Pyridine nucleotide regulation of the KATP channel Kir6.2/SUR1 expressed in Xenopus oocytes. by Dabrowski, M, Trapp, S, Ashcroft, F

“…We therefore investigated whether NAD and NADP interact with both Kir6.2 and SUR1 subunits of the KATP channel by comparing the potency of these agents on recombinant Kir6.2DeltaC and Kir6.2/SUR1 channels expressed in Xenopus oocytes. Our results show that, at physiological concentrations, NAD and NADP interact with the nucleotide inhibitory site of Kir6.2 to inhibit Kir6.2/SUR1 currents. …”

Potent and selective activation of the pancreatic beta-cell type K(ATP) channel by two novel diazoxide analogues. by Dabrowski, M, Larsen, T, Ashcroft, F, Bondo Hansen, J, Wahl, P

“…METHODS: We studied the effects of these compounds on whole-cell currents through cloned K(ATP) channels expressed in Xenopus oocytes or mammalian cells (HEK293). We also used inside-out macropatches excised from Xenopus oocytes. …”

Effect of repaglinide on cloned beta cell, cardiac and smooth muscle types of ATP-sensitive potassium channels. by Dabrowski, M, Wahl, P, Holmes, W, Ashcroft, F

“…METHODS: The action of the drug was studied by whole-cell current recordings of KATP channels expressed either in Xenopus oocytes or mammalian cells (HEK293). We also used inside-out macropatches excised from Xenopus oocytes for detailed analysis of repaglinide action. …”

Characterization of two novel forms of the rat sulphonylurea receptor SUR1A2 and SUR1BDelta31. by Gros, L, Trapp, S, Dabrowski, M, Ashcroft, F, Bataille, D, Blache, P

“…Coinjection of SUR1A2 plus Kir6.2 into Xenopus oocytes or expression of a SUR1A2-Kir6.2 tandem in HEK-293 cells yielded large currents with characteristics similar to the wild-type K(ATP) channel. 3. …”

Mutations in the linker domain of NBD2 of SUR inhibit transduction but not nucleotide binding. by Matsuo, M, Dabrowski, M, Ueda, K, Ashcroft, F

“…We co-expressed Kir6.2 with wild-type or mutant SUR in Xenopus oocytes and recorded the resulting currents in inside-out macropatches. …”

Characterisation of new KATP-channel mutations associated with congenital hyperinsulinism in the Finnish population. by Reimann, F, Huopio, H, Dabrowski, M, Proks, P, Gribble, F, Laakso, M, Otonkoski, T, Ashcroft, F

“…METHODS: Wild type or mutant Kir6.2 and SUR1 subunits were coexpressed in Xenopus oocytes. The functional properties of the channels were examined by measuring currents in intact oocytes or giant inside-out membrane patches. …”

Analysis of the differential modulation of sulphonylurea block of beta-cell and cardiac ATP-sensitive K+ (K(ATP)) channels by Mg-nucleotides. by Reimann, F, Dabrowski, M, Jones, P, Gribble, F, Ashcroft, F

“…We examined the molecular basis of the different response of channels containing SUR1 and SUR2A, by recording currents from inside-out patches excised from Xenopus oocytes heterologously expressing wild-type or chimeric channels. …”

The novel diazoxide analog 3-isopropylamino-7-methoxy-4H-1,2,4-benzothiadiazine 1,1-dioxide is a selective Kir6.2/SUR1 channel opener. by Dabrowski, M, Ashcroft, F, Ashfield, R, Lebrun, P, Pirotte, B, Egebjerg, J, Bondo Hansen, J, Wahl, P

“…We recorded cloned K(ATP) channel currents from inside-out patches excised from Xenopus laevis oocytes heterologously expressing Kir6.2/SUR1, Kir6.2/SUR2A, or Kir6.2/SUR2B, corresponding to the beta-cell, cardiac, and smooth muscle types of the K(ATP) channel. …”