Dopaminergic Signaling in the Cochlea: Receptor Expression Patterns and Deletion Phenotypes by Maison, Stephane F., Liu, Xiao-Ping, Eatock, Ruth Anne, Sibley, David R., Grandy, David K., Liberman, M. Charles

“…The increased vulnerability in D2 knock-outs was seen in DPOAEs, suggesting a role for dopamine in the outer hair cell area. …”
Get full text

Characterization of FUS Mutations in Amyotrophic Lateral Sclerosis Using RNA-Seq by van Blitterswijk, Marka, Wang, Eric T., Friedman, Brad Aaron, Keagle, Pamela J., Lowe, Patrick, Leclerc, Ashley Lyn, van den Berg, Leonard H., Housman, David E., Veldink, Jan H., Landers, John E.

“…Pathway analysis revealed that overexpression of wild-type FUS regulates ribosomal genes, whereas knock-down of FUS additionally affects expression of spliceosome related genes. …”
Get full text
Get full text

Aberrant Development and Plasticity of Excitatory Visual Cortical Networks in the Absence of cpg15 by Picard, Nathalie, Leslie, Jennifer H., Trowbridge, Sara Kishi, Subramanian, Jaichandar, Nedivi, Elly, Fagiolini, Michela

“…Here we show that global knock-out of cpg15 results in abnormal postnatal development of the excitatory network in visual cortex and an associated disruption in development of visual receptive field properties. …”
Get full text
Get full text

Using Topology of the Metabolic Network to Predict Viability of Mutant Strains by Mirny, Leonid A., Wunderlich, Zeba

“…In contrast, approaches utilizing both the topology and biochemical parameters of metabolic networks, e.g. flux balance analysis (FBA), are more successful in predicting phenotypes of knock-out strains. Results: We reconcile these seemingly conflicting results by showing that the topology of E. coli's metabolic network is, in fact, sufficient to predict the viability of knock-out strains with accuracy comparable to FBA on a large, unbiased dataset of mutants. …”
Get full text
Get full text

Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease by Joung, Julia, Zhang, Feng, Konermann, Silvana, Dahlman, James E., Abudayyeh, Omar Osama, Gootenberg, Jonathan S.

“…We have developed a CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell. …”
Get full text
Get full text
Get full text
Get full text
Get full text

Phosphomimetic Modulation of eNOS Improves Myocardial Reperfusion and Mimics Cardiac Postconditioning in Mice by Pong, Terrence, Scherrer-Crosbie, Marielle, Atochin, Dmitriy N., Bloch, Kenneth D., Huang, Paul L.

“…Reperfusion after myocardial ischemia-reperfusion injury is improved by postconditioning, as well as by phosphomimetic eNOS modulation. Knock-in mice expressing a phosphomimetic S1176D form of eNOS showed improved myocardial reperfusion and significantly reduced infarct size. eNOS knock-out mice failed to show cardioprotection from postconditioning. …”
Get full text

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector by Shmakov, S., Makarova, K. S., Semenova, E., Minakhin, L., Severinov, K., Koonin, E. V., Regev, Aviv, Gootenberg, Jonathan S, Konermann, Silvana M, Joung, Julia, Slaymaker, Ian, Cox, David Benjamin Turitz, Lander, Eric Steven, Zhang, Feng, Abudayyeh, Omar O.

“…In bacteria, C2c2 can be programmed to knock down specific mRNAs. Cleavage is mediated by catalytic residues in the two conserved Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains, mutations of which generate catalytically inactive RNA-binding proteins. …”
Get full text
Get full text
Get full text
Get full text
Get full text
Get full text
Get full text
Get full text

Rational Design of a Biomimetic Cell Penetrating Peptide Library by Urbanska, Aleksandra M., Sahay, Gaurav, Jhunjhunwala, Siddharth, Karagiannis, Emmanouil, Anderson, Daniel Griffith, Pelet, Jeisa, Langer, Robert S

“…Three human peptides derived from surfactant protein B (a lung surfactant protein), orexin (a neuropeptide hormone, and lactoferricin (a globular glycoprotein) that exist in many physiological fluids facilitated the in vivo delivery of siRNA and induce significant knock down (90%) of a hepatocyte expressed protein, coagulation Factor VII.…”
Get full text
Get full text
Get full text
Get full text
Get full text
Get full text

Exogenous delivery of chaperonin subunit fragment ApiCCT1 modulates mutant Huntingtin cellular phenotypes by Sontag, Emily M., Joachimiak, Lukasz A., Tan, Zhiqun, Tomlinson, Anthony, Glabe, Charles G., Potkin, Steven G., Frydman, Judith, Thompson, Leslie M., Housman, David E

“…ApiCCT1r reduces mHtt-mediated toxicity in immortalized striatal cells derived from full-length knock-in HD mice, suggesting that therapeutic benefit may extend beyond effects on aggregation. …”
Get full text
Get full text

Polymeric Nanoparticle PET/MR Imaging Allows Macrophage Detection in Atherosclerotic Plaques by Majmudar, Maulik D., Yoo, J., Keliher, Edmund J., Truelove, J. J., Iwamoto, Y., Sena, B., Dutta, P., Borodovsky, Anna, Fitzgerald, K., Di Carli, M. F., Libby, Peter, Swirski, F. K., Weissleder, R., Nahrendorf, Matthias, Anderson, Daniel Griffith

Get full text
Get full text