Lipidomic changes of cerebral cortex in aldehyde dehydrogenase-2 knock-in heterozygote mice after chronic alcohol exposure by Li Xiao, Jin Xiang, Xinyu Liu, Lin Yang, Ying Wei, Shiyong Fang, Jing Li, Yi Ye

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Improving the Rice Photosynthetic Efficiency and Yield by Editing <i>OsHXK1</i> via CRISPR/Cas9 System by Shaoyan Zheng, Chanjuan Ye, Jingqin Lu, Jiamin Liufu, Lin Lin, Zequn Dong, Jing Li, Chuxiong Zhuang

“…In this paper, the hexokinase gene <i>OsHXK1</i> was knocked out via the CRISPR/Cas9 gene-editing method in the <i>indica</i> rice varieties Huanghuazhan, Meixiangzhan, and Wushansimiao, and <i>OsHXK1</i>-CRISPR/Cas9 lines were obtained. …”
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The Complement Component 4 Binding Protein α Gene: A Versatile Immune Gene That Influences Lipid Metabolism in Bovine Mammary Epithelial Cell Lines by Xuanxu Chen, Zhihui Zhao, Xinyi Jiang, Jing Li, Fengshuai Miao, Haibin Yu, Ziwei Lin, Ping Jiang

“…The expressions of 3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (HMGCS1), Carnitine Palmitoyltransferase 1A (CPT1A), Fatty Acid Desaturase 1 (FADS1), and Stearoyl-Coenzyme A desaturase 1 (SCD1) significantly changed when the <i>C4BPA</i> gene was knocked out. Collectively, <i>C4BPA</i> gene, which is an immune gene, played an important role in lipid metabolism in BMECs. …”
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Simple promotion of Cas9 and Cas12a expression improves gene targeting via an all-in-one strategy by Yiqiu Cheng, Yiqiu Cheng, Lei Zhang, Lei Zhang, Jing Li, Jing Li, Xiaofei Dang, Jian-Kang Zhu, Jian-Kang Zhu, Hiroaki Shimada, Daisuke Miki

“…We find that facilitating the expression of Cas9 and Cas12a variant by using enhancers can improve DSB and subsequent knock-in efficiency in the Arabidopsis genome. These results indicate that simply increasing Cas protein expression at specific timings - egg cells and early embryos - can improve the establishment of heritable GTs. …”
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Simulated Microgravity Increases the Permeability of HUVEC Monolayer through Up-Regulation of Rap1GAP and Decreased Rap2 Activation by Shuliang Shi, Jing Li, Erzhuo Li, Wenqi Guo, Yao He, Jinpeng Wang, Yao Zhang, Lei Yue, Lijun Wei

“…The results indicate that knock-down of Rap1GAP reduced SMG-induced leaking of the HUVEC monolayer in a time-dependent manner. …”
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High-efficiency targeted transgene integration via primed micro-homologues by Chenxin Wang, Sen Fang, Yangcan Chen, Na Tang, Guanyi Jiao, Yanping Hu, Jing Li, Qingtong Shan, Xin Wang, Guihai Feng, Qi Zhou, Wei Li

“…Here, we exploit prime editors to devise a robust knock-in (KI) strategy named primed micro-homologues-assisted integration (PAINT), which utilizes reverse-transcribed single-stranded micro-homologues to boost targeted KIs in different types of cells. …”
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RNA Sequencing Demonstrates That Circular RNA Regulates Avian Influenza Virus Replication in Human Cells by Jie Min, Ying Cao, Haizhou Liu, Di Liu, Wenjun Liu, Jing Li

“…We further confirmed that expression of the selected circRNAs only increased following AIV infection. Knocking down the selected circRNAs promoted AIV proliferation, and overexpression of three of the candidate circRNAs restricted AIV replication and proliferation. …”
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Application of multiple sgRNAs boosts efficiency of CRISPR/Cas9-mediated gene targeting in Arabidopsis by Jing Li, Dali Kong, Yongping Ke, Wenjie Zeng, Daisuke Miki

“…Abstract Background Precise gene targeting (GT) is a powerful tool for heritable precision genome engineering, enabling knock-in or replacement of the endogenous sequence via homologous recombination. …”
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ZmARF1 positively regulates low phosphorus stress tolerance via modulating lateral root development in maize. by Fengkai Wu, Baba Salifu Yahaya, Ying Gong, Bing He, Junlin Gou, Yafeng He, Jing Li, Yan Kang, Jie Xu, Qingjun Wang, Xuanjun Feng, Qi Tang, Yaxi Liu, Yanli Lu

“…This superior root morphology and greater phosphate stress tolerance could be ascribed to the overexpression of ZmARF1. The knock out mutant zmarf1 had shorter primary roots, fewer root tip number, and lower root volume and surface area. …”
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Vitamin D Improves Cognitive Impairment and Alleviates Ferroptosis via the Nrf2 Signaling Pathway in Aging Mice by Jiaxin Li, Yang Cao, Jie Xu, Jing Li, Chunmei Lv, Qiang Gao, Chi Zhang, Chongfei Jin, Ran Wang, Runsheng Jiao, Hui Zhu

“…Notably, when the VDR was knocked down, VD lost its ability to activate Nrf2. …”
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