Nitric oxide modulates superoxide release and peroxynitrite formation in human blood vessels. by Guzik, T, West, N, Pillai, R, Taggart, D, Channon, K

“…Accordingly, we systematically measured NO, superoxide, and peroxynitrite production from human internal mammary arteries, radial arteries, and saphenous veins from 78 patients undergoing coronary bypass surgery. Basal superoxide release was detected in all vessels at similar levels. …”

Functional estrogen receptor alpha promoter polymorphism is associated with improved endothelial-dependent vasolidation. by Figtree, G, Guzik, T, Robinson, BG, Channon, K, Watkins, H

“…Endothelial-dependent relaxation (EDR) was measured in organ bath preparations of human saphenous vein obtained from 101 individuals (81 males and 20 females) undergoing coronary artery bypass surgery. The presence of the variant C allele was associated with enhanced EDR independently of hypercholesterolaemia, smoking and diabetes, as well as sex (ANOVA for ACh induced relaxation: p=0.033). …”

Mechanisms of increased vascular superoxide production in human diabetes mellitus: role of NAD(P)H oxidase and endothelial nitric oxide synthase. by Guzik, T, Mussa, S, Gastaldi, D, Sadowski, J, Ratnatunga, C, Pillai, R, Channon, K

“…METHODS AND RESULTS: Vascular superoxide production was quantified in both saphenous veins and internal mammary arteries from 45 diabetic and 45 matched nondiabetic patients undergoing coronary artery bypass surgery. NAD(P)H-dependent oxidases were important sources of vascular superoxide in both diabetic and nondiabetic patients, but both the activity of this enzyme system and the levels of NAD(P)H oxidase protein subunits (p22phox, p67phox, and p47phox) were significantly increased in diabetic veins and arteries. …”

Systemic regulation of vascular NAD(P)H oxidase activity and nox isoform expression in human arteries and veins. by Guzik, T, Sadowski, J, Kapelak, B, Jopek, A, Rudzinski, P, Pillai, R, Korbut, R, Channon, K

“…METHODS AND RESULTS: We compared endothelial function, vascular superoxide production, and the expression of NAD(P)H oxidase subunits in arteries and veins from patients undergoing coronary bypass surgery (n=86). Superoxide release was similar in arteries and veins. …”

Shear stress insensitivity of endothelial nitric oxide synthase expression as a genetic risk factor for coronary heart disease. by Cattaruzza, M, Guzik, T, Słodowski, W, Pelvan, A, Becker, J, Halle, M, Buchwald, AB, Channon, K, Hecker, M

“…Moreover, NO-dependent relaxation was examined in segments of saphenous vein isolated from genotyped patients undergoing aortocoronary bypass surgery, and patients subjected to quantitative coronary angiography were genotyped to verify an association between this SNP and CHD. …”

Relationship of NAD(P)H oxidase activity in human blood vessels to endothelial dysfunction and clinical risk factors by Guzik, T, West, N, Black, E, McDonald, D, Ratnatunga, C, Pillai, R, Channon, K

“…Methods: We used human saphenous vein (HSV) and internal mammary artery (IMA) segments from patients undergoing coronary bypass surgery, to study: (1) Basal and NAD(P)H-stimulated superoxide production, using lucigenin-enhanced chemiluminescence. (2) NO-dependent vasorelaxations, as a bioassay of NO production. (3) Immunochemistry to show endothelial cells and eNOS protein. …”