Establishment of an Integrated CRISPR/Cas9 Plasmid System for Simple and Efficient Genome Editing in Medaka In Vitro and In Vivo by Zeming Zhang, Jie Wang, Jianeng Li, Xiang Liu, Lei Liu, Changle Zhao, Wenjing Tao, Deshou Wang, Jing Wei

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RFX1 participates in doxorubicin‐induced hepatitis B virus reactivation by Jie Wang, Junqiao Jia, Ran Chen, Shanlong Ding, Qiang Xu, Ting Zhang, Xiangmei Chen, Shuang Liu, Fengmin Lu

“…Furthermore, the activity of doxorubicin in promoting HBV replication was significantly attenuated when the expression of endogenous RFX1 was knocked down, and the EP element of HBV enhancer I, an element that mediated the binding of RFX1 and HBV enhancer I, was mutated. …”
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Bat STING drives IFN-beta production in anti-RNA virus innate immune response by Feiyu Fu, Qi Shao, Jianjian Zhang, Jie Wang, Zhaofei Wang, Jingjiao Ma, Yaxian Yan, Jianhe Sun, Yuqiang Cheng

“…In this study, we identified and cloned the STING gene of the Brazilian free-tailed bat Tadarida brasiliensis (T. brasiliensis) and tested its ability to induce IFN-β by overexpressing and knocking down bat STING (BatSTING) in T. brasiliensis 1 lung (TB1 Lu) cells. …”
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Bacterial meningitis in the early postnatal mouse studied at single-cell resolution by Jie Wang, Amir Rattner, Jeremy Nathans

“…The vascular response to infection appears to be largely driven by TLR4 signaling, as determined by the nearly identical responses induced by infection and LPS administration and by the blunted response to infection in Tlr4-/- mice. Interestingly, knocking out Ccr2, encoding a major chemoattractant for monocytes, or acute depletion of leptomeningeal macrophages, following intracebroventricular injection of liposomal clodronate, had little or no effect on the response of leptomeningeal ECs to E. coli infection. …”
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Human circular RNA hsa_circRNA_101705 (circTXNDC11) regulates renal cancer progression by regulating MAPK/ERK pathway by Chong-Yi Yang, Jie Wang, Jian-Qiu Zhang, Hong-miao Cai

“…The overexpression of circTXNDC11 is linked to advanced TNM stage and lymph node metastasis of renal cancer. Knocking down circTXNDC11 suppressed cell proliferation and invasion in vitro and reduced tumor growth in vivo. …”
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Exosomes Derived from Adipose Tissue-Derived Mesenchymal Stromal Cells Prevent Medication-Related Osteonecrosis of the Jaw through IL-1RA by Yi Zheng, Xian Dong, Xinyu Wang, Jie Wang, Shuo Chen, Yang He, Jingang An, Linhai He, Yi Zhang

“…Interleukin-1 receptor antagonist (IL-1RA)-siRNA was used to knock down the expression of IL-1RA in MSC(AT)s-Exo. …”
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ECM1 regulates the resistance of colorectal cancer to 5-FU treatment by modulating apoptotic cell death and epithelial-mesenchymal transition induction by Sirui Long, Sirui Long, Jie Wang, Jie Wang, Fanbin Weng, Fanbin Weng, Zhigang Pei, Zhigang Pei, Shixian Zhou, Shixian Zhou, Guiyin Sun, Guiyin Sun, Debing Xiang, Debing Xiang

“…From a mechanistic perspective, knocking down and overexpressing ECM1 in HCT15/FU and HCT15 cell lines inhibited and activated PI3K/AKT/GSK3β signaling, respectively. …”
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KRAS mutation-induced EndMT of brain arteriovenous malformation is mediated through the TGF-β/BMP-SMAD4 pathway by Hao Li, Shuo Wang, Jie Wang, Yong Cao, Yuming Jiao, Jiancong Weng, Ran Huo, Shaozhi Zhao, Hongyuan Xu, Zihan Yan, Junze Zhang, Qiheng He, Yingfan Sun

“…Transforming growth factor beta (TGF-β) and bone morphogenetic protein (BMP) signalling pathways were activated in the KRASG12D-mutant HUVECs and ECs in bAVM tissue. Knocking down SMAD4 expression in both KRASG12D-mutant HUVECs and ECs in bAVM tissues inhibited the EndMT. …”
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CDK5 destabilizes PD-L1 via chaperon-mediated autophagy to control cancer immune surveillance in hepatocellular carcinoma by Guoqiang Zhang, Jie Wang, Yu Du, Lei Lv, Yixin Wu, Ruonan Zhang, Ze Yu, Yihan Wang, Yixiao Jiang, Ting Le, Ziqi Li, Haijie Ma

“…Despite the impact on post-translational modifications of PD-L1 being substantial, its significance in resistance to HCC immunotherapy remains poorly defined.Methods Cyclin-dependent kinase 5 (CDK5) expression was knocked down in HCC cells, CDK5 and PD-L1 protein levels were examined by Western blot. …”
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FLI1 induces erythroleukemia through opposing effects on UBASH3A and UBASH3B expression by Jie Wang, Chunlin Wang, Anling Hu, Kunlin Yu, Yi Kuang, Babu Gajendran, Eldad Zacksenhaus, Klarke Michael Sample, Xiao Xiao, Wuling Liu, Yaacov Ben-David

“…RNAseq analysis combined with bioinformatic was used to determine the effect of knocking-down UBASH3A and UBASH3B in leukemic cells. …”
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MSI2 deficiency in ILC3s attenuates DSS-induced colitis by affecting the intestinal microbiota by Nengneng Li, Nengneng Li, Shiquan Xu, Shiquan Xu, Shuaishuai Zhang, Shuaishuai Zhang, Qiang Zhu, Qiang Zhu, Xiaole Meng, Xiaole Meng, Wenbin An, Wenbin An, Baoqing Fu, Baoqing Fu, Baoqing Fu, Mengya Zhong, Yan Yang, Zeyang Lin, Xueni Liu, Junjie Xia, Jie Wang, Jie Wang, Tingting You, Tingting You, Changxiu Yan, Huamei Tang, Guohong Zhuang, Zhihai Peng, Zhihai Peng, Zhihai Peng

“…The species, quantity and function of the intestinal microbiota were characterized by high-throughput 16S rRNA gene sequencing of DNA extracted from fecal samples.ResultsMSI2 was knocked out in the ILC3s of Msi2ΔRorc mice. The Msi2ΔRorc mice exhibited reductions in body weight loss, the disease activity index, degree of colon shortening, tissue histopathological score and immune cells in the peripheral blood compared to those of Msi2fl/fl mice after DSS administration. …”
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