Localisation of collagenase within islets following infusion into human pancreas by Cross, S, Hughes, S, Partridge, C, Clark, A, Gray, D, Johnson, P

Influence of neutral protease concentration on the digestion of collagen VI within the human pancreas by Gray, J, Hughes, S, Cross, SE, Gray, D, Clark, A, Johnson, P

Collagen VI within the islet-exocrine interface of the human pancreas is not resistant to liberase digestion by Hughes, S, Cross, S, Gray, D, Clark, A, Johnson, P

Donor pancreata: evolving approaches to organ allocation for whole pancreas versus islet transplantation. by Berney, T, Johnson, P

“…The preferential allocation of pancreases from obese and older donors for islet isolation has been based on their association with worse pancreas transplant outcomes and better islet isolation results in islet yields. …”

Development of an assay for the detailed study of collagenase-digestion within the islet-exocrine interface of the human pancreas by Hughes, S, Cross, S, Gray, D, Clark, A, Johnson, P

Quality of Isolated Pig Islets is Improved Using Perfluorohexyloctane for Pancreas Storage in a Split Lobe Model by Brandhorst, H, Iken, M, Scott, W, Papas, K, Theisinger, B, Johnson, P, Korsgren, O, Brandhorst, D

“…Pancreas transportation between donor center and islet production facility is frequently associated with prolonged ischemia impairing islet isolation and transplantation outcomes. …”

Quality of isolated pig islets is improved using perfluorohexyloctane for pancreas storage in a split lobe model. by Brandhorst, H, Iken, M, Scott, W, Papas, K, Theisinger, B, Johnson, P, Korsgren, O, Brandhorst, D

“…Pancreas transportation between donor center and islet production facility is frequently associated with prolonged ischemia impairing islet isolation and transplantation outcomes. …”

Reprogramming spleen into pancreas: a novel study of the molecular mechanisms behind splenic mesenchyme-to-epithelial transition by Robertson, SA, Rowan-Hull, A, D'Alessandro, P, Ramnath, R, Johnson, P

Characterisation of collagen VI within the islet-exocrine interface of the human pancreas: implications for clinical islet isolation? by Hughes, S, Clark, A, McShane, P, Contractor, H, Gray, D, Johnson, P

“…In this study, the composition of collagen subtypes in the interface have been characterized and quantified in human pancreas. METHODS: Human adult pancreases were retrieved from older (mean age 55.7+/-3.0 yrs) and young donors (mean age 21.8+/-3.2 yrs). …”

Comparison of the collagen VI content within the islet-exocrine interface of the head, body, and tail regions of the human pancreas. by Hughes, S, McShane, P, Contractor, H, Gray, D, Clark, A, Johnson, P

“…Recently it has been reported that transplantable yields of islets can be isolated from the tail segment of the pancreas alone. This study aimed to quantify and compare the amount of collagenase-resistant collagen VI within the islet-exocrine interface of the head, body, and tail of the human pancreas. …”

Quantifying the effects of different neutral proteases on human islet integrity by Brandhorst, H, Johnson, P, Korsgren, O, Brandhorst, D

“…Efficient islet release from the pancreas requires the combination of collagenase, neutral protease (cNP), or thermolysin (TL). …”

Factors influencing the collagenase digestion phase of human islet isolation. by Kin, T, Johnson, P, Shapiro, A, Lakey, JR

“…However, it is still difficult to recover the entire quantity of islets contained in a pancreas. A major obstacle to successful human islet isolation has been the variability of the collagenase digestion phase of islet isolation. …”

Foregut mesenchyme contributes cells to pancreatic acini during embryonic development in a chick-quail chimera model. by Teague, W, Jayanthi, N, Lear, P, Johnson, P

“…To understand causes of developmental abnormalities of the pancreas, it is essential to understand its normal embryonic development. …”

Development of a novel digestion chamber for human and porcine islet isolation. by Gray, D, Sudhakaran, N, Titus, T, McShane, P, Johnson, P

“…BACKGROUND: The current technique of human pancreas digestion for islet isolation relies on selective distribution of collagenase delivered via the pancreatic duct to produce digestion and removal of peri-acinar fibrous tissue. …”

Altered phenotype of β-cells and other pancreatic cell lineages in patients with diffuse congenital hyperinsulinism in infancy caused by mutations in the ATP-sensitive K-channel by Salisbury, R, Han, B, Jennings, R, Berry, A, Stevens, A, Mohamed, Z, Sugden, S, De Krijger, R, Cross, S, Johnson, P, Newbould, M, Cosgrove, K, Hanley, K, Banerjee, I, Dunne, M, Hanley, N

“…A tendency toward increased NKX2.2 expression was consistent with its detection in two-thirds of CHI-D δ-cell nuclei, similar to the fetal pancreas, and implied immature δ-cell function. CHI-D δ-cells also comprised 10% of cells displaying nucleomegaly. …”

Insights into the molecular mechanism for type 2 diabetes susceptibility at the KCNQ1 locus from temporal changes in imprinting status in human islets. by Travers, M, Mackay, D, Dekker Nitert, M, Morris, A, Lindgren, C, Berry, A, Johnson, P, Hanley, N, Groop, L, McCarthy, M, Gloyn, A

“…Genotype at the type 2 diabetes risk variant rs2237895 influenced methylation levels of regulatory sequence in fetal pancreas but without demonstrable effects on gene expression. …”

Pancreatic L-Glutamine Administration Protects Pig Islets from Cold Ischemic Injury and Increases Resistance toward Inflammatory Mediators by Brandhorst, H, Theisinger, B, Guenther, B, Johnson, P, Brandhorst, D

“…In the present study we investigated whether pancreas oxygenation can be efficiently combined with an antioxidant strategy utilizing intraductal L-glutamine administration. …”

Multicenter assessment of animal-free collagenase AF-1 for human islet isolation by Brandhorst, D, Parnaud, G, Friberg, A, Lavallard, V, Demuylder-Mischler, S, Hughes, S, Saphörster, J, Kurfürst, M, Korsgren, O, Berney, T, Johnson, P

“…This report describes the initial experiences of 3 different islet transplant centers. Thirty-four human pancreases were digested using 1 vial of the 6 different lots of Collagenase AF-1 (2,000-2,583 PZ-U/vial) supplemented with 4 different lots of NP AF in a range of 50 to 160 DMC-U per pancreas. …”

Pancreatic islet transplantation. by Johnson, P, Jones, K

“…In both scenarios, the treatment involves extracting sufficient numbers of pancreatic islets from the resected or donor pancreas and infusing these through the portal vein into the patient's liver. …”

Islet/stem cell transplantation - what's new? by Spiliotis, I, Johnson, P

“…These include optimising pancreas procurement and islet isolation, improving post-transplant islet engraftment and survival, and the use of renewable sources of islets such as stem cells. …”