Micropatterned comet assay enables high throughput and sensitive DNA damage quantification by Engelward, B. P., Ge, Jing, Chow, Danielle N., Fessler, Jessica L., Weingeist, David M., Wood, David K., Engelward, Bevin P.

“…To address these limitations, we created a microarray comet assay. The use of a micrometer scale array of cells increases the number of analysable comets per square centimetre and enables automated imaging and analysis. …”
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MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum by Xiong, Aoli, Kaushal, Simran, Tay, Ian Junjie, Engelward, Bevin P., Han, Jongyoon, Preiser, Peter Rainer

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High-Throughput Screening Platform for Engineered Nanoparticle-Mediated Genotoxicity Using CometChip Technology by Watson, Christa, Ge, Jing, Cohen, Joel, Pyrgiotakis, Georgios, Engelward, Bevin P., Demokritou, Philip

“…In this study, we present a high-throughput screening assay based upon the recently developed CometChip technology, which enables evaluation of single-stranded DNA breaks, abasic sites, and alkali-sensitive sites in cells exposed to ENPs. …”
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Novel In Vivo CometChip Reveals NDMA-Induced DNA Damage and Repair in Multiple Mouse Tissues by Owiti, Norah A., Corrigan, Joshua J., Pribyl, Lee J., Kay, Jennifer E., Engelward, Bevin P.

“…The comet assay is a versatile assay for detecting DNA damage in eukaryotic cells. …”
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CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells by Ge, Jing, Prasongtanakij, Somsak, Wood, David K., Weingeist, David M., Fessler, Jessica, Navasummrit, Panida, Ruchirawat, Mathuros, Engelward, Bevin P.

“…Here, we describe a novel platform that exploits microfabrication techniques to pattern cells in a fixed microarray The ‘CometChip’ is based upon the well-established single cell gel electrophoresis assay (a.k.a. the comet assay), which estimates the level of DNA damage by evaluating the extent of DNA migration through a matrix in an electrical field. …”
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Nitric oxide induced S-nitrosation causes base excision repair imbalance by Engelward, Bevin P., Parrish, Marcus Curtis, Chaim, Isaac Alexander, Nagel, Zachary D., Tannenbaum, Steven R, Samson, Leona D, Engelward, Bevin P

“…Furthermore, analysis of DNA damage using the CometChip (a higher throughput version of the comet assay) shows an increase in the levels of BER intermediates. …”
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Influenza infection induces host DNA damage and dynamic DNA damage responses during tissue regeneration by Li, Na, Yin, Lu, Rai, Prashant, Yoshiyuki, Yamada, Abolhassani, Nona, Kiraly, Orsolya, Chan, Tze Khee, Tan, Kong Bing, Chow, Vincent T. K., Engelward, Bevin P., Parrish, Marcus Curtis

“…We show that influenza induces DNA damage to both, when cells are directly exposed to virus in vitro (measured using the comet assay) and also when cells are exposed to virus in vivo (estimated via γH2AX foci). …”
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Standard fluorescent imaging of live cells is highly genotoxic by Ge, Jing, Wood, David K., Weingeist, David M., Prasongtanakij, Somsak, Navasummrit, Panida, Ruchirawat, Mathuros, Engelward, Bevin P.

“…To assess DNA damage, a high throughput platform for single cell gel electrophoresis is used (e.g., the CometChip). Light emitted by three standard filters was studied: (a) violet light [340–380 nm], used to excite DAPI and other blue fluorophores, (b) blue light [460–500 nm] commonly used to image green fluorescent protein (GFP) and Calcein AM, and (c) green light [528–553 nm], useful for imaging red fluorophores. …”
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