Downregulation of mGluR1-mediated signaling underlying autistic-like core symptoms in Shank1 P1812L-knock-in mice by Yue Qin, Xiao-Yong Zhang, Yanyan Liu, Zehan Ma, Shuo Tao, Ying Li, Rui Peng, Fei Wang, Jiucun Wang, Jianfeng Feng, Zilong Qiu, Li Jin, Hongyan Wang, Xiaohong Gong

“…To investigate the pathogenesis of the core symptoms in ASD, we constructed Shank1 P1812L-knock-in (KI) mice corresponding to a recurrent ASD-related mutation, SHANK1 P1806L, to achieve construct validity and face validity. …”
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Tet-dependent 5-hydroxymethyl-Cytosine modification of mRNA regulates axon guidance genes in Drosophila. by Badri Nath Singh, Hiep Tran, Joseph Kramer, Elmira Kirichenko, Neha Changela, Fei Wang, Yaping Feng, Dibyendu Kumar, Min Tu, Jie Lan, Martin Bizet, François Fuks, Ruth Steward

“…The identified mRNAs are preferentially involved in neuron and axon development and Tet knock-out led to a reduction of 5hmrC marks on specific mRNAs. …”
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Single-cell transcriptome analysis of epithelial, immune, and stromal signatures and interactions in human ovarian cancer by Chaochao Chai, Langchao Liang, Nanna S. Mikkelsen, Wei Wang, Wandong Zhao, Chengcheng Sun, Rasmus O. Bak, Hanbo Li, Lin Lin, Fei Wang, Yonglun Luo

“…Furthermore, the NECTIN2-TIGIT ligand-receptor pair was identified to mediate T cells communicating with epithelial, fibroblast, endothelial, and other cell types. Knock-out of NECTIN2 using CRISPR/Cas9 inhibited ovarian cancer cell (SKOV3) proliferation, and increased T cell proliferation when co-cultured. …”
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Melatonin alleviates alcoholic liver disease via EGFR–BRG1–TERT axis regulation by Zhaodi Che, Yali Song, Chengfang Xu, Wei Li, Zhiyong Dong, Cunchuan Wang, Yixing Ren, Kwok-Fai So, George L. Tipoe, Fei Wang, Jia Xiao

“…Biophysical assay found that epidermal growth factor receptor (EGFR) on the hepatocyte surface was a direct binding and regulating target of MLT. Liver specific knock-down of Tert or Egfr in the ALD mice model impaired MLT-mediated liver protection, partly through the regulation of nuclear brahma-related gene-1 (BRG1). …”
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Notch signaling mutations increase intra-tumor chemokine expression and predict response to immunotherapy in colorectal cancer by Fei Wang, Chuan Huang, Jie Long, Zhi-Bin Zhao, Hai-Qing Ma, Xue-Qing Yao, Liang Li, Zhe-Xiong Lian

“…We applied the Kaplan–Meier method for survival analysis in CRC patients who underwent immunotherapy, and the log-rank test to determine the statistically significant differences in survival. Notch1-knock-down cell line was constructed to detect the pathway and gene variations. …”
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TRIM25 inhibits HBV replication by promoting HBx degradation and the RIG-I-mediated pgRNA recognition by Hongxiao Song, Qingfei Xiao, Fengchao Xu, Qi Wei, Fei Wang, Guangyun Tan, Rongman Jia, Xiuyuan Hao

“…The TRIM25-mediated degradation of hepatitis B virus X (HBx) protein was determined by detecting the expression of HBx in TRIM25-overexpressed or knocked-out HepG2 or HepG2-NTCP cells via Western blotting. …”
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Long noncoding RNA NONMMUT015745 inhibits doxorubicin-mediated cardiomyocyte apoptosis by regulating Rab2A-p53 axis by Hongjing Cai, Pengchao Tian, Jie Ju, Tao Wang, Xinzhe Chen, Kai Wang, Fei Wang, Xue Yu, Shaocong Wang, Yin Wang, Chan Shan, Peifeng Li

“…Moreover, Rab2A, a direct target of lnc5745, possesses a protective effect in DOX-induced cardiotoxicity once knocked down. Importantly, we verified that the p53-related apoptotic signalling pathway was responsible for the lnc5745-mediated protective role against DOX-induced cardiomyocyte apoptosis. …”
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Baicalin inhibits the replication of the hepatitis B virus by targeting TRIM25 by Xixi Fan, Fei Wang, Hongxiao Song, Fengchao Xu, Xiaolu Li, Qi Wei, Bingxin Lei, Zhongnan Wang, Yue Wang, Guangyun Tan

“…Additionally, BA-mediated HBV inhibition was found to be significantly restored in HepG2 cells where TRIM25 was knocked out. Additional research into the mechanism of action of BA found that prolonged treatment with BA activated the JAK/STAT signaling pathway while simultaneously inhibiting the NF-kB pathway. …”
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Human INO80/YY1 chromatin remodeling complex transcriptionally regulates the BRCA2- and CDKN1A-interacting protein (BCCIP) in cells by Jiaming Su, Yi Sui, Jian Ding, Fuqiang Li, Shuang Shen, Yang Yang, Zeming Lu, Fei Wang, Lingling Cao, Xiaoxia Liu, Jingji Jin, Yong Cai

“…However, this enrichment is significantly inhibited by either knocking down INO80 or YY1, suggesting the existence of both INO80 and YY1 is required for recruiting the INO80/YY1 complex to BCCIP promoter region. …”
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Lipopolysaccharide Inhibits Autophagy and Promotes Inflammatory Responses via p38 MAPK-Induced Proteasomal Degradation of Atg13 in Hepatic Stellate Cells by Yuchao Wu, Yajuan He, Fei Wang, Naijuan Yao, Yingren Zhao, Zhen Tian

“…Secretion of IL-1β was assessed by ELISA. Atg13 was knocked down by siRNA in LX2 cells. Coimmunoprecipitation assay was used to detect protein binding and polyubiquitination of Atg13. …”
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Transcriptomic analyses of treatment-naïve pediatric ulcerative colitis patients and exploration of underlying disease pathogenesis by Xiaoli Pang, Hongxiao Song, Xiaolu Li, Fengchao Xu, Bingxun Lei, Fei Wang, Jing Xu, Lingli Qi, Libo Wang, Guangyun Tan

“…A CRISPR/Cas9 approach was used to knock out the expression of HLA-DRB5, and molecular biology techniques were used for additional mechanistic studies. …”
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Feedback Modulation between Human INO80 Chromatin Remodeling Complex and miR-372 in HCT116 Cells by Junaid Ali Shah, Yujuan Miao, Jinmeng Chu, Wenqi Chen, Qingzhi Zhao, Chengyu Cai, Saadullah Khattak, Fei Wang, Jingji Jin

“…Herein, we showed evidence that the INO80 complex transcriptionally controls microRNA-372 (miR-372) expression through RNA-Seq analysis and a series of biological experiments. Knocking down multiple subunits in the INO80 complex, including the <i>INO80</i> catalytic subunit, <i>YY1</i>, <i>Ies2</i>, and <i>Arp8</i>, can significantly increase the expression level of miR-372. …”
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