Dynamic regulation and differential compartmentaliztion of C-type lectin receptors in the dendritic cell membrane by Luehr, J, Eissing, N, Heger, L, Lehmann, C, Heidkamp, G, Nimmerjahn, F, Balint, S, Sezgin, E, Eggeling, C, Dustin, M, Dudziak, D

Deficiency of lymphocyte function-associated antigen 3 (LFA-3) in paroxysmal nocturnal hemoglobinuria. Functional correlates and evidence for a phosphatidylinositol membrane anchor... by Selvaraj, P, Dustin, M, Silber, R, Low, MG, Springer, T

“…However, when LFA-3 purified from human E was reconstituted in sheep E or human E and subjected to PIPLC treatment, 40-50% of LFA-3 was released from the cell membrane. The results show that LFA-3 is attached to the cell membrane by a phosphatidylinositol glycolipid moiety, and confirm previous findings (37-41) that LFA-3 is a cell adhesion molecule that mediates adhesion by interacting with CD2 antigen.…”

Lateral membrane waves constitute a universal dynamic pattern of motile cells. by Döbereiner, H, Dubin-Thaler, B, Hofman, J, Xenias, H, Sims, T, Giannone, G, Dustin, M, Wiggins, C, Sheetz, M

“…Despite having different functions and being from multiple phyla, these cell types share a common spatiotemporal pattern in their normal membrane velocity; we show that protrusion and retraction events are organized in lateral waves along the cell membrane. These wave patterns indicate both spatial and temporal long-range periodic correlations of the actomyosin gel.…”

The immunological synapse. by Bromley, S, Burack, W, Johnson, K, Somersalo, K, Sims, T, Sumen, C, Davis, M, Shaw, A, Allen, P, Dustin, M

“…In this review we focus on the concept of immunological synapse formation as it relates to membrane structure, T cell polarity, signaling pathways, and the antigen-presenting cell. Membrane domains provide an organizational principle for compartmentalization within the immunological synapse. …”

The immune synapse: past, present, and future by Dustin, M, Baldari, C

“… <p style="text-align:justify;"> Immunological synapses are specialized cell-cell junctions characterized by (1) close apposition of the immune cell membrane with the membrane of another cell driven by adaptive or innate immune recognition, (2) adhesion, (3) stability, and (4) directed secretion. …”

Human immunodeficiency virus type 1 envelope gp120-induced partial T-cell receptor signaling creates an F-actin-depleted zone in the virological synapse. by Vasiliver-Shamis, G, Cho, M, Hioe, C, Dustin, M

“…We utilized supported planar bilayers presenting gp120 and ICAM-1 as a reductionist model for the infected-cell membrane and investigated its effect on the target CD4 T cell. …”

Micromanipulation of adhesion of phorbol 12-myristate-13-acetate-stimulated T lymphocytes to planar membranes containing intercellular adhesion molecule-1. by Tözeren, A, Mackie, L, Lawrence, M, Chan, P, Dustin, M, Springer, T

“…A micromanipulation method and mathematical analysis of cell deformation were used to determine (a) the area of conjugation between the cell and the substrate and (b) the energy that must be supplied to detach a unit area of the cell membrane from its substrate. T lymphocytes stimulated with phorbol 12-myristate-13-acetate (PMA) conjugated strongly with the planar membrane containing purified ICAM-1. …”

Motility and ultrastructure of large granular lymphocytes on lipid bilayers reconstituted with adhesion receptors LFA-1, ICAM-1, and two isoforms of LFA-3. by Carpén, O, Dustin, M, Springer, T, Swafford, J, Beckett, L, Caulfield, J

“…In the presence of T11(2/3) mAb, the area of cell membrane attaching to bilayers containing ICAM-1 and GPI-LFA-3 was decreased and the percentage of plasma membrane facing other cells was increased. …”

Cancer immunotherapy: Killers on sterols. by Dustin, M

“…In this issue, Yang and colleagues3 (page 651) propose a way to boost the function of antitumour T cells, using a metabolic trick to increase the level of cholesterol in the cells' membranes.…”

Making a little affinity go a long way: a topological view of LFA-1 regulation. by Dustin, M

“…Therefore it is important to determine mechanisms by which the properties of cell membranes and the cytoskeleton reinforce or hinder adhesion molecule interactions. …”

Polar redistribution of the sialoglycoprotein CD43: implications for T cell function. by Savage, N, Kimzey, S, Bromley, S, Johnson, K, Dustin, M, Green, J

“…This model predicts the topological seclusion of large molecules such as CD43 from the site of closest contact between the T cell and APC, allowing for the close apposition of cell membranes and effective TCR engagement. Similarly, during T cell migration segregation of CD43 to the uropod is thought to aid integrin adhesion at the leading edge of the cell by removing steric hindrance. …”