Influence of receptor lateral mobility on adhesion strengthening between membranes containing LFA-3 and CD2. by Chan, P, Lawrence, M, Dustin, M, Ferguson, L, Golan, D, Springer, T

“…Kinetic measurements demonstrated an influence of contact time on the strength of adhesion to the GPI isoform at lower site densities (25-50 sites/microns2), showing that the mobility of LFA-3 is important in adhesion strengthening. …”

Motility and ultrastructure of large granular lymphocytes on lipid bilayers reconstituted with adhesion receptors LFA-1, ICAM-1, and two isoforms of LFA-3. by Carpén, O, Dustin, M, Springer, T, Swafford, J, Beckett, L, Caulfield, J

“…Here we have studied the motility and ultrastructure of large granule lymphocyte (LGL) on lipid bilayers containing purified LFA-1, ICAM-1, and the transmembrane and glycophosphatidylinositol isoforms of LFA-3. LGLs moved at 8 microns/min on ICAM-1 but poorly (less than 1 microns/min) on its receptor pair LFA-1. …”

Signaling at neuro/immune synapses. by Dustin, M

“…The discovery of phosphatase micro-exclusion from signaling elements in immunological synapses and innate phagocytic synapses define a common functional unit at a common sub-micron scale across synapse types. Bundling of information from multiple antigen receptor microclusters by an immunological synapse has parallels to bundling of multiple synaptic inputs into a single axonal output by neurons, allowing integration and coincidence detection. …”

Signaling microdomains in T cells. by Choudhuri, K, Dustin, M

“…Sub-micron scale signaling domains induced in the plasma membrane of cells are thought to play important roles in signal transduction. …”

Membrane nanoclusters of FcγRI segregate from inhibitory SIRPα upon activation of human macrophages by Lopes, F, Bálint, Š, Valvo, S, Felce, J, Hessel, E, Dustin, M, Davis, D

“…Thus, a nanometer- and micron-scale reorganization of activating and inhibitory receptors occurs at the surface of human macrophages concurrent with signal integration.…”

TCR-mediated adhesion of T cell hybridomas to planar bilayers containing purified MHC class II/peptide complexes and receptor shedding during detachment. by Dustin, M, Miller, J, Ranganath, S, Vignali, D, Viner, N, Nelson, C, Unanue, E

“…T cell recognition of foreign Ag/MHC class II complexes is sensitive down to approximately 100 complexes per cell or approximately 0.2 complexes/micron2. To better understand the physical basis of the recognition stage of Ag presentation, we examined adhesion of the lysozyme- specific T cell hybridoma, 3A9, to artificial bilayers containing covalent MHC class II/peptide complexes or adhesion molecules. …”

Micromanipulation of adhesion of phorbol 12-myristate-13-acetate-stimulated T lymphocytes to planar membranes containing intercellular adhesion molecule-1. by Tözeren, A, Mackie, L, Lawrence, M, Chan, P, Dustin, M, Springer, T

“…The physical strength of adhesion between a PMA-stimulated T lymphocyte and a planar membrane containing 1,000 ICAM-1 molecules/micron 2 was comparable to the strength of adhesion between a cytotoxic T cell and its target cell. …”

Visualization of CD2 interaction with LFA-3 and determination of the two-dimensional dissociation constant for adhesion receptors in a contact area. by Dustin, M, Ferguson, L, Chan, P, Springer, T, Golan, D

“…The two-dimensional Kd for the CD2/LFA-3 interaction was 21 molecules/microns 2, which is lower than the surface densities of CD2 on T cells and LFA-3 on most target or stimulator cells. …”

Micromanipulation of adhesion of a Jurkat cell to a planar bilayer membrane containing lymphocyte function-associated antigen 3 molecules. by Tözeren, A, Sung, K, Sung, L, Dustin, M, Chan, P, Springer, T, Chien, S

“…In particular, we investigated the adhesion between a Jurkat cell expressing CD2 and a glass-supported planar bilayer containing either the glycosyl-phosphatidylinositol (GPI) or the transmembrane (TM) isoform of the counter-receptor lymphocyte function-associated antigen 3 (LFA-3) at a concentration of 1,000 molecules/microns 2. In response to the pipette force the Jurkat cells that adhered to the planar bilayer containing the GPI isoform of LFA-3 underwent extensive elongation. …”

Regulation of locomotion and cell-cell contact area by the LFA-1 and ICAM-1 adhesion receptors. by Dustin, M, Carpen, O, Springer, T

“…B lymphoblasts formed a large contact area and crawled rapidly (up to 25 microns/min) on planar bilayers bearing ICAM-1. In contrast, these cells attached to planar bilayers bearing LFA-1 through a fixed point about which the cells actively pivoted, using a single stalk-like projection. …”

Rosetting of human T lymphocytes with sheep and human erythrocytes. Comparison of human and sheep ligand binding using purified E receptor. by Selvaraj, P, Dustin, M, Mitnacht, R, Hünig, T, Springer, T, Plunkett, M

“…Human and sheep E have surface areas of 145 and 54 micron 2, respectively. The 3.2- to 5.6-fold higher ligand density on sheep E appears to account for the ability of sheep but not human E to rosette with certain types of human T lymphocytes.…”