Transcriptional profiling of human liver identifies sex-biased genes associated with polygenic dyslipidemia and coronary artery disease. by Yijing Zhang, Kathrin Klein, Aarathi Sugathan, Najlla Nassery, Alan Dombkowski, Ulrich M Zanger, David J Waxman

“…Sex-differences in human liver gene expression were characterized on a genome-wide scale using a large liver sample collection, allowing for detection of small expression differences with high statistical power. 1,249 sex-biased genes were identified, 70% showing higher expression in females. …”
Get full text

Bioengineered Pancreas–Liver Crosstalk in a Microfluidic Coculture Chip Identifies Human Metabolic Response Signatures in Prediabetic Hyperglycemia by Reza Zandi Shafagh, Sonia Youhanna, Jibbe Keulen, Joanne X. Shen, Nayere Taebnia, Lena C. Preiss, Kathrin Klein, Florian A. Büttner, Mikael Bergqvist, Wouter van derWijngaart, Volker M. Lauschke

“…On‐chip coculture experiments of organotypic primary human liver spheroids and intact primary human islets demonstrate insulin secretion and hepatic insulin response dynamics at physiological timescales upon glucose challenge. …”
Get full text

Saturated Fat-Mediated Upregulation of IL-32 and CCL20 in Hepatocytes Contributes to Higher Expression of These Fibrosis-Driving Molecules in MASLD by Katharina Schilcher, Rania Dayoub, Marion Kubitza, Jakob Riepl, Kathrin Klein, Christa Buechler, Michael Melter, Thomas S. Weiss

“…RT-PCR, ELISA and Western blotting analyses were performed in both human liver samples and an in vitro steatosis model. …”
Get full text

The effect of genetic variants in the transcription factor TSPYL family on the CYP3A4 mediated cyclosporine metabolism in kidney transplant patients by Qinglian Zhai, Dirk Jan A. R. Moes, Teun vanGelder, Maaike van derLee, Jan‐Stephan Sanders, Frederike J. Bemelman, Johan W. deFijter, Kathrin Klein, Matthias Schwab, Jesse J. Swen

“…Here, we investigated the effect of genetic variants in TSPYL on CYP3A4 activity using data from a clinical study and a human liver bank. Five SNVs (rs3828743, rs10223646, rs6909133, rs1204807, and rs1204811) in TSPYL were selected because of a reported effect on CYP3A4 expression in vitro or suggested clinical effect. …”
Get full text

Hepatic Expression of the Na⁺-Taurocholate Cotransporting Polypeptide Is Independent from Genetic Variation by Roman Tremmel, Anne T. Nies, Barbara A. C. van Eijck, Niklas Handin, Mathias Haag, Stefan Winter, Florian A. Büttner, Charlotte Kölz, Franziska Klein, Pascale Mazzola, Ute Hofmann, Kathrin Klein, Per Hoffmann, Markus M. Nöthen, Fabienne Z. Gaugaz, Per Artursson, Matthias Schwab, Elke Schaeffeler

“…<i>SLC10A1</i>/NTCP mRNA expression/protein abundance was quantified in well-characterized 143 human livers by real-time PCR and LC-MS/MS-based targeted proteomics. …”
Get full text