Evaluation of perfluorohexyloctane/polydimethylsiloxane for pancreas preservation for clinical islet isolation and transplantation by Sthle, M, Foss, A, Gustafsson, B, Lempinen, M, Lundgren, T, Rafael, E, Tufveson, G, Theisinger, B, Brandhorst, D, Korsgren, O, Friberg, A

“…Pancreas preservation using F6H8S5 proved as effective as UW and Custadiol when used within CIT up to 10 h, in terms of both isolation outcome and islet functionality. …”

Using HTK for prolonged pancreas preservation prior to human islet isolation by Caballero-Corbalán, J, Brandhorst, H, Malm, H, Felldin, M, Foss, A, Salmela, K, Tibell, A, Tufveson, G, Korsgren, O, Brandhorst, D

Using HTK for prolonged pancreas preservation prior to human islet isolation. by Caballero-Corbalán, J, Brandhorst, H, Malm, H, Felldin, M, Foss, A, Salmela, K, Tibell, A, Tufveson, G, Korsgren, O, Brandhorst, D

Quality of Isolated Pig Islets is Improved Using Perfluorohexyloctane for Pancreas Storage in a Split Lobe Model by Brandhorst, H, Iken, M, Scott, W, Papas, K, Theisinger, B, Johnson, P, Korsgren, O, Brandhorst, D

“…Pancreas transportation between donor center and islet production facility is frequently associated with prolonged ischemia impairing islet isolation and transplantation outcomes. …”

Quality of isolated pig islets is improved using perfluorohexyloctane for pancreas storage in a split lobe model. by Brandhorst, H, Iken, M, Scott, W, Papas, K, Theisinger, B, Johnson, P, Korsgren, O, Brandhorst, D

“…Pancreas transportation between donor center and islet production facility is frequently associated with prolonged ischemia impairing islet isolation and transplantation outcomes. …”

Pig pancreas oxygenation at 20 degrees C increases islet ATP generation but deteriorates islet function. by Iken, M, Brandhorst, H, Korsgren, O, Brandhorst, D

“…Successful pancreas preservation during storage in oxygenated perfluorodecalin (PFD) is mainly related to oxidative ATP generation during storage. …”

The ratio between collagenase class I and class II influences the efficient islet release from the rat pancreas. by Brandhorst, H, Raemsch-Guenther, N, Raemsch, C, Friedrich, O, Huettler, S, Kurfuerst, M, Korsgren, O, Brandhorst, D

“…Because no information has been available, this study was aimed to investigate the isolation efficiency of different ratios between collagenase class II and I (C-ratio) in the rat pancreas serving as model for the human pancreas without being restricted by the large variability observed in human donors. …”

A new oxygen carrier for improved long-term storage of human pancreata before islet isolation. by Brandhorst, H, Asif, S, Andersson, K, Theisinger, B, Andersson, H, Felldin, M, Foss, A, Salmela, K, Tibell, A, Tufveson, G, Korsgren, O, Brandhorst, D

“… BACKGROUND: Pancreas oxygenation during cold storage has been established in islet isolation and transplantation to prevent ischemic tissue damage using perfluorodecalin (PFD) as hyperoxygen carrier. …”

Quantifying the effects of different neutral proteases on human islet integrity by Brandhorst, H, Johnson, P, Korsgren, O, Brandhorst, D

“…Efficient islet release from the pancreas requires the combination of collagenase, neutral protease (cNP), or thermolysin (TL). …”

The importance of tryptic-like activity in purified enzyme blends for efficient islet isolation. by Brandhorst, H, Friberg, A, Andersson, H, Felldin, M, Foss, A, Salmela, K, Lundgren, T, Tibell, A, Tufveson, G, Korsgren, O, Brandhorst, D

“… BACKGROUND: The isolation of islets from the human pancreas critically depends on an efficient enzyme blend. …”

New class of oxygen carriers improves islet isolation from long-term stored rat pancreata. by Brandhorst, H, Muehling, B, Yamaya, H, Henriksnaes, J, Carlsson, P, Korsgren, O, Brandhorst, D

“… OBJECTIVE: Pancreas shipment is frequently associated with prolonged ischemia deteriorating islet graft function. …”

Multicenter assessment of animal-free collagenase AF-1 for human islet isolation by Brandhorst, D, Parnaud, G, Friberg, A, Lavallard, V, Demuylder-Mischler, S, Hughes, S, Saphörster, J, Kurfürst, M, Korsgren, O, Berney, T, Johnson, P

“…This report describes the initial experiences of 3 different islet transplant centers. Thirty-four human pancreases were digested using 1 vial of the 6 different lots of Collagenase AF-1 (2,000-2,583 PZ-U/vial) supplemented with 4 different lots of NP AF in a range of 50 to 160 DMC-U per pancreas. …”

Large-scale comparison of Liberase HI and collagenase NB1 utilized for human islet isolation. by Brandhorst, H, Friberg, A, Friberg, A, Nilsson, B, Nilsson, B, Andersson, H, Andersson, H, Felldin, M, Felldin, M, Foss, A, Foss, A, Salmela, K, Salmela, K, Tibell, A, Tibell, A, Tufveson, G, Tufveson, G, Korsgren, O, Korsgren, O, Brandhorst, D, Brandhorst, D

“…The results suggest that Liberase is more efficient for pancreas dissociation than collagenase NB1 but seems to be more harmful to exocrine cells and islet tissue.…”

Comparison of Clostripain and Neutral Protease as Supplementary Enzymes for Human Islet Isolation by Brandhorst, H, Johnson, P, Mönch, J, Kurfürst, M, Korsgren, O, Brandhorst, D

“…The present study compared islet isolation outcome after pancreas digestion by means of collagenase used alone or supplemented with either neutral protease (NP), clostripain (CP), or both proteases. …”

Perfluorohexyloctane improves long-term storage of rat pancreata for subsequent islet isolation. by Brandhorst, H, Theisinger, B, Yamaya, H, Henriksnäs, J, Carlsson, P, Korsgren, O, Brandhorst, D

“…Pancreas oxygenation by means of the hyperoxygen carrier perfluorodecalin (PFD) has been established to prevent ischemically induced damage from cold-stored pancreata. …”