Efficient generation of Knock-in/Knock-out marmoset embryo via CRISPR/Cas9 gene editing by Kumita, Wakako, Sato, Kenya, Suzuki, Yasuhiro, Kurotaki, Yoko, Harada, Takeshi, Zhou, Yang, Kishi, Noriyuki, Sato, Kengo, Aiba, Atsu, Sakakibara, Yasubumi, Feng, Guoping, Okano, Hideyuki, Sasaki, Erika

“…Gene editing technologies have enabled production of target-gene knock-out (KO) NHP models. Target-gene-KO/knock-in (KI) efficiency of CRISPR/Cas9 has not been extensively investigated in marmosets. …”
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Impaired Dendritic Development and Memory in Sorbs2 Knock-Out Mice by Feliciano, C., Itohara, S., Dong, X., Zhang, Qiangge, Gao, Xian, Li, Chenchen, Wang, Dongqing, Zhou, Dingxi, Mei, Yuan, Monteiro, Patricia, Anand, Michelle S., Fu, Zhanyan, Feng, Guoping

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Tmem119-EGFP and Tmem119-CreERT2 Transgenic Mice for Labeling and Manipulating Microglia by Kaiser, Tobias, Feng, Guoping

“…We have targeted the recently discovered microglia-specific Tmem119 gene to generate knock-in mice expressing EGFP (JAX#031823) or CreERT2 (JAX#031820) for the identification and manipulation of microglia, respectively. …”
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An Ultra-Sensitive Step-Function Opsin for Minimally Invasive Optogenetic Stimulation in Mice and Macaques by Gong, Xin, Mendoza-Halliday, Diego, Ting, Jonathan T, Kaiser, Tobias, Sun, Xuyun, Bastos, André M, Wimmer, Ralf D, Guo, Baolin, Chen, Qian, Zhou, Yang, Pruner, Maxwell, Wu, Carolyn W-H, Park, Demian, Deisseroth, Karl, Barak, Boaz, Boyden, Edward S, Miller, Earl K, Halassa, Michael M, Fu, Zhanyan, Bi, Guoqiang, Desimone, Robert, Feng, Guoping

“…We show that SOUL can activate neurons located in deep mouse brain regions via transcranial optical stimulation and elicit behavioral changes in SOUL knock-in mice. Moreover, SOUL can be used to modulate neuronal spiking and induce oscillations reversibly in macaque cortex via optical stimulation from outside the dura. …”
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An Ultra-Sensitive Step-Function Opsin for Minimally Invasive Optogenetic Stimulation in Mice and Macaques by Gong, Xin, Mendoza-Halliday, Diego, Ting, Jonathan T., Kaiser, Tobias, Sun, Xuyun, Bastos, André M., Wimmer, Ralf D., Guo, Baolin, Chen, Qian, Zhou, Yang, Pruner, Maxwell, Wu, Carolyn W.-H., Park, Demian, Deisseroth, Karl, Barak, Boaz, Boyden, Edward S., Miller, Earl K., Halassa, Michael M., Fu, Zhanyan, Bi, Guoqiang, Desimone, Robert, Feng, Guoping

“…We show that SOUL can activate neurons located in deep mouse brain regions via transcranial optical stimulation and elicit behavioral changes in SOUL knock-in mice. Moreover, SOUL can be used to modulate neuronal spiking and induce oscillations reversibly in macaque cortex via optical stimulation from outside the dura. …”
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Adult restoration of Shank3 expression rescues selective autistic-like phenotypes by Mei, Yuan, Monteiro, Patricia, Zhou, Yang, Kim, Jinah, Gao, Xian, Fu, Zhanyan, Feng, Guoping

“…We generated a novel Shank3 conditional knock-in mouse model, and show that re-expression of the Shank3 gene in adult mice led to improvements in synaptic protein composition, spine density and neural function in the striatum. …”
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Effects of a patient-derived de novo coding alteration of CACNA1I in mice connect a schizophrenia risk gene with sleep spindle deficits by Ghoshal, Ayan, Uygun, David S, Yang, Lingling, McNally, James M, Lopez-Huerta, Violeta G, Arias-Garcia, Mario A, Baez-Nieto, David, Allen, Andrew, Fitzgerald, Megan, Choi, Soonwook, Zhang, Qiangge, Hope, Jen M, Yan, Karena, Mao, Xiaohong, Nicholson, Thomas B, Imaizumi, Kazuo, Fu, Zhanyan, Feng, Guoping, Brown, Ritchie E, Strecker, Robert E, Purcell, Shaun M, Pan, Jen Q

“…Here, we generated CaV3.3-RH knock-in animals, along with mice lacking CaV3.3, to investigate the biological impact of R1346H (RH) variation. …”
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Effects of a patient-derived de novo coding alteration of CACNA1I in mice connect a schizophrenia risk gene with sleep spindle deficits by Ghoshal, Ayan, Uygun, David S., Yang, Lingling, McNally, James M., Lopez-Huerta, Violeta G., Arias-Garcia, Mario A., Baez-Nieto, David, Allen, Andrew, Fitzgerald, Megan, Choi, Soonwook, Zhang, Qiangge, Hope, Jen M., Yan, Karena, Mao, Xiaohong, Nicholson, Thomas B., Imaizumi, Kazuo, Fu, Zhanyan, Feng, Guoping, Brown, Ritchie E., Strecker, Robert E., Purcell, Shaun M., Pan, Jen Q.

“…Here, we generated CaV3.3-RH knock-in animals, along with mice lacking CaV3.3, to investigate the biological impact of R1346H (RH) variation. …”
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Sapap3 Deletion Anomalously Activates Short-Term Endocannabinoid-Mediated Synaptic Plasticity by Chen, Meng, Wan, Yehong, Ade, Kristen, Ting, Jonathan, Feng, Guoping, Calakos, Nicole

“…SAP90/PSD-95-associated proteins (SAPAPs) are a family of postsynaptic proteins unique to excitatory synapses. Using Sapap3 knock-out (KO) mice, we find that, in the absence of SAPAP3, striatal medium spiny neuron (MSN) excitatory synapses exhibit eCB-mediated synaptic depression under conditions that do not normally activate this process. …”
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Microglia and Myelin: Improved Tools and Molecular Interactions by Kaiser, Tobias

“…Harnessing the microglia-specific Tmem119 locus and CRISPR/Cas, I engineered knock-in mice expressing EGFP or CreERT2 and show that these lines are highly specific in discerning microglia from other closely related myeloid cells. …”
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