Development and evaluation of an efficient heterologous gene knock-in reporter system in Lactococcus lactis by Yifei Lu, Hongxiang Yan, Jiezhong Deng, Zhigang Huang, Xurui Jin, Yanlan Yu, Qiwen Hu, Fuquan Hu, Jing Wang

Subjects: Get full text

Wfs1 E864K knock-in mice illuminate the fundamental role of Wfs1 in endocochlear potential production by Elodie M. Richard, Emilie Brun, Julia Korchagina, Lucie Crouzier, Corentin Affortit, Stacy Alves, Chantal Cazevieille, Anne-Laure Mausset-Bonnefont, Marc Lenoir, Jean-Luc Puel, Tangui Maurice, Marc Thiry, Jing Wang, Benjamin Delprat

“…None of the animal models of the pathology are presenting with an early onset HL, impeding the understanding of the role of Wolframin (WFS1), the protein responsible for WS, in the auditory pathway. We generated a knock-in mouse, the Wfs1 E864K line, presenting a human mutation leading to severe deafness in affected individuals. …”
Get full text

3-Phosphoinositide-dependent kinase 1 drives acquired resistance to osimertinib by Ismail M. Meraz, Mourad Majidi, Bingliang Fang, Feng Meng, Lihui Gao, RuPing Shao, Renduo Song, Feng Li, Yonathan Lissanu, Huiqin Chen, Min Jin Ha, Qi Wang, Jing Wang, Elizabeth Shpall, Sung Yun Jung, Franziska Haderk, Philippe Gui, Jonathan Wesley Riess, Victor Olivas, Trever G. Bivona, Jack A. Roth

“…PDK1 knock-out dysregulates PI3K/Akt/mTOR signaling, promotes cell cycle arrest at the G1 phase. …”
Get full text

Inducible <i>Rbpms</i>-CreER^T2 Mouse Line for Studying Gene Function in Retinal Ganglion Cell Physiology and Disease by Luming Guo, Xiaoling Xie, Jing Wang, Haiyan Xiao, Shuchun Li, Mei Xu, Ebenezer Quainoo, Rithwik Koppaka, Jiaping Zhuo, Sylvia B. Smith, Lin Gan

“…Additionally, both heterozygous and homozygous <i>Rbpms</i>-CreER^T2 knock-in mice showed no detectable defect in the retinal structure, visual function, and transcriptome. …”
Get full text

Interfering with the expression of EEF1D gene enhances the sensitivity of ovarian cancer cells to cisplatin by Qia Xu, Yun Liu, Shenyi Wang, Jing Wang, Liwei Liu, Yin Xu, Yide Qin

“…Methods The EEF1D gene was knocked-down or -out by siRNA or CRISPR/Cas9 respectively in human ovarian cancer cell SKOV3, DDP-resistant subline SKOV3/DDP, and EEF1D gene in human primary ovarian cancer cell from 5 ovarian cancer patients with progressive disease/stable disease (PD/SD) was transiently knocked down by siRNA interference. …”
Get full text

Overexpressed PKM2 promotes macrophage phagocytosis and atherosclerosis by Xiaochen Gai, Fangming Liu, Yuting Wu, Baohui Zhang, Bufu Tang, Kezhuo Shang, Lianmei Wang, Haihong Zhang, Yixin Chen, Shuhui Yang, Weiwei Deng, Peng Li, Jing Wang, Hongbing Zhang

“…Wild‐type and PKM2 knock‐in macrophages were isolated to assess the function of PKM2 in macrophage phagocytosis. …”
Get full text

Transcription factor Foxp1 stimulates angiogenesis in adult rats after myocardial infarction by Dinghui Wang, Bin Liu, Tianhua Xiong, Wenlong Yu, Huiping Yang, Jing Wang, Xiaodong Jing, Qiang She

“…Adult male rats were subjected to MI, and Foxp1 was knocked down with lentivirus FoxP1 siRNA. Endothelial cell proliferation, angiogenesis, and cardiac function were also assessed. …”
Get full text

Chorionic villus-derived mesenchymal stem cell-mediated NRG1 upregulation promotes HTR-8/SVneo cells proliferation through the activation of the NF-κB signaling pathway by Yajun Liu, Ning Zhang, Yuting Wang, Jianxin Zuo, Jing Wang, Yijing Chu, Yuanhua Ye

“…Of the differentially expressed genes identified when comparing these two groups of cells, 23 proliferation-associated genes were identified and knocked down to test their functional roles in trophoblasts. …”
Get full text

Overexpression of the lncRNA HOTAIRM1 promotes lenvatinib resistance by downregulating miR-34a and activating autophagy in hepatocellular carcinoma by Danyan Gu, Meng Tong, Jing Wang, Bocheng Zhang, Jinghua Liu, Guoqiang Song, Biao Zhu

“…Further study indicated that knocking down HOTAIRM1 in lenvatinib-resistant cell lines increased the level of miR-34a and inhibited the expression of Beclin-1 in Huh7-R and HepG2-R cells. …”
Get full text

Down-Regulation of Double C2 Domain Alpha Promotes the Formation of Hyperplastic Nerve Fibers in Aganglionic Segments of Hirschsprung’s Disease by Jun Xiao, Xinyao Meng, Ke Chen, Jing Wang, Luyao Wu, Yingjian Chen, Xiaosi Yu, Jiexiong Feng, Zhi Li

“…The expression of UNC13B was up-regulated with the knocking down of DOC2A, and there were protein interactions between DOC2A and UNC13B. …”
Get full text

Consanguineous familial study revealed biallelic FIGLA mutation associated with premature ovarian insufficiency by Beili Chen, Lin Li, Jing Wang, Tengyan Li, Hong Pan, Beihong Liu, Yiran Zhou, Yunxia Cao, Binbin Wang

“…The mutation in the start codon of the FIGLA gene alters the open reading frame, leading to a FIGLA knock-out like phenotype. Conclusions Biallelic mutations in FIGLA may be the cause of POI. …”
Get full text

Ferroptotic alveolar epithelial type II cells drive TH2 and TH17 mixed asthma triggered by birch pollen allergen Bet v 1 by Linyi Ma, Ying He, Huancheng Xie, Jing Wang, Jiaqian Chen, Shijie Song, Le Zhang, Linmei Li, He Lai, Yongping Liu, Huifang Chen, Xueyan Zhang, Xueting Liu, Zehong Zou, Qingling Zhang, Jie Yan, Ailin Tao

“…Further analysis revealed that treatment with ferrostatin-1 reduced TH2/TH17-related inflammation and alleviated epithelial damage in mice with Bet v 1-induced asthma. In addition, ACSL4-knocked-down A549 cells are more resistant to Bet v 1-induced ferroptosis. …”
Get full text

Therapeutic TNF Inhibitors Exhibit Differential Levels of Efficacy in Accelerating Cutaneous Wound Healing by Yonghao Cao, Bohdan P. Harvey, Liang Jin, Susan Westmoreland, Jing Wang, Munish Puri, Yingli Yang, Holly M. Robb, Sultan Tanriverdi, Chenqi Hu, Xue Wang, Xiaofeng Xin, Yingchun Liu, Michael P. Macoritto, Kathleen M. Smith, Yu Tian, Kevin White, Timothy R.D.J. Radstake, Zehra Kaymakcalan

“…To examine and compare the efficacy of various TNF inhibitors in cutaneous wound healing in vivo, a human TNF knock-in Leprdb/db mouse model was established to model the impaired cutaneous wound healing as seen in hidradenitis suppurativa. …”
Get full text

ZC3H4 regulates infiltrating monocytes, attenuating pulmonary fibrosis through IL-10 by Yaping Liu, Xinxin Zhang, Jing Wang, Fuhuang Yang, Wei Luo, Jie Huang, Mengling Chen, Sha Wang, Caolong Li, Wei Zhang, Jie Chao

“…The CRISPR/Cas9 system was used to specifically knock down ZC3H4, a novel member of the CCCH zinc finger protein family, and was combined with pharmacological methods to explore the mechanism by which ZC3H4 affects chemokine and cytokine secretion. …”
Get full text

Epigallocatechin Gallate Attenuates Gentamicin-Induced Nephrotoxicity by Suppressing Apoptosis and Ferroptosis by Lin Yue, Ya-Ru Yang, Wen-Xian Ma, Hong-Yan Wang, Qian-Wen Fan, Yue-Yue Wang, Chao Li, Jing Wang, Zi-Mu Hu, Xue-Fu Wang, Feng-He Li, Ming-Ming Liu, Juan Jin, Chao Shi, Jia-Gen Wen

“…However, when Nrf2 was knocked down by siRNA, the above protective effects of EG were weakened. …”
Get full text

Evodiamine inhibits EPRS expression to regulate glutamate metabolism and proliferation of oral squamous cell carcinoma cells by Li‐Qi Lin, Si‐Yi Lv, Hao‐Zhe Ren, Rong‐Rong Li, Lin Li, Yun‐Qing Pang, Jing Wang

“…Glutamate measurements were performed to evaluate the impact of evodiamine on glutamate metabolism of Cal27 and SAS tumor cells. transient transfection techniques were used to knock down and modulate EPRS in these cells. EPRS is expressed at higher levels in OSCC than in normal tissues, and it predicts poor prognosis in patients. …”
Get full text

Cathepsin B-activatable cyclic antisense oligonucleotides for cell-specific target gene knockdown in vitro and in vivo by Zhongyu Wang, Xinli Fan, Guanqun Mu, Xiaoran Zhao, Qian Wang, Jing Wang, Xinjing Tang

“…CB-activatable cASOs were used to successfully knock down two disease-related endogenous genes in CB-abundant PC-3 tumor cells at the mRNA and protein levels but had much less effect on gene knockdown in CB-deficient human umbilical vein endothelial cell (HUVECs). …”
Get full text

Epigenetic regulatory mechanism of ADAMTS12 expression in osteoarthritis by Shu Yang, Xuanping Zhou, Zhen Jia, Mali Zhang, Minghao Yuan, Yizhao Zhou, Jing Wang, Duo Xia

“…ADAMTS12 expression was reduced and the IL-1β-induced inflammatory chondrocyte injury was attenuated by silencing METTL3. Additionally, knocking down METTL3 in ACL-T-produced OA rats reduced ADAMTS12 expression in their cartilage tissues, thereby alleviating cartilage damage. …”
Get full text

Curcumin reverses erastin-induced chondrocyte ferroptosis by upregulating Nrf2 by Yizhao Zhou, Zhen Jia, Jing Wang, Shu Huang, Shu Yang, Sheng Xiao, Duo Xia, Yi Zhou

“…The ferroptosis-related proteins, such as SLC7A11, GPX4, TFR1, ACSL4, and FTH1, were examined using immunofluorescence and western blotting. Nrf2 was knocked down using siRNA to explore the molecular mechanism through which curcumin protects chondrocytes from erastin-induced ferroptosis. …”
Get full text