The attachments of chromatin loops to the nucleoskeleton. by Jackson, D, Dolle, A, Robertson, G, Cook, P

“…It is widely assumed by cell biologists that chromatin is looped by attachment to some nuclear skeleton. 'Structural' attachments might be mediated through specific sequences; these would be attached in most cells in an organism, underlying the basic structure of the mitotic chromosome and persisting throughout interphase. …”

Visualization of replication factories attached to nucleoskeleton. by Hozák, P, Hassan, A, Jackson, D, Cook, P

Attachment of repeated sequences to the nuclear cage. by Jackson, D, Cook, P, Patel, S

“…However no "attachment sequence" could be identified.…”

REPLICATION AND TRANSCRIPTION DEPEND ON ATTACHMENT OF DNA TO THE NUCLEAR CAGE by Jackson, D, Mccready, S, Cook, P

Transcriptionally active minichromosomes are attached transiently in nuclei through transcription units. by Jackson, D, Cook, P

“…Viral minichromosomes provide simple models for chromatin domains. The sequences attaching them to larger nuclear structures were mapped; attachments were defined operationally by their ability to prevent chromatin fragments electroeluting from nuclei. …”

Attachment of DNA to the nucleoskeleton of HeLa cells examined using physiological conditions. by Jackson, D, Dickinson, P, Cook, P

“…Although it is widely believed that eukaryotic DNA is looped by attachment to a nucleoskeleton, there is controversy about its composition and which sequences are attached to it. …”

Sequences attaching loops of nuclear and mitochondrial DNA to underlying structures in human cells: the role of transcription units. by Jackson, D, Bartlett, J, Cook, P

“…As chromatin is prone to rearrangement, care was taken to isolate sequences using 'physiological' conditions that did not create additional attachments. Comparison (by Southern blotting) of the concentration of each cloned sequence in 'total' and 'attached' fractions of DNA showed that most clones did contain attached sequences, but even highly-attached sequences were not attached in all cells in the population. …”

Active RNA polymerase I is fixed within the nucleus of HeLa cells. by Dickinson, P, Cook, P, Jackson, D

“…We have investigated whether active RNA polymerase I, the enzyme responsible for transcribing ribosomal RNA, is immobilized by attachment to a large subnuclear structure in HeLa cells. …”

Active RNA polymerases are localized within discrete transcription "factories' in human nuclei. by Iborra, F, Pombo, A, Jackson, D, Cook, P

“…Analogues were concentrated in approximately 2,100 (range 2,000-2,700) discrete sites attached to a nucleoskeleton and surrounded by chromatin. …”

Sites in human nuclei where DNA damaged by ultraviolet light is repaired: visualization and localization relative to the nucleoskeleton. by Jackson, D, Balajee, A, Mullenders, L, Cook, P

“…However, the procedure destroyed repair activity, so repaired DNA might be attached in vivo through a polymerase that was removed electrophoretically. …”

The structural basis of nuclear function. by Jackson, D, Cook, P

“…We review here experiments that suggest an alternative in which polymerization occurs as the template slides past a polymerase fixed to a large structure in the eukaryotic nucleus--a "factory" attached to a nucleoskeleton. This means that higher-order structure dictates how and when DNA is replicated or transcribed.…”

A view through the clouds of imprinting. by Burns, J, Jackson, D, Hassan, A

“…We have approached the problem of imprinting using a model based on the dynamic attachment of chromatin loops to immobilized RNA polymerases and control elements. …”

The topology of transcription by immobilized polymerases. by Iborra, F, Pombo, A, McManus, J, Jackson, D, Cook, P

“…Locus controlling regions and enhancers would attach to a factory and increase the chances that a promoter could bind to a polymerase; after transcriptional termination, the gene would detach from the factory. …”

A general method for preparing chromatin containing intact DNA. by Jackson, D, Cook, P

“…We illustrate the usefulness of this chromatin in investigations on the attachment of nascent RNA to the nucleoskeleton, the accessibility of the ribosomal locus to EcoRI and the properties of the endogenous RNA polymerase II. …”

Replication factories and nuclear bodies: the ultrastructural characterization of replication sites during the cell cycle. by Hozák, P, Jackson, D, Cook, P

“…Electron microscopy of thick resinless sections from which approximately 90% chromatin had been removed showed that most DNA synthesis occurs in specific dense structures (replication factories) attached to a diffuse nucleoskeleton. These factories appear at the end of G1-phase and quickly become active; as S-phase progresses, they increase in size and decrease in number like sites of incorporation seen by light microscopy. …”

Replication occurs at a nucleoskeleton. by Jackson, D, Cook, P

“…Since chromatin particles containing DNA the size of 125 kbp can electroelute, we conclude that the polymerizing complex is attached to a nucleoskeleton which is too large to escape. …”

The identification of a new alternative exon with highly restricted tissue expression in transcripts encoding the mouse Pgp-1 (CD44) homing receptor. Comparison of all 10 variable... by Screaton, G, Bell, M, Bell, J, Jackson, D

“…The human CD44 cell surface glycoprotein family which has been implicated in lymphocyte homing, tumor metastasis, and extracellular matrix attachment consists of a large number of related isoforms that derive from the differential splicing of a single CD44 gene transcript. …”

Numbers and organization of RNA polymerases, nascent transcripts, and transcription units in HeLa nuclei. by Jackson, D, Iborra, F, Manders, E, Cook, P

“…These results suggest that many templates are attached in a "cloud" of loops around a site; each site, or transcription "factory," would contain approximately 30 active polymerases and associated transcripts.…”

CD44H expression by human neuroblastoma cells: relation to MYCN amplification and lineage differentiation. by Gross, N, Beretta, C, Peruisseau, G, Jackson, D, Simmons, D, Beck, D

“…The human CD44 cell surface glycoprotein has been involved in a variety of functions including lymphocyte homing, extracellular cell matrix attachment, and tumor metastasis. Due to the alternative splicing of the single gene, a large family of different variants or isoforms is generated. …”

Numbers and organization of RNA polymerases, nascent transcripts, and transcription units in HeLa nuclei by Jackson, D, Iborra, F, Manders, E, Cook, P

“…These results suggest that many templates are attached in a "cloud" of loops around a site; each site, or transcription "factory," would contain ~30 active polymerases and associated transcripts. …”