The Integration of Gold Nanoparticles with Polymerase Chain Reaction for Constructing Colorimetric Sensing Platforms for Detection of Health-Related DNA and Proteins by Wanhe Wang, Xueliang Wang, Jingqi Liu, Chuankai Lin, Jianhua Liu, Jing Wang

“…Polymerase chain reaction (PCR) is the standard tool in genetic information analysis, and the desirable detection merits of PCR have been extended to disease-related protein analysis. …”
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A comprehensive preimplantation genetic testing approach for SEA-type α-thalassemia by fluorescent gap-polymerase chain reaction combined with haplotype analysis by Jing Wang, Yuanlin Ma, Jing Guo, Rong Li, Canquan Zhou, Yanwen Xu

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Aberrant expression of PROS1 correlates with human papillary thyroid cancer progression by Jing Wang, Minxiang Lei, Zhijie Xu

“…Quantitative real-time polymerase chain reaction (RT-qPCR) and immunohistochemistry (IHC) have been conducted to prove the high expression of PROS1 in PTC. …”
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A prenatal case misunderstood as specimen confusion: 46,XY/46,XY chimerism by Lin Chen, Li Wang, Yang Zeng, Daishu Yin, Feng Tang, Dan Xie, Hongmei Zhu, Lingping Li, Jing Wang

“…Here, we report a case in which the fetus was identified as having tetragametic chimerism based on short tandem repeat - polymerase chain reaction analysis of the family observed during amniocentesis for advanced maternal age. …”
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Paradoxical expressions of natriuretic peptide receptor-C and neutral endopeptidase account for C-type natriuretic peptide decline during the progression of experimental obstructiv... by Peng Hu, Xue Qi Zhao, Jing Wang, Hai Bo Kong, Bo Hu, Ling Lu, Yuan Han Qin

“…Results: The expressions of CNP mRNA and protein in the obstructed kidneys tended to be higher immediately after ligation and declined at later time points compared to sham-operated rats, measured by real-time polymerase chain reaction (PCR) and western blot analysis. …”
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ADAM33 Silencing Inhibits Vascular Smooth Muscle Cell Migration and Regulates Cytokine Secretion in Airway Vascular Remodeling via the PI3K/AKT/mTOR Pathway by Fang Yan, Xin Hu, Long He, Kegang Jiao, Yanyan Hao, Jing Wang

“…Reverse transcription-quantitative polymerase chain reaction and Western blot assays were performed to detect mRNA and protein expression levels. …”
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Circ_0099630 Participates in SPRY1-Mediated Repression in Periodontitis by Jing Wang, Zhenning Wang, Meng Huang, Yu Zhang, Lulu Xu

“…Expression levels of circ_0099630 in isolated PDLCs were assessed using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Effects of circ_0099630 overexpression and silencing on iPDLC viability, proliferation, and cycle progression were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 5-ethynyl-2’-deoxyuridine (EdU), and flow cytometry assays. …”
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Rapid diagnosis of non-tuberculous mycobacterial pulmonary diseases by metagenomic next-generation sequencing in non-referral hospitals by Jing Wang, Huan Xu, Xi Wang, Jian Lan

“…Clinical data, including symptoms, laboratory tests, dynamic computed tomography imaging, treatment, and outcome, were recorded and analyzed.ResultsThe results of mNGS were all positive, with the standard specifically mapped read numbers (SDSMRN) of NTM ranging from 1 to 766; this was confirmed in six patients via quantitative polymerase chain reaction (qPCR) analysis. The duration fromsample collection tomNGS results was 1–4 days. …”
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Circ_0087199 depletion attenuates lipopolysaccharides‐induced human periodontal ligament cell injury through the miR‐527/TLR4 axis by Jing Wang, Na Huo, Chuan Cai, Yu Zhang, Rui Xiao

“…Quantitative real‐time polymerase chain reaction was used to detect the expression of circ_0087199, miR‐527, toll‐like receptor 4 (TLR4). …”
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NQO1 regulates expression and alternative splicing of apoptotic genes associated with Alzheimer's disease in PC12 cells by Yingshi Du, Gejing Liu, Dong Chen, Jinggang Yang, Jing Wang, Yue Sun, Qian Zhang, Yongming Liu

“…Reverse transcription quantitative polymerase chain reaction was performed to explore the impact of NQO1 on the transcription and alternative splicing of apoptotic genes. …”
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microRNA-338-3p suppresses lipopolysaccharide-induced inflammatory response in HK-2 cells by Jing Wang, Guokai Li, Min Lin, Sheng Lin, Ling Wu

“…The production of inflammatory factors and the levels of p38, p65, phospho-p65, phospho-p38, Bax, Bcl-2, cleaved caspase-9, and cleaved caspase-3 were investigated using real-time polymerase chain reaction, western blotting, or enzyme-linked immunosorbent assay. …”
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Chronic Stress Blocks the Endometriosis Immune Response by Metabolic Reprogramming by Chong Lu, Jing Xu, Ke Li, Jing Wang, Yilin Dai, Yiqing Chen, Ranran Chai, Congjian Xu, Yu Kang

“…In addition, the reliability of the mRNA Human Gene Expression Microarray analysis was verified by using research on metabolites based on both the liquid chromatography (LC-MS/MS) technique and quantitative reverse transcription polymerase chain reaction (RT–PCR). A microarray analysis of significantly up-regulated, differentially expressed genes between the chronic stress and the control groups showed genes that were principally related to metabolism-related processes and immune-related processes, such as the immune response process, negative regulation of T cell proliferation, the leucine metabolic process, and the L-cysteine metabolic process (<i>p</i> < 0.05). …”
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A risk score system based on a six-microRNA signature predicts the overall survival of patients with ovarian cancer by Min Zhou, Tao Wu, Yuan Yuan, Shu-Juan Dong, Zhi-Ming Zhang, Yan Wang, Jing Wang

“…A total of 172 ovarian cancer samples and 162 normal ovarian tissues were used to verify the credibility and accuracy of the selected markers of the TCGA cohort by quantitative real-time polymerase chain reaction (PCR). Results We successfully established a risk score system based on a six-microRNA signature (hsa-miR-3074-5p, hsa-miR-758-3p, hsa-miR-877-5p, hsa-miR-760, hsa-miR-342-5p, and hsa-miR-6509-5p). …”
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Circular RNA Expression Profiling and Selection of Key Circular RNAs in the Hypothalamus of Heat-Acclimated Rats by Lijun Fan, Lijun Fan, Gaihong An, Shang Wang, Xuewei Chen, Ying Liu, Zhifeng Liu, Qiang Ma, Jing Wang

“…Differential circRNAs and their regulated downstream miRNAs and mRNAs were quantitatively validated by means of quantitative polymerase chain reaction in real-time (RT-qPCR). Enzyme-linked immunosorbent assay (ELISA) was then applied to predict the expression of proteins. …”
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Identification of the porcine IG-DMR and abnormal imprinting of DLK1-DIO3 in cloned pigs by Junliang Li, Junliang Li, Dawei Yu, Dawei Yu, Jing Wang, Chongyang Li, Qingwei Wang, Jing Wang, Weihua Du, Shanjiang Zhao, Yunwei Pang, Haisheng Hao, Xueming Zhao, Huabin Zhu, Shijie Li, Huiying Zou

“…Subsequently, single nucleotide polymorphism-based sequencing and bisulfite sequencing polymerase chain reaction were conducted to analyze imprinting and methylation in different types of fibroblasts, as well as wild-type and cloned neonatal pigs. …”
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Enhancement Effects and Mechanism Studies of Two Bismuth-Based Materials Assisted by DMSO and Glycerol in GC-Rich PCR by Zhu Yang, Junlei Yang, Lihuan Yue, Bei Shen, Jing Wang, Yuqing Miao, Ruizhuo Ouyang, Yihong Hu

“…Polymerase chain reaction (PCR) has extensive bioanalytical applications in molecular diagnostics and genomic research studies for rapid detection and precise genomic amplification. …”
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circRNA‐MSR regulates the expression of FBXO21 to inhibit chondrocyte autophagy by targeting miR‐761 in osteoarthritis by Zhen Jia, Jia Liu, Jing Wang

“…Clinical OA tissues were collected, and circRNA‐MSR, miR‐761, and FBXO21 expressions were detected via quantitative real‐time polymerase chain reaction (qRT‐PCR). An in vitro OA model was constructed by treating C28/I2 cells with LPS and treating them with overexpression or knockdown vector of circRNA‐MSR, miR‐761, and FBXO21, and autophagy inhibitor 3‐MA. …”
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Identifying altered developmental pathways in human globoid cell leukodystrophy iPSCs-derived NSCs using transcriptome profiling by Yafeng Lv, Yu Qin, Jing Wang, Guoshuai Tian, Wei Wang, Chunyu Cao, Ye Zhang

“…Among them, 25 differentially expressed genes identified by RNA-sequencing analysis were validated using real-time quantitative polymerase chain reaction analysis. Dozens of pathways involved in neuroactive ligand–receptor interactions, synaptic vesicle cycle signaling, serotonergic synapse signaling, phosphatidylinositol–protein kinase B signaling, and cyclic AMP signaling were identified as potential contributors to GLD pathogenesis. …”
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Epigenetic regulatory mechanism of ADAMTS12 expression in osteoarthritis by Shu Yang, Xuanping Zhou, Zhen Jia, Mali Zhang, Minghao Yuan, Yizhao Zhou, Jing Wang, Duo Xia

“…Signal transducer and activator of transcription 1 (STAT1), ADAMTS12, and methyltransferase-like 3 (METTL3) levels were detected by immunohistochemistry, quantitative polymerase chain reaction (qPCR), western blot, or immunofluorescence assay. …”
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Protein tyrosine phosphatase PTPL1 suppresses lung cancer through Src/ERK/YAP1 signaling by Jing Wang, Shuanghui Li, Xiujuan Zhang, Ning Zhu, Ruzetuoheti Yiminniyaze, Liang Dong, Chengwei Li, Wumaier Gulinuer, Jingwen Xia, Jing Li, Daibing Zhou, Xinning Liu, Youzhi Zhang, Yuanyuan Zhang, Shengqing Li

“…Quantitative real‐time polymerase chain reaction (qRT‐PCR) and western blotting were used to verify the transfection efficacy. …”
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