CTSL, a prognostic marker of breast cancer, that promotes proliferation, migration, and invasion in cells in triple-negative breast cancer by Lianmei Zhang, Lianmei Zhang, Lianmei Zhang, Yang Zhao, Jing Yang, Jing Yang, Yaning Zhu, Ting Li, Xiaoyan Liu, Pengfei Zhang, Jingliang Cheng, Suan Sun, Chunli Wei, Junjiang Fu

“…Cell proliferation, migration, and invasion assay were also performed when over-expressed or knocked-down CTSL.ResultsWe found that the level of CTSL in TNBC is significantly higher than that in the matched adjacent tissues, and associated with differentiated degree, TNM Stage, tumor size, and lymph node metastatic status in TNBC patients. …”
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Identification of disulfidptosis-related subtypes, characterization of tumor microenvironment infiltration, and development of a prognosis model in breast cancer by Jiahui Liang, Xin Wang, Jing Yang, Peng Sun, Jingjing Sun, Shengrong Cheng, Jincheng Liu, Zhiyao Ren, Min Ren

“…CCK-8 and a colony assay obtained by knocking down genes and gene sequencing were used to validate the model.ResultTwo DG clusters were identified based on the expression of 11DGs. …”
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Structural variants involved in high-altitude adaptation detected using single-molecule long-read sequencing by Jinlong Shi, Zhilong Jia, Jinxiu Sun, Xiaoreng Wang, Xiaojing Zhao, Chenghui Zhao, Fan Liang, Xinyu Song, Jiawei Guan, Xue Jia, Jing Yang, Qi Chen, Kang Yu, Qian Jia, Jing Wu, Depeng Wang, Yuhui Xiao, Xiaoman Xu, Yinzhe Liu, Shijing Wu, Qin Zhong, Jue Wu, Saijia Cui, Xiaochen Bo, Zhenzhou Wu, Minsung Park, Manolis Kellis, Kunlun He

“…We find a Tibetan-specific deletion disrupts a super-enhancer and downregulates EPAS1 using enhancer reporter, cellular knock-out and DNA pull-down assays. Our study expands the global SV landscape, reveals the role of gene-regulatory circuitry rewiring in human adaptation, and illustrates the diverse functional roles of SVs in human biology.…”
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High Production of Nattokinase via Fed-Batch Fermentation of the γ-PGA-Deficient Strain of <i>Bacillus licheniformis</i> by Xin Li, Jing Yang, Jun Liu, Xiaohui Zhang, Wei Wu, Dazhong Yan, Lihong Miao, Dongbo Cai, Xin Ma, Shouwen Chen

“…The impact of γ-PGA synthesis on NK production remains unclear. In this study, we knocked out the <i>pgsC</i> gene, a component of the γ-PGA synthetase cluster (<i>pgsBCA</i>), and constructed a novel recombinant strain, <i>Bacillus licheniformis</i> BL11. …”
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CXCL2-mediated ATR/CHK1 signaling pathway and platinum resistance in epithelial ovarian cancer by Sipei Nie, Yicong Wan, Hui Wang, Jinhui Liu, Jing Yang, Rui Sun, Huangyang Meng, Xiaolin Ma, Yi Jiang, Wenjun Cheng

“…The functional assays showed overexpressing CXCL2 or co-culturing with recombinant human CXCL2 promoted cell resistance to cisplatin. Conversely, knocking down CXCL2 or co-culturing with neutralizing antibody to CXCL2 increased cell response to cisplatin. …”
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Discovery of a novel megakaryopoiesis enhancer, ingenol, promoting thrombopoiesis through PI3K-Akt signaling independent of thrombopoietin by Long Wang, Ting Zhang, Sha Liu, Qi Mo, Nan Jiang, Qi Chen, Jing Yang, Yun-Wei Han, Jian-Ping Chen, Fei-Hong Huang, Hua Li, Jie Zhou, Jie-Si Luo, Jian-Ming Wu

“…Furthermore, a RIT mice model and c-MPL knock-out (c-MPL-/-) mice constructed by CRISPR/Cas9 technology were used to assess the therapeutic action of ingenol on thrombocytopenia. …”
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Identification and Validation of a Prognostic Signature for Thyroid Cancer Based on Ferroptosis-Related Genes by Yue Wang, Jing Yang, Shitu Chen, Weibin Wang, Lisong Teng

“…By combining gene expression and clinical parameters, we developed a ferroptosis-related gene-based prognostic model with an area under the curve (AUC) of 0.816, and the nomogram also achieved good predictive efficacy for the three-year survival rate of thyroid cancer patients. Knocking down AKR1C3 enhances thyroid cancer cell proliferation, invasion, and migration abilities. …”
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Dysfunction of Akt/FoxO3a/Atg7 regulatory loop magnifies obesity-regulated muscular mass decline by Yang Yu, Jing Yang, Lixia Zheng, Han Su, Sunrun Cao, Xuehan Jiang, Xiyan Liu, Weiwei Liu, Zhuo Wang, Fang Meng, Hongde Xu, Deliang Wen, Chen Sun, Xiaoyu Song, Antonio Vidal-Puig, Liu Cao

“…Methods: In this study, we established a murine model of high-fat diet-induced obesity (DIO) and introduced adeno-associated virus delivery of short hairpin RNA to knock down Atg7 (shAtg7) into the gastrocnemius muscle. …”
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Gut microbiota-related metabolite alpha-linolenic acid mitigates intestinal inflammation induced by oral infection with Toxoplasma gondii by Jing Yang, Songhao Liu, Qian Zhao, Xiaobing Li, Kangfeng Jiang

“…In our previous study, there was a decrease in the severity of toxoplasmosis after T. gondii α-amylase (α-AMY) was knocked out. Here, we established mouse models of ME49 and Δα-amy cyst infection and then took advantage of 16S rRNA gene sequencing and metabolomics analysis to identify specific gut microbiota-related metabolites that mitigate T. gondii-induced intestinal inflammation and analyzed the underlying mechanism. …”
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<i>Toxoplasma gondii</i> <i>ADSL</i> Knockout Provides Excellent Immune Protection against a Variety of Strains by Luyao Wang, Ding Tang, Chenghang Yang, Jing Yang, Rui Fang

“…In addition, ideal vaccine production is a challenge in the future. In this study, we knocked out the adenylosuccinate lyase (<i>ADSL</i>) gene and found that the gene reduces the growth rate of <i>T. gondii</i> tachyzoites in vitro under standard growth conditions by plaque or replication experiments. …”
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Interferon-tau protects bovine endometrial epithelial cells against inflammatory injury by regulating the PI3K/AKT/β-catenin/FoxO1 signaling axis by Kangfeng Jiang, Jinyin Cai, Qianming Jiang, Juan J. Loor, Ganzhen Deng, Xiaobing Li, Jing Yang

“…To explore the role of FoxO1 on the inhibition of TLR4 by IFN-τ, we employed LY294002 to inhibit the PI3K/AKT while FoxO1 was knocked down. Results revealed that the knockdown of FoxO1 blocked the upregulation of TLR4 and p-p65 induced by LY294002, and enhanced the inhibition of IFN-τ on TLR4, p-p65, and cell apoptosis. …”
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