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Novel Anti-Fungal <span style="font-variant: small-caps">d</span>-Laminaripentaose-Releasing Endo-β-1,3-glucanase with a RICIN-like Domain from <i>Cellulosimicrobium funkei</i> HY-...
Published 2021-07-01“…The recombinant enzyme (rGluY: 57.5 kDa) displayed the highest degradation activity for laminarin at pH 4.5 and 40 °C, while the polysaccharide was maximally decomposed by its C-terminal truncated mutant enzyme (rGluYΔRICIN: 42.0 kDa) at pH 5.5 and 45 °C. The specific activity (26.0 U/mg) of rGluY for laminarin was 2.6-fold higher than that (9.8 U/mg) of rGluYΔRICIN for the same polysaccharide. …”
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Novel Bi-Modular GH19 Chitinase with Broad pH Stability from a Fibrolytic Intestinal Symbiont of <i>Eisenia fetida</i>, <i>Cellulosimicrobium funkei</i> HY-13
Published 2021-11-01“…The highest endochitinase activity of the recombinant enzyme (rChiJ: 30.0 kDa) toward colloidal shrimp shell chitin was found at pH 5.5 and 55 °C and was considerably stable in a wide pH range (3.5–11.0). …”
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Identification and Characterization of a Novel, Cold-Adapted <span style="font-variant: small-caps">d</span>-Xylobiose- and <span style="font-variant: small-caps">d</span>-Xylose-R...
Published 2021-04-01“…The recombinant enzyme (rXylR: 42.0 kDa) showed the highest beechwood xylan-degrading activity at pH 5.5 and 40 °C, and displayed 12% of its maximum activity even at 4 °C. rXylR was not only almost completely inhibited by 5 mM <i>N</i>-bromosuccinimide or metal ions (each 1 mM) including Hg<sup>2+</sup>, Ca<sup>2+</sup>, or Cu<sup>2+</sup> but also significantly suppressed by 1 mM Ni<sup>2+</sup>, Zn<sup>2+</sup>, or Fe<sup>2+</sup>. …”
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