Molecular magnetic resonance imaging of acute vascular cell adhesion molecule-1 expression in a mouse model of cerebral ischemia. by Hoyte, L, Brooks, K, Nagel, S, Akhtar, A, Chen, R, Mardiguian, S, McAteer, M, Anthony, D, Choudhury, R, Buchan, A, Sibson, N

“…In this study, we show specific imaging of vascular cell adhesion molecule (VCAM)-1 expression in a mouse model of middle cerebral artery occlusion (MCAO), and a reduction in this inflammatory response, associated with improved behavioral outcome, as a result of preconditioning. …”

IN-VIVO MAGNETIC RESONANCE IMAGING OF RENAL ISCHAEMIA/REPERFUSION INJURY USING MICROPARTICLES OF IRON OXIDE TARGETING VASCULAR CELL ADHESION MOLECULE 1 by Akhtar, A, Schneider, J, McAteer, M, Chapman, S, Barnes, H, Digby, J, Wood, K, Choudhury, R

Targeted molecular imaging of vascular inflammation in cardiovascular disease using nano- and micro-sized agents by McAteer, M, Choudhury, R

“…We highlight strategies for detection of cell adhesion molecules, which are key regulators of endothelial activation and leukocyte recruitment in atherogenesis and ischaemia-reperfusion in jury. …”

Targeted molecular imaging of vascular inflammation in cardiovascular disease using nano- and micro-sized agents. by McAteer, M, Choudhury, R

“…We highlight strategies for detection of cell adhesion molecules, which are key regulators of endothelial activation and leukocyte recruitment in atherogenesis and ischaemia-reperfusion in jury. …”

Magnetic resonance imaging of endothelial adhesion molecules in mouse atherosclerosis using dual-targeted microparticles of iron oxide. by McAteer, M, Schneider, J, Ali, Z, Warrick, N, Bursill, C, von zur Muhlen, C, Greaves, D, Neubauer, S, Channon, K, Choudhury, R

“…We hypothesized that antibody-conjugated MPIO would enable magnetic resonance imaging (MRI) of endothelial cell adhesion molecules in mouse atherosclerosis. METHODS AND RESULTS: MPIO (4.5 microm) were conjugated to monoclonal antibodies against vascular cell adhesion molecule-1 (VCAM-MPIO) or P-selectin (P-selectin-MPIO). …”

Laser capture microdissection analysis of gene expression in macrophages from atherosclerotic lesions of apolipoprotein E-deficient mice. by Trogan, E, Choudhury, R, Dansky, H, Rong, J, Breslow, J, Fisher, E

“…To illustrate the ability of this method to measure changes in lesional macrophage gene expression, we injected 100 microg of lipopolysaccharide i.p. into apoE-deficient mice and detected in laser-captured lesional macrophages increased mRNA expression for vascular cell adhesion molecule-1, intercellular cell adhesion molecule-1, and monocyte chemoattractant protein-1 (11.9-, 32.5-, and 31.0-fold, respectively). …”

Chapter 4 - Applications of nanotechnology in molecular imaging of the brain. by McAteer, M, Choudhury, R

“…An array of iron oxide nano- and microparticle agents have been developed for in vivo molecular magnetic resonance imaging (mMRI) of cerebrovascular endothelial targets, such as vascular cell adhesion molecule-1 (VCAM-1), E-selectin, and the glycoprotein receptor GP IIb/IIIa expressed on activated platelets. …”

In vivo magnetic resonance imaging of acute brain inflammation using microparticles of iron oxide. by McAteer, M, Sibson, N, von Zur Muhlen, C, Schneider, J, Lowe, A, Warrick, N, Channon, K, Anthony, D, Choudhury, R

“…Endothelial vascular cell adhesion molecule-1 (VCAM-1) and its ligand, alpha4beta1 integrin, are key mediators of leukocyte recruitment, and selective inhibitors that bind to the alpha4 subunit of alpha4beta1 substantially reduce clinical relapse in multiple sclerosis. …”

Magnetic resonance imaging of brain inflammation using microparticles of iron oxide. by McAteer, M, von Zur Muhlen, C, Anthony, D, Sibson, N, Choudhury, R

“…This Chapter describes the methodology of a novel, functional MPIO probe targeting vascular cell adhesion molecule-1 (VCAM-1), for detection of acute brain inflammation in vivo, at a time when pathology is undetectable by conventional MRI. …”

An approach to molecular imaging of atherosclerosis, thrombosis, and vascular inflammation using microparticles of iron oxide. by McAteer, M, Akhtar, A, von Zur Muhlen, C, Choudhury, R

“…We have developed a range of novel ligand-conjugated MPIO for molecular MRI of endothelial adhesion molecules, such as vascular cell adhesion molecule-1 (VCAM-1) and P-selectin expressed in vascular inflammation, as well as activated platelet thrombosis. …”

High-density lipoproteins retard the progression of atherosclerosis and favorably remodel lesions without suppressing indices of inflammation or oxidation. by Choudhury, R, Rong, J, Trogan, E, Elmalem, V, Dansky, H, Breslow, J, Witztum, J, Fallon, J, Fisher, E

“…In nonlesional aorta, mRNA abundance for pro-inflammatory proteins (vascular cell adhesion molecule [VCAM]-1, intercellular adhesion molecule-1 [ICAM-1], monocyte chemoattractant protein-1 [MCP-1]) increased between 4 and 16 weeks in apoE-/- (but not wild-type) mice, and were not reduced by elevated HDL. …”

Molecular MRI enables early and sensitive detection of brain metastases. by Serres, S, Soto, MS, Hamilton, A, McAteer, M, Carbonell, W, Robson, M, Ansorge, O, Khrapitchev, A, Bristow, C, Balathasan, L, Weissensteiner, T, Anthony, D, Choudhury, R, Muschel, R, Sibson, N

“…We have developed a targeted MRI contrast agent based on microparticles of iron oxide that enables imaging of endothelial vascular cell adhesion molecule-1 (VCAM-1). Our objectives here were to determine whether VCAM-1 is up-regulated on vessels associated with brain metastases, and if so, whether VCAM-1-targeted MRI enables early detection of these tumors. …”

Anti-IL-17A treatment reduces clinical score and VCAM-1 expression detected by in vivo magnetic resonance imaging in chronic relapsing EAE ABH mice. by Mardiguian, S, Serres, S, Ladds, E, Campbell, S, Wilainam, P, McFadyen, C, McAteer, M, Choudhury, R, Smith, P, Saunders, F, Watt, G, Sibson, N, Anthony, D

“…We investigated the therapeutic effects of anti-IL-17A in a chronic relapsing EAE ABH mouse model using conventional scoring, quantitative behavioral outcomes, and a novel vascular cell adhesion molecule 1 (VCAM-1)-targeted magnetic resonance imaging (MRI) contrast agent [anti-VCAM-microparticles of iron oxide (MPIO)] to identify conventionally undetectable neuropathology. …”

Dietary glycotoxins promote diabetic atherosclerosis in apolipoprotein E-deficient mice. by Lin, R, Choudhury, R, Cai, W, Lu, M, Fallon, J, Fisher, E, Vlassara, H

“…Immunohistochemical comparisons showed markedly suppressed tissue AGE, AGE-Receptor-1, -2 and RAGE expression, reduced numbers of inflammatory cells, tissue factor, vascular cell adhesion molecule-1 and MCP-1 in the L-AGE diabetic group. …”

A leukocyte-mimetic magnetic resonance imaging contrast agent homes rapidly to activated endothelium and tracks with atherosclerotic lesion macrophage content by McAteer, M, Mankia, K, Ruparelia, N, Jefferson, A, Nugent, H, Stork, L, Channon, K, Schneider, J, Choudhury, R

“…We hypothesized that high-affinity dual-ligand microparticles of iron oxide (MPIO), targeted to P-selectin and vascular cell adhesion molecule-1 (PV-MPIO), would identify activated endothelial cells during atherosclerosis progression. …”

Endothelial cell-specific reactive oxygen species production increases susceptibility to aortic dissection. by Fan, L, Douglas, G, Bendall, J, McNeill, E, Crabtree, M, Hale, AB, Mai, A, Li, J, McAteer, M, Schneider, J, Choudhury, R, Channon, K

“…Nox2 transgenic aortas had increased endothelial ROS production, endothelial vascular cell adhesion molecule-1 expression, matrix metalloproteinase activity, and CD45(+) inflammatory cell infiltration. …”

Endothelial cell-specific reactive oxygen species production increases susceptibility to aortic dissection by Fan, L, Douglas, G, Bendall, J, McNeill, E, Crabtree, M, Hale, AB, Li, J, McAteer, M, Schneider, J, Choudhury, R, Channon, K

“…Nox2 transgenic aortas had increased endothelial ROS production, endothelial vascular cell adhesion molecule-1 expression, matrix metalloproteinase activity, and CD45+ inflammatory cell infiltration. …”

A leukocyte-mimetic magnetic resonance imaging contrast agent homes rapidly to activated endothelium and tracks with atherosclerotic lesion macrophage content. by McAteer, M, Mankia, K, Ruparelia, N, Jefferson, A, Nugent, H, Stork, L, Channon, K, Schneider, J, Choudhury, R

“…We hypothesized that high-affinity dual-ligand microparticles of iron oxide (MPIO), targeted to P-selectin and vascular cell adhesion molecule-1 (PV-MPIO), would identify activated endothelial cells during atherosclerosis progression. …”

In vivo quantification of VCAM-1 expression in renal ischemia reperfusion injury using non-invasive magnetic resonance molecular imaging. by Akhtar, A, Schneider, J, Chapman, S, Jefferson, A, Digby, J, Mankia, K, Chen, Y, McAteer, M, Wood, K, Choudhury, R

“…Vascular cell adhesion molecule-1 (VCAM-1) is upregulated in ischemia reperfusion injury (IRI), persisting after restoration of blood flow. …”

In vivo quantification of VCAM-1 expression in renal ischemia reperfusion injury using non-invasive magnetic resonance molecular imaging. by Akhtar, A, Schneider, J, Chapman, S, Jefferson, A, Digby, J, Mankia, K, Chen, Y, McAteer, M, Wood, K, Choudhury, R

“… RATIONALE AND OBJECTIVE: Vascular cell adhesion molecule-1 (VCAM-1) is upregulated in ischemia reperfusion injury (IRI), persisting after restoration of blood flow. …”