A boronic-acid-based probe for fluorescence polarization assays with penicillin binding proteins and β-lactamases by Inglis, SR, Strieker, M, Rydzik, A, Dessen, A, Schofield, C

A boronic-acid-based probe for fluorescence polarization assays with penicillin binding proteins and β-lactamases. by Inglis, SR, Strieker, M, Rydzik, A, Dessen, A, Schofield, C

“…We report the development of a fluorescence polarization (FP) assay for PBPs and "serine" β-lactamases using a boronic-acid-based, reversibly binding "tracer." The tracer was designed based on a crystal structure of a covalent complex between a boronic acid and PBP1b from Streptococcus pneumoniae. …”

Structure-guided design of cell wall biosynthesis inhibitors that overcome β-lactam resistance in Staphylococcus aureus (MRSA). by Contreras-Martel, C, Amoroso, A, Woon, E, Zervosen, A, Inglis, S, Martins, A, Verlaine, O, Rydzik, A, Job, V, Luxen, A, Joris, B, Schofield, C, Dessen, A

“…We have identified alkyl boronic acids that are active against pathogens including methicillin-resistant S. aureus (MRSA). …”