An Algorithm to Predict the Lack of Pregnancy after Intrauterine Insemination in Infertile Patients by Emma Garcia-Grau, Mario Oliveira, Maria José Amengual, Encarna Rodriguez-Sanchez, Ana Veraguas-Imbernon, Laura Costa, Jordi Benet, Jordi Ribas-Maynou

Subjects: Get full text

Determination of double- and single-stranded DNA breaks in bovine sperm is predictive of their fertilizing capacity by Jordi Ribas-Maynou, Ariadna Delgado-Bermúdez, Yentel Mateo-Otero, Estel Viñolas, Carlos O. Hidalgo, W. Steven Ward, Marc Yeste

Subjects: Get full text

Relationship of Seminal Oxidation-Reduction Potential with Sperm DNA Integrity and pH in Idiopathic Infertile Patients by Sergio Garcia-Segura, Jordi Ribas-Maynou, Sandra Lara-Cerrillo, Agustín Garcia-Peiró, Ana Belén Castel, Jordi Benet, Maria Oliver-Bonet

Subjects: Get full text

Animal board invited review: An update on the methods for semen quality evaluation in swine – from farm to the lab by Carolina Maside, Sandra Recuero, Albert Salas-Huetos, Jordi Ribas-Maynou, Marc Yeste

“…Considering the high degree of chromatin packaging in pig sperm, which only have protamine 1, growing evidence suggests that complete decondensation of that chromatin is needed before DNA fragmentation through TUNEL or Comet can be examined.…”
Get full text

Sperm DNA damage compromises embryo development, but not oocyte fertilisation in pigs by Yentel Mateo-Otero, Marc Llavanera, Sandra Recuero, Ariadna Delgado-Bermúdez, Isabel Barranco, Jordi Ribas-Maynou, Marc Yeste

“…Finally, the present work also found that the inclusion of Comet assays to the conventional sperm quality tests improves the prediction of blastocyst formation (AUC = 0.9021, P < 0.05), but not fertilisation rates (P > 0.05). …”
Get full text

Proteomic Analysis in Seminal Plasma of Fertile Donors and Infertile Patients with Sperm DNA Fragmentation by Alba Fernandez-Encinas, Agustín García-Peiró, Javier del Rey, Jordi Ribas-Maynou, Carlos Abad, Maria José Amengual, Elena Prada, Joaquima Navarro, Jordi Benet

“…For that, semen samples were selected according to semen analysis, clinical pathology, and values of sperm DNA fragmentation (alkaline and neutral Comet assay and Sperm Chromatin Dispersion test). …”
Get full text

An intracellular, non-oxidative factor activates in vitro chromatin fragmentation in pig sperm by Estel Viñolas-Vergés, Marc Yeste, Ferran Garriga, Sergi Bonet, Yentel Mateo-Otero, Jordi Ribas-Maynou

“…For this purpose, sperm DNA integrity was evaluated through the Comet assay and Pulsed Field Gel Electrophoresis (PFGE); sperm motility and agglutination were assessed with computer assisted sperm analysis (CASA); and sperm viability and levels of total reactive oxygen species (ROS) and superoxides were determined through flow cytometry. …”
Get full text

Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction by Estel Viñolas-Vergés, Jordi Ribas-Maynou, Isabel Barranco, Camila Peres Rubio, Sergi Bonet, Jordi Roca, Marc Yeste

“…Results This study determined chromatin protamination (Chromomycin A3 test, CMA3), condensation (Dibromobimane test, DBB), and DNA integrity (Comet assay) in the pig sperm contained in the first 10 mL of the SRF (SRF-P1), the remaining portion of the sperm-rich fraction (SRF-P2), and the post sperm-rich fraction (PSRF). …”
Get full text

Sperm DNA integrity does play a crucial role for embryo development after ICSI, notably when good-quality oocytes from young donors are used by Jordi Ribas-Maynou, Sergi Novo, Marc Torres, Albert Salas-Huetos, Sergi Rovira, Marta Antich, Marc Yeste

“…Single- and double-stranded sperm DNA breaks were determined with alkaline and neutral Comet assays, respectively; ICSI was conducted following standard protocols and embryos were monitored through time-lapse microscopy. …”
Get full text