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Investigation of the effects of T-2 toxin in chicken-derived three-dimensional hepatic cell cultures
Published 2024-01-01“…To gain more accurate understanding, the effects of T-2 toxin were investigated in the present study in chicken-derived three-dimensional (3D) primary hepatic cell cultures. 3D spheroids were treated with three concentrations (100, 500, 1000 nM) of T-2 toxin for 24 h. Cellular metabolic activity declined in all treated groups as reflected by the Cell Counting Kit-8 assay, while extracellular lactate dehydrogenase activity was increased after 500 nM T-2 toxin exposure. …”
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Immunomodulatory effects of chicken cathelicidin-2 on a primary hepatic cell co-culture model.
Published 2022-01-01“…Both concentrations of cathelicidin-2 decreased the cellular metabolic activity and increased the extracellular lactate dehydrogenase (LDH) activity reflecting reduced membrane integrity. …”
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Immunomodulatory effects of chicken cathelicidin-2 on a primary hepatic cell co-culture model
Published 2022-01-01“…Both concentrations of cathelicidin-2 decreased the cellular metabolic activity and increased the extracellular lactate dehydrogenase (LDH) activity reflecting reduced membrane integrity. …”
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Luteolin: A Phytochemical to Mitigate <i>S.</i> Typhimurium Flagellin-Induced Inflammation in a Chicken In Vitro Hepatic Model
Published 2023-04-01“…Cells were cultured in a medium supplemented with 250 ng/mL flagellin and 4 or 16 µg/mL luteolin for 24 h. Cellular metabolic activity, lactate dehydrogenase (LDH) activity, interleukin-6, 8, 10 (IL-6, IL-8, IL-10), interferon-α, γ (IFN-α, IFN-γ), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and malondialdehyde (MDA) concentrations were determined. …”
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Two Sides to Every Question: Attempts to Activate Chicken Innate Immunity in 2D and 3D Hepatic Cell Cultures
Published 2021-07-01“…The cultures were challenged by the bacterial endotoxins lipopolysaccharide (LPS) from <i>Escherichia coli</i>, lipoteichoic acid (LTA) from <i>Staphylococcus aureus</i> and by enterotoxin (ETxB) from <i>Escherichia coli</i>, <i>Salmonella</i> Typhimurium derived flagellin, phorbol myristate acetate (PMA) as a model proinflammatory agent and polyinosinic polycytidylic acid (poly I:C) for mimicking viral RNA exposure. Cellular metabolic activity was assessed with the CCK-8 test, membrane damage was monitored with the lactate dehydrogenase (LDH) leakage assay and interleukin-6 and -8 (Il-6 and -8) concentrations were measured in cell culture medium with a chicken specific ELISA. …”
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