Loss of function of VdDrs2, a P4-ATPase, impairs the toxin secretion and microsclerotia formation, and decreases the pathogenicity of Verticillium dahliae by Hui Ren, Xianbi Li, Yujie Li, Mengjun Li, Jiyuan Sun, Fanlong Wang, Jianyan Zeng, Yang Chen, Lei Wang, Xingying Yan, Yanhua Fan, Dan Jin, Yan Pei

“…Four P4-ATPase flippase genes, VdDrs2, VdNeo1, VdP4-4, and VdDnf1 were identified in Verticillium dahliae, one of the most devastating phytopathogenic fungi in the world. Knock out of VdDrs2, VdNeo1, and VdP4-4, or knock down of VdDnf1 significantly decreased the pathogenicity of the mutants in cotton. …”
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A Protein Asteroid with PIN Domain in Silkworm <i>Bombyx mori</i> Is Involved in Anti-BmNPV Infection by Yuchen Xia, Mouzhen Jiang, Xiaoxuan Hu, Qing Wang, Cen Qian, Baojian Zhu, Guoqing Wei, Lei Wang

“…The transcriptional levels of the <i>BmAst</i> gene in 5th instar larvae were significantly induced by BmNPV or dsRNA. After knocking down <i>BmAst</i> gene expression by specific dsRNA, the proliferation of BmNPV in <i>B. mori</i> was increased significantly, whereas the survival rate of larvae was significantly lower when compared with the control. …”
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LncRNA LNC-565686 Promotes Proliferation of Prostate Cancer by Inhibiting Apoptosis through Stabilizing SND1 by Xuke Qin, Jiacheng Zhong, Lei Wang, Zhiyuan Chen, Xiuheng Liu

“…In order to further explore the effects of LNC-565686 and SND1 on prostate cancer cells, we knocked down LNC-565686 and SND1 in prostate cancer cells, and verified using CCK8 and flow cytometry and western blot for the detection of apoptosis-related indicators. …”
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Regulatory role of PI16 in autoimmune arthritis and intestinal inflammation: implications for Treg cell differentiation and function by Yuankai Sun, Shiyu Lin, Hui Wang, Lei Wang, Yulu Qiu, Feifei Zhang, Nannan Hao, Fang Wang, Wenfeng Tan

“…Our findings demonstrate that conditional knock-out of PI16 in Tregs significantly enhances their differentiation and suppressive functions. …”
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Bi‐allelic pathogenic variants in PABPC1L cause oocyte maturation arrest and female infertility by Weijie Wang, Jing Guo, Juanzi Shi, Qun Li, Biaobang Chen, Zhiqi Pan, Ronggui Qu, Jing Fu, Rong Shi, Xia Xue, Jian Mu, Zhihua Zhang, Tianyu Wu, Wenjing Wang, Lin Zhao, Qiaoli Li, Lin He, Xiaoxi Sun, Qing Sang, Ge Lin, Lei Wang

“…In vivo, three strains of Pabpc1l knock‐in (KI) female mice were infertile. RNA‐sequencing analysis showed abnormal activation of the Mos‐MAPK pathway in the zygotes of KI mice. …”
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Effect of Temperature Conditions on Flame Evolutions of Turbulent Jet Ignition by Jiaying Pan, Yu He, Tao Li, Haiqiao Wei, Lei Wang, Gequn Shu

“…Turbulent jet ignition technology can significantly improve lean combustion stability and suppress engine knocking. However, the narrow jet channel between the pre-chamber and the main chamber leads to some difficulties in heat exchange, which significantly affects combustion performance and mechanical component lifetime. …”
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COL12A1 Acts as a Novel Prognosis Biomarker and Activates Cancer-Associated Fibroblasts in Pancreatic Cancer through Bioinformatics and Experimental Validation by Yao Song, Lei Wang, Kaidong Wang, Yuhua Lu, Pengcheng Zhou

“…This was verified with our PCR analysis in cancer cells and CAFs. The knocking down of COL12A1 decreased the proliferation and migration of CAFs and down-regulated the expression of CAF activation markers actin alpha 2 (ACTA2), fibroblast activation protein (FAP), and fibroblast-specific protein 1 (FSP1). …”
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<i>NGT1</i> Is Essential for <i>N</i>-Acetylglucosamine-Mediated Filamentous Growth Inhibition and <i>HXK1</i> Functions as a Positive Regulator of Filamentous Growth in <i>Candida... by Qiuyu Zhang, Li Xu, Sheng Yuan, Qinghua Zhou, Xuxia Wang, Lei Wang, Zhiming Hu, Yunjun Yan

“…In the present study, we found that knocking out the GlcNA-specific transporter gene <i>NGT1</i> was sufficient to enhance <i>C. tropicalis</i> filamentous growth on Lee’s plus GlcNAc medium. …”
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Prolyl Hydroxylase 3 Knockdown Accelerates <i>VHL</i>-Mutant Kidney Cancer Growth In Vivo by Niki M. Zacharias, Lei Wang, Tapati Maity, Li Li, Steven W. Millward, Jose A. Karam, Christopher G. Wood, Neema Navai

“…Using viral transduction, we knocked down the expression of PHD3 in the human ccRCC cell line UMRC3. …”
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Optimizing Hexose Utilization Pathways of <i>Cupriavidus necator</i> for Improving Growth and L-Alanine Production under Heterotrophic and Autotrophic Conditions by Lei Wang, Huiying Luo, Bin Yao, Junhu Yao, Jie Zhang

“…To explore the effect of the <i>pfkA</i> gene on the production of <i>C. necator</i>, an alanine-producing <i>C. necator</i> was constructed by expressing the NADPH-dependent L-alanine dehydrogenase, alanine exporter, and knocking out the <i>phaC1AB1</i> operon. The alanine-producing strain had maximum alanine titer and yield of 784 mg/L and 11.0%, respectively. …”
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PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage responseResearch in context by Wei Yue, Xinyu Li, Xiaolu Zhan, Lei Wang, Jihong Ma, Meiyu Bi, Qilong Wang, Xiaoyang Gu, Bingteng Xie, Tong Liu, Hongyan Guo, Xin Zhu, Chen Song, Jie Qiao, Mo Li

“…Methods: A CRISPR-based knock-in technology was used to establish stable BRCA1 mutant cancer cells. …”
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