs were subjected to 7-hour preservation utilizing PFC alone in a one-layer method (OLM, n = 8) or in combination with UW (TLM, n = 10). …”

Quality of Isolated Pig Islets is Improved Using Perfluorohexyloctane for Pancreas Storage in a Split Lobe Model by Brandhorst, H, Iken, M, Scott, W, Papas, K, Theisinger, B, Johnson, P, Korsgren, O, Brandhorst, D

“…Pancreas transportation between donor center and islet production facility is frequently associated with prolonged ischemia impairing islet isolation and transplantation outcomes. …”

Quality of isolated pig islets is improved using perfluorohexyloctane for pancreas storage in a split lobe model. by Brandhorst, H, Iken, M, Scott, W, Papas, K, Theisinger, B, Johnson, P, Korsgren, O, Brandhorst, D

“…Pancreas transportation between donor center and islet production facility is frequently associated with prolonged ischemia impairing islet isolation and transplantation outcomes. …”

Successful pancreas preservation by a perfluorocarbon-based one-layer method for subsequent pig islet isolation. by Brandhorst, D, Iken, M, Brendel, MD, Bretzel, R, Brandhorst, H

“… BACKGROUND: The oxygenation of human pancreas by the two-layer method (TLM) during cold storage was recently established for clinical islet transplantation. …”

Pig pancreas oxygenation at 20 degrees C increases islet ATP generation but deteriorates islet function. by Iken, M, Brandhorst, H, Korsgren, O, Brandhorst, D

“…Successful pancreas preservation during storage in oxygenated perfluorodecalin (PFD) is mainly related to oxidative ATP generation during storage. …”

The ratio between collagenase class I and class II influences the efficient islet release from the rat pancreas. by Brandhorst, H, Raemsch-Guenther, N, Raemsch, C, Friedrich, O, Huettler, S, Kurfuerst, M, Korsgren, O, Brandhorst, D

“…Because no information has been available, this study was aimed to investigate the isolation efficiency of different ratios between collagenase class II and I (C-ratio) in the rat pancreas serving as model for the human pancreas without being restricted by the large variability observed in human donors. …”

Influence of collagenase loading on long-term preservation of pig pancreas by the two-layer method for subsequent islet isolation. by Brandhorst, D, Iken, M, Tanioka, Y, Brendel, MD, Bretzel, R, Brandhorst, H

“… BACKGROUND: The introduction of the two-layer method (TLM) for long-term human pancreas preservation revealed the enormous potential of TLM to improve graft function of isolated islets. …”

Adaption of neutral protease activity for islet isolation from the long-term two-layer method-stored pig pancreas. by Brandhorst, D, Iken, M, Brendel, MD, Bretzel, R, Brandhorst, H

“…BACKGROUND: Islet release from the pancreas is mediated by both collagenase and neutral protease (NP), a critical effector of islet integrity. …”

Pancreas storage in oxygenated perfluorodecalin does not restore post-transplant function of isolated pig islets pre-damaged by warm ischemia. by Brandhorst, D, Iken, M, Bretzel, R, Brandhorst, H

“…CONCLUSIONS: The present study suggests that pancreas short-term storage in oxygenated PFD improves in vitro but not the in vivo function of ischemically damaged pig islets.…”

The ratio between class II and class I collagenase determines the amount of neutral protease activity required for efficient islet release from the rat pancreas. by Brandhorst, H, Alt, A, Huettler, S, Raemsch-Guenther, N, Kurfuerst, M, Bretzel, R, Brandhorst, D

“…Previous investigations clearly showed that the successful release of islets from the pancreas is mediated by both neutral protease (NP) and collagenase, consisting of subclasses I and II showing different capacities to cleave islets from the pancreas. …”

A new oxygen carrier for improved long-term storage of human pancreata before islet isolation. by Brandhorst, H, Asif, S, Andersson, K, Theisinger, B, Andersson, H, Felldin, M, Foss, A, Salmela, K, Tibell, A, Tufveson, G, Korsgren, O, Brandhorst, D

“… BACKGROUND: Pancreas oxygenation during cold storage has been established in islet isolation and transplantation to prevent ischemic tissue damage using perfluorodecalin (PFD) as hyperoxygen carrier. …”

Quantifying the effects of different neutral proteases on human islet integrity by Brandhorst, H, Johnson, P, Korsgren, O, Brandhorst, D

“…Efficient islet release from the pancreas requires the combination of collagenase, neutral protease (cNP), or thermolysin (TL). …”

The importance of tryptic-like activity in purified enzyme blends for efficient islet isolation. by Brandhorst, H, Friberg, A, Andersson, H, Felldin, M, Foss, A, Salmela, K, Lundgren, T, Tibell, A, Tufveson, G, Korsgren, O, Brandhorst, D

“… BACKGROUND: The isolation of islets from the human pancreas critically depends on an efficient enzyme blend. …”

Can the density of native pancreatic tissue slices predict human islet isolation and purification outcome? by Eckhard, M, Brendel, MD, Brandhorst, D, Brandhorst, H, Bretzel, R

“…INTRODUCTION: With currently available technology, the outcomes of human islet isolation and purification are still inconsistent, in part due to a lack of control of the pancreas donor and the procurement conditions. Using a single donor pancreas, the critical islet mass for establishing insulin independence of approximately 5000 engrafted islet equivalents (IEQ)/kg of recipient weight can only be retrieved from about one third of isolations. …”

New class of oxygen carriers improves islet isolation from long-term stored rat pancreata. by Brandhorst, H, Muehling, B, Yamaya, H, Henriksnaes, J, Carlsson, P, Korsgren, O, Brandhorst, D

“… OBJECTIVE: Pancreas shipment is frequently associated with prolonged ischemia deteriorating islet graft function. …”

Effect of pretransplant preconditioning by whole body hyperthermia on islet graft survival. by Brandhorst, H, Olbrich, M, Neumann, A, Jahr, H, Brandhorst, D

“…These findings suggest that WBH is primarily associated with increased necrosis as a secondary tissue type-specific effect of pancreas damage while in vitro HS mainly induces apoptosis.…”

Pancreatic L-Glutamine Administration Protects Pig Islets from Cold Ischemic Injury and Increases Resistance toward Inflammatory Mediators by Brandhorst, H, Theisinger, B, Guenther, B, Johnson, P, Brandhorst, D

“…In the present study we investigated whether pancreas oxygenation can be efficiently combined with an antioxidant strategy utilizing intraductal L-glutamine administration. …”